Graphiola fimbriata: the first species of Graphiolaceae (Exobasidiales, Basidiomycota) described only based on its yeast stage

Abstract

The systematic position of three yeast strains isolated from a plant cell culture, a piece of termite nest, and as a foliar endophyte of Coffea arabica, respectively, is evaluated using morphological, physiological, and phylogenetical analyses. In culture, all three isolates produced white, pale orange to pink colored colonies of cylindrical cells with monopolar budding and pseudohyphae. Standard phenotypic, biochemical, physiological characterization and phylogenetic analyses of the combined 26S rRNA gene (D1/D2 domains) and ITS region sequences showed the conspecificity of these isolates and suggest their placement within the Exobasidiales (Ustilaginomycotina) as a sister lineage of the sampled and sequenced Graphiola species. Here, we describe this species as Graphiola fimbriata sp. nov. MycoBank MB 825077 (holotype: PC1 T ; ex-type cultures: IBRC-M 30158 T = CBS 13945 T = DSM 104832 T). This is the first anamorphic saprobic species described in the genus Graphiola. The description of the genus Graphiola is therefore emended to allow species known only from a saprobic state.

Two new species in a new genus and a critical revision of Brachybasidiaceae (Exobasidiales, Basidiomycota) in honor of Franz Oberwinkler

“…In addition, sequences of all available species of Brachybasidiaceae and Graphiolaceae were added including sequences from the public catalog of the NITE Biological Resource Center collection (NBRC), Japan. GenBank accession numbers of the sequences used (Begerow et al 2002;Boekhout et al 2003;Cao et al 2018;Crous et al 2003;Kottke et al 2010;Kruse et al 2017;Macedo et al 2016;Maier et al 2006;Matheny et al 2006;Nasr et al 2019;Piepenbring et al 2010Piepenbring et al , 2012aSchoch et al 2014;Sepúlveda et al 2017;Tanaka et al 2008;Wang et al 2015;Yasuda et al 2005) are cited in Fig. 2.…”

Species diversity of Basidiomycota

“…Exobasidium species on Ericaceae or Microstroma species on various tree species, for instance, do not produce teliospores anymore, but produce basidia directly from their hyphae through the stomata of their hosts (e.g., Begerow et al 2001Begerow et al , 2002. Additionally, phylogenetic studies have shown that many lineages that are exclusively known as yeasts are found in these groups and it is assumed that several lineages have lost the ability to parasitize plants (Wang et al 2015c;Kijpornyongpan et al 2018;Nasr et al 2019).…”

Natural Products from Endophytic Fungi Associated with Rubiaceae Species

“…-dihydroxybut-3-enyl)-5H-furan-2-one (21) and (2E,4R)-2,4dimethylnon-2-enoic acid (22); seven phenolic derivatives: p-hydroxyphenyllactic acid (23), phenyllactic acid (24), p-hydroxybenzoic acid (25), p-hydroxybenzaldehyde (26), n-butyl-3,4dihydroxybenzoate (27), n-hexyl-3,4-dihydroxybenzoate (28) and n-octyl-3,4-dihydroxybenzoate (29); and the known compound (2E,4S)-2,4-dimethyloct-2-enoic acid (30) ( Figure 5) [102]. Extracts of solid cultures of Sporormiella minimoides (Sporormiaceae), isolated as an endophytic fungus from leaves Hintonia latiflora collected in Mexico, yielded five polyketides, 3,6-dimethoxy-8methyl-1H,6Hbenzo[de]isochromene-1,9-dione (31), 3-hydroxy-1,6,10-trimethoxy-8-methyl-1H,3Hbenzo[de]isochromen-9-one (32), 5-hydroxy-2,7-dimethoxy-8-methylnaphthoquinone (33), minimoidiones A (34) and B (35), along with four known compounds: corymbiferone (36), ziganein (37), brocaenol B (38) and preussochromone C (39) [103][104][105]. Two other compounds, 9S,11R(+)-ascosalitoxin (40) and vermelhotin (41), were also produced by endophytes from this plant [105,106].…”

“…In addition, the compounds (3S,4S)-4-hydroxymellein (9), (3S)-8-methylmellein (10), and the quinone derivatives 2-hydroxy-5-methoxy-3-methylcyclohexa-2,5-diene-1,4-dione (47), 4S,5S,6S-4hydroxy-3-methoxy-5-methyl-5,6-epoxycyclohex-2-en-1-one (48), and 4R,5R-dihydroxy-3-methoxy-5methylcyclohexen-2-en-1-one (49) were obtained ( Figure 6) [108]. Extracts of solid cultures of Sporormiella minimoides (Sporormiaceae), isolated as an endophytic fungus from leaves Hintonia latiflora collected in Mexico, yielded five polyketides, 3,6-dimethoxy-8methyl-1H,6Hbenzo[de]isochromene-1,9-dione (31), 3-hydroxy-1,6,10-trimethoxy-8-methyl-1H,3Hbenzo[de]isochromen-9-one (32), 5-hydroxy-2,7-dimethoxy-8-methylnaphthoquinone (33), minimoidiones A (34) and B (35), along with four known compounds: corymbiferone (36), ziganein (37), brocaenol B (38) and preussochromone C (39) [103][104][105]. Two other compounds, 9S,11R(+)ascosalitoxin (40) and vermelhotin (41), were also produced by endophytes from this plant [105,106].…”

“…The research on microorganisms associated with the Rubiaceae family, for biotechnological applications, led to the isolation of endophytic fungi [ 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 ] and the discovery of several bioactive metabolites [ 38 , 48 , 49 , 50 , 51 , 52 , 53 , 54 , 55 ]. The diversity of chemical structures observed for secondary metabolites synthesized by fungi isolated from Rubiaceae species showed a dynamic range of metabolites pathways used by these microorganisms.…”