Graphene-metallic nanocomposites as modifiers in electrochemical glucose biosensor transducers

Abstract

Graphene sheets and three different graphene-metallic nanocomposites including graphene-copper (graphene-Cu), graphene-nickel (graphene-Ni) and graphene-platinum (graphene-Pt) were prepared and characterized in the first place. Then the electrochemical performances of these nanocomposites were tested in glucose biosensor transducers, which were formed by combining these metallic nanocomposites with glucose oxidase enzyme and glassy carbon paste electrode (GCPE). This is the first work that includes the usage of these graphene-Me nanocomposites as a part of glucose biosensor transducer. Fabricated amperometric biosensors linear ranges were obtained as follow: For the plain graphene, the linear range was found in the concentration range between 50 μM and 800 μM with the RSD (n = 3 for 50 μM glucose) value of 12.86% and LOD value of 7.2 μM. For graphene-Pt modified glucose biosensor, the linear range was between 10 μM and 600 μM with the RSD (n = 3 for 50 μM glucose) value of 3.45% and LOD value of 3.06 μM. In the case of graphene-Ni modified glucose biosensor, the values were 25 μM to 600 μM with the RSD (n = 3 for 50 μM glucose) value of 8.76% and LOD value of 24.71 μM and for graphene-Cu modified glucose biosensor linear range was 25 μM to 400 μM with the RSD (n = 3 for 50 μM glucose) value of 3.93% and LOD value of 2.87 μM.

Electrochemical Sensors and Biosensors Based on Graphene Functionalized with Metal Oxide Nanostructures for Healthcare Applications

“…Amongst the three graphene‐metallic nano‐composites, the graphene‐copper modified electrode exhibited the lowest detection limit of 2.87 μM between the linear range 25–400 μM. Graphene‐nickel had the highest detection limit of 24.71 μM in the linear range of 250−600 μM …”

“…Graphene-nickel had the highest detection limit of 24.71 μM in the linear range of 250 À 600 μM. [128] Further, in recent years the application of graphene as a glucose biosensor has progressed significantly. Shanbhag and Prasad [129] proposed the fabrication of graphene oxide and reduced graphene oxide as a glucose sensor, and successfully applied it for monitoring glucose concentration in saliva.…”

Thin Films and Composites Based on Graphene for Electrochemical Detection of Biologically‐relevant Molecules

“…Altuntas et al . prepared three graphene materials with metallic nanostructures: graphene‐Ni, graphene‐Cu, and graphene‐Pt, which were used to immobilize GOD on glassy carbon paste electrodes. Using amperometry they observed that these hybrid composites promote electrocatalysis of glucose oxidation at +0.90 V ( νs .…”

Atık İnsan Saçının Grafenoksit Olarak Değerlendirilmesi ve Bazı Karakteristik Özelliklerinin İncelenmesi

“…Here, the first step of graphene oxide synthesis is very important for graphene to exhibit the desired properties (conductivity, mechanical, etc.) [13][14][15]. 106…”