Graphene foam field-effect transistor for ultra-sensitive label-free detection of ATP

Xu, Shicai; Zhang, Chao; Jiang, Shouzhen; Hu, Guodong; Li, Xiaoyue; Zou, Yan; Liu, Hanping; Li, Jun; Li, Zhenhua; Wang, Xiaoxin; Li, Mingzhen; Wang, Jihua

doi:10.1016/j.snb.2018.12.129

Cited by 54 publications

(29 citation statements)

References 60 publications

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“…The BioFET response to the target adsorption can further provide information on the energetics of the receptor-target binding, by applying models based on the Langmuir adsorption isotherm. This has been previously obtained both from real-time measurements through flow cells [41,42] and by static measurements such as in our case [26,27,39,43,44]. Within this context, and taking into account the offset observed when nonspecific adsorption occurs (∆V 0 ), the V th variation can be described by:…”

Section: Energetics Of the Adsorption Processmentioning

confidence: 93%

A Reliable BioFET Immunosensor for Detection of p53 Tumour Suppressor in Physiological-Like Environment

Baldacchini

Montanarella

Francioso

et al. 2020

Sensors

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The concentration of wild-type tumour suppressor p53wt in cells and blood has a clinical significance for early diagnosis of some types of cancer. We developed a disposable, label-free, field-effect transistor-based immunosensor (BioFET), able to detect p53wt in physiological buffer solutions, over a wide concentration range. Microfabricated, high-purity gold electrodes were used as single-use extended gates (EG), which avoid direct interaction between the transistor gate and the biological solution. Debye screening, which normally hampers target charge effect on the FET gate potential and, consequently, on the registered FET drain-source current, at physiological ionic strength, was overcome by incorporating a biomolecule-permeable polymer layer on the EG electrode surface. Determination of an unknown p53wt concentration was obtained by calibrating the variation of the FET threshold voltage versus the target molecule concentration in buffer solution, with a sensitivity of 1.5 ± 0.2 mV/decade. The BioFET specificity was assessed by control experiments with proteins that may unspecifically bind at the EG surface, while 100pM p53wt concentration was established as limit of detection. This work paves the way for fast and highly sensitive tools for p53wt detection in physiological fluids, which deserve much interest in early cancer diagnosis and prognosis.

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Section: Energetics Of the Adsorption Processmentioning

confidence: 93%

A Reliable BioFET Immunosensor for Detection of p53 Tumour Suppressor in Physiological-Like Environment

Baldacchini

Montanarella

Francioso

et al. 2020

Sensors

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“…Researchers from China invented a variant of GFET biosensors in which graphene foam was used as electrical channel to detect adenosine triphosphate (ATP). An extremely large surface area and ultra-high sensitivity possessed by porous/hollow structures of three-dimensional (3D) graphene foam produced an ATP biosensor with a limit of detection (LOD) of 0.5 pM in a wide linear range from 0.5 pM to 50 μM, which is several orders lower than previous reports and thus opens a door for trace sensing of ATP at the picomolar level in a biological system [63]. Another modification on the GFET structure comes from a research group in America who embedded an Al 2 O 3 layer (3 nm thickness) onto a reduced graphene oxide (rGO) channel by the atomic layer deposition (ALD) technique to prevent nonspecific binding of water or unwanted species onto the rGO surface.…”

Section: Evolution Of Nanotransducers For Fet-based Sensorsmentioning

confidence: 99%

Field-Effect Transistor Biosensors for Biomedical Applications: Recent Advances and Future Prospects

Chen

2019

Sensors

172

117

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During recent years, field-effect transistor biosensors (Bio-FET) for biomedical applications have experienced a robust development with evolutions in FET characteristics as well as modification of bio-receptor structures. This review initially provides contemplation on this progress by analyzing and summarizing remarkable studies on two aforementioned aspects. The former includes fabricating unprecedented nanostructures and employing novel materials for FET transducers whereas the latter primarily synthesizes compact molecules as bio-probes (antibody fragments and aptamers). Afterwards, a future perspective on research of FET-biosensors is also predicted depending on current situations as well as its great demand in clinical trials of disease diagnosis. From these points of view, FET-biosensors with infinite advantages are expected to continuously advance as one of the most promising tools for biomedical applications.

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“…Moreover, some studies have focused on the label-free detection of biological molecules [27][28][29][30][31]. As a representative label-free detection tool in recent years, field-effect transistors (FETs), which utilize the electrical response to target molecules, can realize simple and fast detection of biomolecules [32][33][34]. In the FET-based biosensor, signals caused by the interactions between biomolecules on sensing interfaces are transformed into readable electrical signals and amplified with high sensitivity and good specificity [35].…”

Section: Introductionmentioning

confidence: 99%

Ultrasensitive Detection of COVID-19 Causative Virus (SARS-CoV-2) Spike Protein Using Laser Induced Graphene Field-Effect Transistor

et al. 2021

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COVID-19 is a highly contagious human infectious disease caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), and the war with the virus is still underway. Since no specific drugs have been made available yet and there is an imbalance between supply and demand for vaccines, early diagnosis and isolation are essential to control the outbreak. Current nucleic acid testing methods require high sample quality and laboratory conditions, which cannot meet flexible applications. Here, we report a laser-induced graphene field-effect transistor (LIG-FET) for detecting SARS-CoV-2. The FET was manufactured by different reduction degree LIG, with an oyster reef-like porous graphene channel to enrich the binding point between the virus protein and sensing area. After immobilizing specific antibodies in the channel, the FET can detect the SARS-CoV-2 spike protein in 15 min at a concentration of 1 pg/mL in phosphate-buffered saline (PBS) and 1 ng/mL in human serum. In addition, the sensor shows great specificity to the spike protein of SARS-CoV-2. Our sensors can realize fast production for COVID-19 rapid testing, as each LIG-FET can be fabricated by a laser platform in seconds. It is the first time that LIG has realized a virus sensing FET without any sample pretreatment or labeling, which paves the way for low-cost and rapid detection of COVID-19.

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Graphene foam field-effect transistor for ultra-sensitive label-free detection of ATP

Cited by 54 publications

References 60 publications

A Reliable BioFET Immunosensor for Detection of p53 Tumour Suppressor in Physiological-Like Environment

A Reliable BioFET Immunosensor for Detection of p53 Tumour Suppressor in Physiological-Like Environment

Field-Effect Transistor Biosensors for Biomedical Applications: Recent Advances and Future Prospects

Ultrasensitive Detection of COVID-19 Causative Virus (SARS-CoV-2) Spike Protein Using Laser Induced Graphene Field-Effect Transistor

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