Graphene and tricobalt tetraoxide nanoparticles based biosensor for electrochemical glutamate sensing

Abstract

An amperometric biosensor based on tricobalt tetraoxide nanoparticles (CoO), graphene (GR), and chitosan (CS) nanocomposite modified glassy carbon electrode (GCE) for sensitive determination of glutamate was fabricated. Scanning electron microscopy was implemented to characterize morphology of the nanocomposite. The biosensor showed optimum response within 25 s at pH 7.5 and 37 °C, at +0.70 V. The linear working range of biosensor for glutamate was from 4.0 × 10 to 6.0 × 10 M with a detection limit of 2.0 × 10 M and sensitivity of 0.73 μA/mM or 7.37 μA/mMcm. The relatively low Michaelis-Menten constant (1.09 mM) suggested enhanced enzyme affinity to glutamate. The glutamate biosensor lost 45% of its initial activity after three weeks.

In Vivo Glutamate Sensing inside the Mouse Brain with Perovskite Nickelate–Nafion Heterostructures

“…In recent years, several candidates for amperometric glutamate biosensors have been developed such as noble metals ( e.g ., Pt, Au, and Pd , ), glassy carbon, carbon fiber or carbon nanotubes (CNTs , ), polymers, , binary metal oxides ( e.g ., TiO 2 and CeO 2 , ), and perovskite oxides ( e.g ., titanates). , Additionally, several studies have been presented for implantation of glutamate sensors into the brain matter for real-time recording. ,,, However, improving both response time scale and detection limit simultaneously motivates the discovery of new platforms for sensing. We report such a biosensor using a cross-linking immobilizing method with Nafion-coated perovskite nickelate thin films.…”

“…Such Nafion-coated structure has been reported to provide enhanced selectivity for accurate measurements. 31,32 In recent years, several candidates for amperometric glutamate biosensors have been developed such as noble metals (e.g., Pt, Au, and Pd 33,34 ), glassy carbon, carbon fiber or carbon nanotubes (CNTs 35,36 ), polymers, 37,38 binary metal oxides (e.g., TiO 2 and CeO 2 39,40 ), and perovskite oxides (e.g., titanates). 23,41 Additionally, several studies have been presented for implantation of glutamate sensors into the brain matter for real-time recording.…”

Electrochemical detection of HIV-1 by nanomaterials

“…Various electrochemical biosensors design and fabricated for detection of targets (Anik et al 2016, Dalkıran et al 2016, Erdem et al 2014. There are several electrochemical detection methods such as:…”

A Highly Sensitive Amperometric Glutamate Oxidase Microbiosensor Based on a Reduced Graphene Oxide/Prussian Blue Nanocube/Gold Nanoparticle Composite Film-Modified Pt Electrode

“…Glutamate is oxidized by glutamate oxidase to generate hydrogen peroxide, and then hydrogen peroxide is oxidized at a specific potential, generating hydrogen ions and releasing electrons (Figure 1G). According to the following Randles-Sevcik equation [43], the properties of both the electrode and the analyte will affect the current generated by the oxidation reaction.…”