Abstract:AIDS is one of the global pandemic diseases that results from infection by HIV and was estimated 34.2 million people infected in 2011 by this virus. The investigators had previously shown that by early antiretroviral treatment, the risk of AIDS/HIV-related illness and transmission reduced significantly. Nanomaterials could be applied to improving the ability and sensitivity of sensors to detect serum biomarkers with low-sample volume. Moreover, results can be obtained faster. In this paper, we present a review… Show more
With the rise of zoonotic diseases in recent years, there is an urgent need for improved and more accessible screening and diagnostic methods to mitigate future outbreaks. The recent COVID-19 pandemic revealed an over-reliance on RT-PCR, a slow, costly and lab-based method for diagnostics. To better manage the pandemic, a high-throughput, rapid point-of-care device is needed for early detection and isolation of patients. Electrochemical biosensors offer a promising solution, as they can be used to perform on-site tests without the need for centralized labs, producing high-throughput and accurate measurements compared to rapid test kits. In this work, we detail important considerations for the use of electrochemical biosensors for the detection of respiratory viruses. Methods of enhancing signal outputs via amplification of the analyte, biorecognition of elements and modification of the transducer are also explained. The use of portable potentiostats and microfluidics chambers that create a miniature lab are also discussed in detail as an alternative to centralized laboratory settings. The state-of-the-art usage of portable potentiostats for detection of viruses is also elaborated and categorized according to detection technique: amperometry, voltammetry and electrochemical impedance spectroscopy. In terms of integration with microfluidics, RT-LAMP is identified as the preferred method for DNA amplification virus detection. RT-LAMP methods have shorter turnaround times compared to RT-PCR and do not require thermal cycling. Current applications of RT-LAMP for virus detection are also elaborated upon.
With the rise of zoonotic diseases in recent years, there is an urgent need for improved and more accessible screening and diagnostic methods to mitigate future outbreaks. The recent COVID-19 pandemic revealed an over-reliance on RT-PCR, a slow, costly and lab-based method for diagnostics. To better manage the pandemic, a high-throughput, rapid point-of-care device is needed for early detection and isolation of patients. Electrochemical biosensors offer a promising solution, as they can be used to perform on-site tests without the need for centralized labs, producing high-throughput and accurate measurements compared to rapid test kits. In this work, we detail important considerations for the use of electrochemical biosensors for the detection of respiratory viruses. Methods of enhancing signal outputs via amplification of the analyte, biorecognition of elements and modification of the transducer are also explained. The use of portable potentiostats and microfluidics chambers that create a miniature lab are also discussed in detail as an alternative to centralized laboratory settings. The state-of-the-art usage of portable potentiostats for detection of viruses is also elaborated and categorized according to detection technique: amperometry, voltammetry and electrochemical impedance spectroscopy. In terms of integration with microfluidics, RT-LAMP is identified as the preferred method for DNA amplification virus detection. RT-LAMP methods have shorter turnaround times compared to RT-PCR and do not require thermal cycling. Current applications of RT-LAMP for virus detection are also elaborated upon.
“…The experimental setup has been characterized by a cost lower than $2 with a 1-hour total assay time. The preliminary results on spiked samples have demonstrated that capacitance spectroscopy allowed a more sensitive method than impedance spectroscopy [ 33 ]. In another effort to detect the HIV-1 virus, capsid protein p24 has been revealed in untreated human serum samples [ 71 ].…”
Section: Spes For Detection Of Virusesmentioning
confidence: 99%
“…The advancement in material science highlights the fundamental role of novel nanomaterials in enhancing specificity, stability, and sensitivity of portable diagnostics: metal nanoparticles, magnetic nanoparticles, carbonaceous-based nanomaterials, conductive polymers are only some of the enhancers that are generally exploited [ [23] , [24] , [25] , [26] , [27] , [28] , [29] , [30] , [31] , [32] ]. Thus, this paper aims to give a critical overview of the role of various nanomaterials, the manufacture methods for screen-printed electrodes, and the strategies for the forthcoming sustainable detection of Human Immunodeficiency Virus (HIV), Hepatitis B and C Viruses, Zika Virus, Dengue Virus and Sars-CoV-2 [ [33] , [34] , [35] , [36] , [37] ], Figure 1 . Fig.…”
There is a growing interest in the development of portable, cost-effective, and easy-to-use biosensors for the rapid detection of diseases caused by infectious viruses: COVID-19 pandemic has highlighted the central role of diagnostics in response to global outbreaks. Among all the existing technologies, screen-printed electrodes (SPEs) represent a valuable technology for the detection of various viral pathogens. During the last five years, various nanomaterials have been utilized to modify SPEs to achieve convincing effects on the analytical performances of portable SPE-based diagnostics. Herein we would like to provide the readers a comprehensive investigation about the recent combination between SPEs and various nanomaterials for detecting viral pathogens. Manufacturing methods and features advances are critically discussed in the context of early-stage detection of diseases caused by HIV-1, HBV, HCV, Zika, Dengue, and Sars-CoV-2. A detailed table is reported to easily guide readers toward the “right” choice depending on the virus of interest.
“…90 According to recent studies, it is possible to decrease the transmission up to 96% by early antiretroviral treatment so rapid and accurate point-of-care (POC) detection of HIV infection status is critical for protecting patients. 91 For FET fabrication, graphene was exfoliated on SiO 2 /Si substrate and then electron beam lithography and thermal evaporation techniques were applied to pattern the electrodes. Graphene was reacted with EDC and NHS to activate its carboxylic groups on the surface.…”
Section: Antibody-based Sensors For Viral Antigen and Particle Detectionmentioning
Infectious diseases commonly occur in contaminated water, food, and bodily fluids and spread rapidly, resulting in death of humans and animals worldwide. Among infectious agents, viruses pose a serious threat to public health and global economy because they are often difficult to detect and their infections are hard to treat. Since it is crucial to develop rapid, accurate, cost-effective, and in-situ methods for early detection viruses, a variety of sensors have been reported so far. This review provides an overview of the recent developments in electrochemical sensors and biosensors for detecting viruses and use of these sensors on environmental, clinical and food monitoring. Electrochemical biosensors for determining viruses are divided into four main groups including nucleic acid-based, antibody-based, aptamer-based and antigen-based electrochemical biosensors. Finally, the drawbacks and advantages of each type of sensors are identified and discussed.
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