Grapevine virus A-mediated gene silencing in Nicotiana benthamiana and Vitis vinifera

Mookkan, Muruganantham; Moskovitz, Yoni; Haviv, Sabrina; Horesh, Tamar; Fenigstein, Annie; Preez, Jacques Du; Stephan, D.; Burger, Johan T.; Mawassi, Munir

doi:10.1016/j.jviromet.2008.10.010

Cited by 70 publications

(54 citation statements)

References 30 publications

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“…The previous attempts to generate a virus vector for grapevine did not succeed in the consistent systemic expression of a reporter gene, likely due to the aberrant infection (38). By illuminating the entire pathway of virus reproduction and transport, vLR2-GFP provided an invaluable tool for the investigation of the viral colonization of the vines during the seasons and in response to environmental cues.…”

Section: Host Adaptation As a Key To Generating An Efficient Virus Vementioning

confidence: 99%

Virus-Derived Gene Expression and RNA Interference Vector for Grapevine

Kurth

Peremyslov

Prokhnevsky

et al. 2012

J Virol

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bThe improvement of the agricultural and wine-making qualities of the grapevine (Vitis vinifera) is hampered by adherence to traditional varieties, the recalcitrance of this plant to genetic modifications, and public resistance to genetically modified organism (GMO) technologies. To address these challenges, we developed an RNA virus-based vector for the introduction of desired traits into grapevine without heritable modifications to the genome. This vector expresses recombinant proteins in the phloem tissue that is involved in sugar transport throughout the plant, from leaves to roots to berries. Furthermore, the vector provides a powerful RNA interference (RNAi) capability of regulating the expression of endogenous genes via virus-induced gene-silencing (VIGS) technology. Additional advantages of this vector include superb genetic capacity and stability, as well as the swiftness of technology implementation. The most significant applications of the viral vector include functional genomics of the grapevine and disease control via RNAi-enabled vaccination against pathogens or invertebrate pests.

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Section: Host Adaptation As a Key To Generating An Efficient Virus Vementioning

confidence: 99%

Virus-Derived Gene Expression and RNA Interference Vector for Grapevine

Kurth

Peremyslov

Prokhnevsky

et al. 2012

J Virol

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“…Additionally, this technique has enabled the introduction of virus-derived vectors into several V. vinifera cultivars [96,97]. Agroinoculation, first developed as a simple tool to study plantvirus interactions, is a popular method of choice for functional gene analysis of viral genomes.…”

Section: Agroinoculationmentioning

confidence: 99%

Development and Use of Biotechnology Tools for Grape Functional Analysis

Chialva¹,

Eichler²,

Muñoz³

et al. 2016

Grape and Wine Biotechnology

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The aim of this chapter is to provide a description of the latest scientific advances in the field of gene functional analysis in grapevine. It provides general information about the studies conducted during the past decade to understand the natural variation of this plant and how this information has been exploited for the understanding of traits of interest. Likewise, it is exposed how the use of biotechnology tools have helped to characterize the mechanisms of gene expression and its regulation, as well as the subcellular localization of proteins and their interactions with other molecules. Finally, an approximation to the new technologies of gene editing and their potential application in the functional study of grapevine has been carried out.

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“…This vector showed characteristic phloem-associated expression in inoculated grapevines involving the roots, stems, petioles, leaves and berries. A grapevine-A vector was also shown to induce inhibitory RNA (RNAi) of model genes in micropropogated grapevines [45]. These vectors for use in expression and RNAi-based functional genomics studies open new non-transgenic strategies for researchers in woody plants.…”

Section: Independent-virus Vectorsmentioning

confidence: 99%