Development and Use of Biotechnology Tools for Grape Functional Analysis

Chialva, Constanza; Eichler, Estefanía; Muñoz, Claudio; DiegoLijavetzky,

doi:10.5772/64915

Cited by 3 publications

(1 citation statement)

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“…To improve Agrobacterium-mediated transformation efficiency of dedifferentiated V. vinifera cv Monastrell cells, Chu et al (2016) used the Sonication-Assisted Agrobacteriummediated Transformation (SAAT) method that was previously developed for soybean transformation (Trick and Finer, 1997). This method significantly increases transformation efficiency (Chialva et al, 2016).…”

Section: Agrobacterium-derived Technologiesmentioning

confidence: 99%

The state-of-the-art of grapevine biotechnology and new breeding technologies (NBTS)

et al. 2019

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Context of the review:The manipulation of the genetic basis controlling grapevine adaptation and phenotypic plasticity can be performed either by classical genetics or biotechnologies. In the last 15 years, considerable knowledge has accumulated about the grapevine genome as well as the mechanisms involved in the interaction of the vine with the environment, pests and diseases. Despite the difficulties associated with genetic mapping in this species (allele diversity, chimerism, long generation intervals…), several major QTLs (quantitative trait loci) controlling important vegetative or reproductive traits have been identified. Considering the huge genotypic and phenotypic diversities existing in Vitis, breeding offers a substantial range of options to improve the performances of cultivars. However, even if marker-assisted selection was largely developed to shorten breeding programs, the selection of improved cultivars, whether for agronomic traits or disease tolerances, is still long and uncertain. Moreover, breeding by crossing does not preserve cultivar genetic background, when the wine industry and market are still based on varietal wines. Significance of the review: In grapevine, pioneering biotechnologies were set up in the 1960s to propagate and/or clean the material from micro-organisms. In the 1990s, the basis of genetic engineering was primary established through biolistic or Agrobacterium with several derived technologies refined in the last 10 years. The latest advance is represented by a group of technologies based on genome editing which allows a much more precise modification of the genome. These technologies, so-called NBTs (new breeding technologies), which theoretically do not deconstruct the phenotype of existing cultivars, could be potentially better accepted by the wine industry and consumers than previous GMO (genetically modified organism) approaches. This paper reviews the current state-ofthe-art of the biotechnologies available for grapevine genome manipulation and future prospects for genetic improvement.

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Section: Agrobacterium-derived Technologiesmentioning

confidence: 99%