Granulosicoccus coccoides sp. nov., isolated from leaves of seagrass (Zostera marina)

Abstract

A non-pigmented, motile, Gram-negative bacterium, strain Z 271 T , was isolated from the surface of leaves of the seagrass Zostera marina which was collected in Troitza Bay (Sea of Japan, Pacific Ocean). The new isolate grew between 5 6C and 28 6C and was slightly halophilic, tolerating environments containing up to 5 % (w/v) NaCl. Strain Z 271T was able to degrade Tweens 20, 40and 80 and partially degrade gelatin, but was unable to degrade casein. Phosphatidylethanolamine (36.9 %) and phosphatidylglycerol (63.1 %) were the predominant phospholipids. The genus Granulosicoccus, with the single species Granulosicoccus antarcticus, was described in 2007 as a member of the newly proposed family Granulosicoccaceae (Lee et al., 2007) of the order Chromatiales (Imhoff, 2005).In this study, we report the characterization of a novel bacterium of the genus Granulosicoccus, isolated in July 1998 from leaves of the seagrass Zostera marina. The seagrass was collected in Troitza Bay of the Gulf of Peter the Great (Kurilenko et al., 2007). A strain, Z 271 T , was isolated from an individual colony grown for 7 days at 28 u C on a medium (designated 'Medium A') containing: The following physiological and biochemical properties were determined by methods described by Smibert & Krieg (1994): oxidation/fermentation of glucose, denitrification (Azegami et al., 1987), oxidase and catalase activity, gelatin liquefaction, arginine dihydrolase, lysine decarboxylase, ornithine decarboxylase, sodium requirement [0, 0.5, 1, 3, 5, 8, 10, 12 % (w/v) NaCl], indole and H 2 S production and the ability to hydrolyse starch, Tween 80 and casein. The growth of the novel strain at different temperatures was determined in MB and on plates of Medium A after 24-72 h at 2, 4, 6, 29, 30, 35 and 37 u C. Biolog GN test kits were also used to examine the primary oxidation of 95 carbon compounds as described elsewhere (Ivanova et al.,Abbreviation: FAMEs, fatty acid methyl esters.The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain Z 271 T is FJ535355.Transmission electron micrographs of cells of strain Z 271 T and an extended phylogenetic tree are available as supplementary figures with the online version of this paper. A supplementary table detailing the cellular fatty acid composition of the novel strain is also available.

Microbial community structure of Arctic multiyear sea ice and surface seawater by 454 sequencing of the 16S RNA gene

“…Other newly observed clades are likely to be of marine origin, including the genera Persicivirga, Ulvibacter, Granulosicoccus and Lewinella. Representatives from the first three clades have characteristics that suggest an association with marine algae (Nedashkovskaya et al, 2004;Barbeyron et al, 2010;Kurilenko et al, 2010). The possibility that these clades were present in MYI because of sea ice diatoms warrants further study.…”

Microbiome shifts associated with the introduction of wild Atlantic horseshoe crabs (Limulus polyphemus) into a touch-tank exhibit

“…Thiothrix is a common ectosymbiont of marine invertebrates (Temara et al, 1992; Brigmon and de Ridder 1998; Bauermeister et al, 2012). The genus Granulosicoccus was prevalent in the oral cavity samples of the wild horseshoe crabs and is present in the microbiomes of several other marine organisms, such as seagrass (Kurilenko et al, 2010), kelp (Bengtsson et al, 2012), and corals (van Bleijswijk et al, 2015). A genomic analysis of the type strain Granulosicoccus antarcticus IMCC3135T (= KCCM 42676T = NBRC 102684T) revealed the presence of several genes associated with sulfur cycling; for example, the genome contained a gene encoding for the enzyme dimethylsulfoniopropionate (DMSP) demethylase and several genes associated with sulfur oxidation (Kang et al, 2018).…”

A report of 39 unrecorded bacterial species in Korea, belonging to the Betaproteobacteria and Gammaproteobacteria

“…5 shows phylogenetic assignment of 13 strains into 13 species of the orders Enterobacteriales, Vibrionales, Aeromonadales, Oceanospirillales, Xanthomonadales, and Chromatiales. These strains belonged to Cedecea davisae (Bouvet and Grimont, 1986), Enterobacter ludwigii (Hoffmann et al, 2005), Pantoea vagans (Brady et al, 2009), and Gibbsiella quercinecans (Brady et al, 2010) of the family Enterobacteriaceae, Vibrio tapetis (Borrego et al, 1996) and Enterovibrio norvegi- cus (Thompson et al, 2002) of the family Vibrionaceae, Aeromonas veronii (Hickman-Brenner et al, 1987) of the family Aeromonadaceae, Chromohalobacter canadensis (Arahal et al, 2001) and Kushneria indalinina (Sánchez-porro et al, 2009) of the family Halomonadaceae, Neptunomonas concharum (lee et al, 2012) of the family Oceanospirillaceae, Lysobacter enzymogenes (Christensen and Cook, 1978) and Rhodanobacter lindaniclasticus (Nalin et al, 1999) of the family Xanthomonadaceae, and Granulosicoccus coccoides (Kurilenko et al, 2010) of the family Granulosicoccaceae.…”