Granulocyte-colony stimulating factor may improve pregnancy outcome in patients with history of unexplained recurrent implantation failure: An RCT

Abstract

Background:Family of colony-stimulating factors (CSF) have an essential role on early cross talk between embryo and uterine endometrium.Objective:The aim of this study was to evaluate the effects of the single dose of Granulocyte-CSF (G-CSF) injection on clinical outcome of assisted reproductive technology cycle in patients with repeated implantation failures.Materials and Methods:This randomized control trial study was performed on 52 infertile women who referred to the clinic with the history of more than three previous In vitro fertilization/Intracytoplasmic sperm injection-embryo transfer failures. All patients were stimulated with standard long protocol. All embryos were transferred on day five in blastocyst stage in both groups. The treated group received 300 µg (0.5 ml) recombinant human G-CSF subcutaneously which was injected 30 min before blastocyst embryo transfer.Results:There was not statistically significant differences in abortion rate in G-CSF and control group (p=0.09). G-CSF treated group showed higher clinical pregnancy rate in comparison with control group (56.2% vs. 40.0%) but it was not statistically significant (p=0.09). Although live birth rate in G-CSF group was higher than control group (53.1% vs. 35.0%) but there wasn’t statistically significant difference in the overall live birth rate between the two groups (p=0.10). G-CSF group had a twin pregnancies while in control group there was no twin pregnancy.Conclusion:Our result demonstrates the possibility that pregnancy outcome is better in women with repeated unexplained In vitro fertilization failure who are treated with G-CSF.

New therapeutic protocol for improvement of endometrial receptivity (PRIMER) for patients with recurrent implantation failure (RIF) - A pilot study

“…This cytokine promotes endometrial immunomodulation and optimizes the interaction between the embryo and the endometrium. Studies have shown that its use is associated with higher pregnancy rates and lower miscarriage rates (Lédée et al ., 2013; Rahmati et al ., 2015; Arefi et al ., 2018).…”

Uterine Insulin Sensitivity Defects Induced Embryo Implantation Loss Associated with Mitochondrial Dysfunction‐Triggered Oxidative Stress

“…Implantation loss contributes greatly to the pregnancy loss for humans and mammalian animals [9,34]. To dissect the underlying mechanism of proliferative endometrial defects in implantation loss, a proteomic analysis of endometrium 12 Oxidative Medicine and Cellular Longevity from NRP and LRP sows was conducted in this study.…”

Approaches to Improve Endometrial Receptivity in Case of Repeated Implantation Failures

“…Different populations selected (infertile, RIF, cases with thin endometrium), different ways of administration (intrauterine, subcutaneously), different concentrations offered and different study endpoints made it difficult to extract a solid conclusion. So far 10 randomized controlled trials have been published ( Barad et al, 2014 ; Aleyasin et al, 2016 ; Davari-Tanha et al, 2016 ; Eftekhar et al,2016a,b ; Sarvi et al, 2017 ; Arefi et al, 2018 ; Bakirarar et al, 2018 ; Huang et al, 2020a ; Kalem et al, 2020 ), with conflicting results, followed by meta-analyses in a timely manner (presented in Table 5 ). The six meta-analyses published present rather supportive results ( Zhao et al, 2016 ; Kamath et al, 2017 ; Li et al, 2017a ; Hou et al, 2018 ; Jiang et al, 2020 ; Kamath et al, 2020 ).…”