Gram negative species associated with active destructive periodontal lesions

Abstract

Apical subgingival plaque samples were taken from 19 subjects exhibiting active destructive periodontal disease. The predominant cultivable Gram negative species from 50 active sites were compared to 69 inactive sites of comparable pocket depth and attachment level loss. Active disease sites were chosen which showed a significant loss of attachment within a two-month interval. Proportions of Gram negative rods were higher in active periodontal disease sites than in inactive sites. Species which were found to be significantly elevated only in active sites were Bacteroides intermedius, "fusiform" Bacteroides, Actinobacillus actinomycetemcomitans and Wolinella recta. Fusobacterium nucleatum, Capnocytophaga gingivalis and Eikenella corrodens were found in significantly increased proportions in active sites of some subjects and inactive sites of others.

Proposal of Three Subspecies of Fusobacterium nucleatum Knorr 1922: Fusobacterium nucleatum subsp. nucleatum subsp. nov., comb. nov.; Fusobacterium nucleatum subsp. polymorphum subsp. nov., nom. rev., comb. nov.; and Fusobacterium nucleatum subsp. vincentii subsp. nov., nom. rev., comb. nov.

“…nucleatum is of considerable interest to oral microbiologists. It is the most frequently detected species in predominant cultivable studies of the microbiota of subgingival plaque, averaging 8 to 10% of the microbiota (5, 6,20). F. nucleaturn has been associated with lesions of destructive periodontal disease, but has also been found in high numbers in successfully treated sites and in healthy sites (5, 6,20).…”

A double-blind randomized clinical trial of subgingival minocycline for chronic periodontitis

“…Ebersole et al (22) reported that E. corrodens was associated with sites in periodontal pockets, whereas another study (23) demonstrated a weak association with chronic periodontitis, and a third study (24) showed that there was no significant difference between active and inactive sites in the distribution of E. corrodens. In 2002, Avila-Campos and Velasques-Mendez (25) evaluated the prevalence of putative periodontopathogens in periodontal patients and healthy subjects and found that E. corrodens and F. nucleatum were not significantly associated with periodontal disease.…”

Identification and Characterization of an Invasion Antigen B Gene from the Oral Pathogen Campylobacter rectus

“…Campylobacter rectus is a poorly described Gram-negative, oral bacterium that has been implicated as a cause of periodontitis [3][4][5]. Recent studies indicate that women with periodontitis are seven times more likely to experience preterm labor than their healthy counterparts [6,7].…”