A double-blind randomized clinical trial of subgingival minocycline for chronic periodontitis

Abstract

The purpose of this study was to evaluate the presence of A. actinomycetemcomitans, P. gingivalis, P. intermedia, E. corrodens and F. nucleatum in 30 subjects with chronic periodontitis treated by scaling and root planing (SRP) plus minocycline (test group) during 12 months with regular trimester maintenance care. Additionally, we evaluated whether the beneficial effects of the therapy on the microbial flora persisted for 24 months. The test group (n = 15) and the control group [SRP plus placebo (n = 15)] were randomly assigned. After SRP, subjects received minocycline or placebo at the baseline, and at 3, 6, and 9 months at all sites with a periodontal pocket depth (PD) of ≥ 6 mm. Moreover, two homologous teeth, initially PD ≥ 6 mm, were clinically and microbially monitored by PCR at the baseline, and at 3, 6, 9, 12 and 24 months. Differences in mean PD values between groups were analyzed by Student's t-test (P < 0.05). The results for bacterial frequencies showed no significant differences between groups (Fisher's Exact test, P < 0.05) or between timepoints (Friedman test, P < 0.05). We failed to detect any differences between groups related to the presence of target pathogens for 12 months. The effects of both therapies on the microbial flora did not persist for 24 months. The group without supportive periodontal therapy showed an improvement in the pattern of

Alcohol Consumption and Periodontitis: Quantification of Periodontal Pathogens and Cytokines

“…Clearly, biofilm is represented by a complex consortium of bacteria, including pathogenic species that can be detected in lower proportions among periodontally healthy individuals. That periodontal pathogens can be found in distinct clinic conditions demonstrates that these bacteria are members of the indigenous microbiota 20,27,28 . However, it should be considered that some species have been associated with the type and severity of periodontitis 7 .…”

“…That periodontal pathogens can be found in distinct clinic conditions demonstrates that these bacteria are members of the indigenous microbiota. 20,27,28 However, it should be considered that some species have been associated with the type and severity of periodontitis. 7 Quantitative analyses have helped to better understand the relationship between microbiota and periodontal status and differentiate colonization from infection, in addition to making more clear the influence of demographic and behavioral factors in this relationship.…”

Microbiological outcomes from different periodontal maintenance interventions: a systematic review

“…Among these studies, only four reported the microbiological outcomes by numeric results on tables. 25,26,27,28 Real-time PCR: Kolbe et al 27 showed a non-significant trend to decrease the quantities (amounts: log 10 ± SEM) of P. gingivalis (1.6 ± 1.4 to 1.0 ± 1.4) and T. forsythia (4.9 ± 3.6 to 4.7 ± 3.8) from PMP baseline to 6 months, respectively, and stability for A. actinomycetemcomitans (2.7 ± 3.1 to 2.7 ± 2.5). There were no differences over time in the percentage of sites harboring T. forsythia and A. actinomycetemcomitans, whereas a significant decrease in the frequency of P. gingivalis was observed.…”

Local Delivery of Minocycline Hydrochloride: A Therapeutic Paradigm in Periodontal Diseases

“…(0.25-8 μg/ml), Pseudomonas aeruginosa (64-128 μg/ml) and Candida genus between (0.25-16 μg/ml) [36]. Clinical trials on subgingival minocycline in chronic periodontitis subjects showed that the scaling followed by subgingival minocycline is effective in reducing periodontal pathogens in chronic periodontitis [37,38]. Also, the effect of minocycline on cell survivals in cell lines showed that cell survivals were not decreased when the cells were treated with minocycline at 10 M and this concentration is greater than the MIC 90 doses of most periodontopathogens [39].…”