Identification and Characterization of an Invasion Antigen B Gene from the Oral Pathogen Campylobacter rectus

LaGier, Michael J.; Threadgill, Deborah S.

doi:10.1007/s12088-013-0406-z

Cited by 6 publications

(5 citation statements)

References 42 publications

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“…Similarly to T. forsythia , C. rectus has also been demonstrated to produce a unique surface‐layer structure, with similarities in characteristics of this surface layer between C. rectus isolates from human and nonhuman primates ; however, minimal data are available regarding the potential functions of this surface layer in oral colonization of this microorganism. A report by LaGier & Threadgill did identify a gene product from C. rectus (CheY) that may function as a chemotaxis signal for spread of the microorganism and has been identified in the virulence of Campylobacter jejuni .…”

Section: Characteristics Of the Microbiome In The Oral Cavity And Othmentioning

confidence: 99%

“…LaGier & Threadgill identified an array of genes in C. rectus that were distinct from the medically important pathogen, Campylobacter fetus , with some only found in oral bacterial species. They further described the invasion antigen B (ciaB) that is needed for cell invasion by C. fetus . Because of the reported linkage between C. rectus and adverse birth outcomes, the interaction of this oral microorganism with human gingival fibroblasts in the presence of various pregnancy hormones (estradiol and progesterone) was examined.…”

Section: Evasinsmentioning

confidence: 99%

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The periodontal war: microbes and immunity

Ebersole¹,

Dawson²,

Emecen‐Huja³

et al. 2017

Periodontology 2000

143

112

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Maintenance of periodontal health or transition to a periodontal lesion reflects the continuous and ongoing battle between the vast microbial ecology in the oral cavity and the array of resident and emigrating inflammatory/immune cells in the periodontium. This war clearly signifies many 'battlefronts' representing the interface of the mucosal-surface cells with the dynamic biofilms composed of commensal and potential pathogenic species, as well as more recent knowledge demonstrating active invasion of cells and tissues of the periodontium leading to skirmishes in connective tissue, the locality of bone and even in the local vasculature. Research in the discipline has uncovered a concerted effort of the microbiome, using an array of survival strategies, to interact with other bacteria and host cells. These strategies aid in colonization by 'ambushing, infiltrating and outflanking' host cells and molecules, responding to local environmental changes (including booby traps for host biomolecules), communicating within and between genera and species that provide MASINT (Measurement and Signature Intelligence) to enhance sustained survival, sabotage the host inflammatory and immune responses and by potentially adopting a 'Fabian strategy' with a war of attrition and resulting disease manifestations. Additionally, much has been learned regarding the ever-increasing complexity of the host-response armamentarium at both cellular and molecular levels that is addressed in this review. Knowledge regarding how these systems fully interact requires both new laboratory and clinical tools, as well as sophisticated modeling of the networks that help maintain homeostasis and are dysregulated in disease. Finally, the triggers resulting in a 'coup de main' by the microbiome (exacerbation of disease) and the characteristics of susceptible hosts that can result in 'pyrrhic victories' with collateral damage to host tissues, the hallmark of periodontitis, remains unclear. While much has been learned, substantial gaps in our understanding of the 'parameters of this war' remain elusive toward fulfilling the Sun Tzu adage: 'If you know the enemy and know yourself, you need not fear the result of a hundred battles.'

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Section: Characteristics Of the Microbiome In The Oral Cavity And Othmentioning

confidence: 99%

Section: Evasinsmentioning

confidence: 99%

The periodontal war: microbes and immunity

Ebersole¹,

Dawson²,

Emecen‐Huja³

et al. 2017

Periodontology 2000

143

112

View full text Add to dashboard Cite

show abstract

“…Despite the limitation given by the small sample size, due to the rarity of the disease, the oral microbiota samples revealed an almost 30% higher bacterial load in WAS patients, including species strongly associated with periodontitis (Bale et al, 2017; Lagier & Threadgill, 2014), if compared to age matched healthy donors. In particular, we detected a high to very high bacterial load for P. gingivalis (bacterial load: absolute amount 629, relative amount 0.85%), and T. forsythia (bacterial load: absolute amount 650, relative amount 0.82%).…”

Section: Resultsmentioning

confidence: 97%

“…After the removal of supra‐gingival biofilm, a sample of the gingival sulcus microbiota was taken from a single site using sterile paper probes to detect the presence of the main six periodontal bacteria ( Aggregatibacter actinomycetemcomitans , Porphyromonas gingivalis , Tannerella forsythia , Treponema denticola , Fusobacterium nucleatum , Campylobacter rectus ) (Bale et al, 2017; Lagier & Threadgill, 2014). The site of the analysis was the second superior left deciduous molar or the first–second superior left permanent premolar.…”

Section: Methodsmentioning

confidence: 99%

Wiskott–Aldrich syndrome: Oral findings and microbiota in children and review of the literature

Lucchese

Cenciarelli

Manuelli

et al. 2022

Clinical & Exp Dental Res

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Objective Wiskott–Aldrich syndrome (WAS) is a rare X‐linked primary immunodeficiency, characterized by micro‐thrombocytopenia, recurrent infections, and eczema. This study aims to describe common oral manifestations and evaluate oral microbioma of WAS patients. Material and methods In this cohort study, 11 male WAS patients and 16 male healthy controls were evaluated in our Center between 2010 and 2018. Data about clinical history, oral examination, Gingival Index (GI) and Plaque Index (PI) were collected from both groups. Periodontal microbiological flora was evaluated on samples of the gingival sulcus. Results WAS subjects presented with premature loss of deciduous and permanent teeth, inclusions, eruption disturbance, and significantly worse GI and PI. They also showed a trend toward a higher total bacterial load. Fusobacterium nucleatum, reported to contribute to periodontitis onset, was the most prevalent bacteria, together with Porphyromonas gingivalis and Tannerella forsythia. Conclusions Our data suggest that WAS patients are at greater risk of alterations in the oral cavity. The statistically higher incidence of periodontitis and the trend to higher prevalence of potentially pathological bacterial species in our small cohort, that should be confirmed in future in a larger population, underline the importance of dentistry monitoring as part of the multidisciplinary management of WAS patients.

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“…A few studies have identified potential virulence factors in C. rectus , including toxin genes ( csxA and csxB; csxC and csxD ), a potential cell invasion gene ( ciaB ), and a surface array protein ( crsA gene) thought to play a role in avoiding the host immune system (Braun et al, 1999; LaGier and Threadgill, 2008; Wang et al, 2000; LaGier and Threadgill, 2014). Within the campylobacters, virulence factors have been best characterized from the gastrointestinal pathogen Campylobacter jejuni (Young et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Identification and Characterization of a Putative Chemotaxis Protein, CheY, from the Oral Pathogen C

LaGier¹,

Bilokopytov²,

Cockerill³

et al. 2014

The Internet Journal of Microbiology

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Campylobacter rectus is an understudied oral bacterium that contributes to periodontitis. Processes that contribute to the disease-causing capabilities of pathogens, such as chemotaxis, are largely unknown in C. rectus. The aim of this study was to better understand C. rectus chemotaxis, by examining the C. rectus genome for the presence of a cheY gene. CheY proteins play a part in chemotaxis by acting as two-component response regulators. Significantly, CheY proteins from several pathogens, including the related species Campylobacter jejuni, have been shown to contribute to bacterial virulence. Degenerate PCR, RT-PCR, sequence analyses, and structural modeling showed that C. rectus encodes a gene (Cr-CheY) which shares significant homology with previously characterized CheY proteins. Functional studies of a recombinant form of the protein supports a likely role of Cr-CheY in C. rectus chemotaxis. Cr-CheY is the first CheY characterized from the oral campylobacters.

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Identification and Characterization of an Invasion Antigen B Gene from the Oral Pathogen Campylobacter rectus

Cited by 6 publications

References 42 publications

The periodontal war: microbes and immunity

The periodontal war: microbes and immunity

Wiskott–Aldrich syndrome: Oral findings and microbiota in children and review of the literature

Identification and Characterization of a Putative Chemotaxis Protein, CheY, from the Oral Pathogen C

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