Gradient Boosted Decision Tree Classification of Endophthalmitis Versus Uveitis and Lymphoma from Aqueous and Vitreous IL-6 and IL-10 Levels

Kuo, David; Wei, Maggie; Armbrust, Karen R; Knickelbein, Jared E.; Yeung, Ian; Nussenblatt, Robert B.; Chan, Chi‐Chao; Sen, H. Nida

doi:10.1089/jop.2016.0132

Cited by 18 publications

(5 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Malignant B cells often express high levels of IL‐10, while inflammatory cells produce high levels of IL‐6. Therefore, an IL‐10/IL‐6 ratio greater than 1 has high specificity in favour of B‐lymphoma, which was the case for 8/12 of DLBCL patients in our cohort . The dilution of the sample may have contributed to the low positive cytokine outcome (58%) in this cohort, compared to a better rate in the literature (>90% of cases).…”

Section: Discussionmentioning

confidence: 51%

See 1 more Smart Citation

Cytological diagnosis of vitreoretinal lymphomas: A case series

et al. 2019

View full text Add to dashboard Cite

Objective To assess the cytological diagnosis and follow‐up of patients suffering from vitreoretinal lymphoma (VRL) diagnosed in our institution. Methods and Results From January 2010 to June 2017, we collected 15 patients with VRL. Twelve patients had diffuse large B‐cell lymphoma (DLBCL); of these, 11 had primary central nervous system (CNS) DLBCL, one had ocular localisation of follicular lymphoma, one had extranodal NK/T‐cell nasal type lymphoma and one had chronic lymphocytic leukaemia. The results of the cytological examination (cell morphology and immunocytochemistry) of the vitreous fluid were available for 9/15 VRL. The interleukin‐10/‐6 ratio was >1 in eight of 12 DLBCL. Molecular testing was useful in 6/15 cases (clonality evaluation or MYD88 L265P mutation testing). Eight out of 11 primary CNS DLBCL patients had CNS involvement, with 22‐month progression‐free survival. In our series, only two out of 11 CNS DLBCL patients died of disease after 2 and 5 years, respectively. Conclusions The short delay to assert the diagnosis of VRL could explain the quite good prognosis in our series, which highlights the need to consider a diagnosis of DLBCL as first step. The cytological features, as a reliable way to identify VRL, must always guide the choice of techniques for further investigations given the small amount of vitreous fluid available for analysis.

show abstract

Section: Discussionmentioning

confidence: 51%

“…The dilution of the sample may have contributed to the low positive cytokine outcome (58%) in this cohort, compared to a better rate in the literature (>90% of cases). However, IL can be variable in pathological aqueous or vitreous samples, regardless of the pathology, including endophthalmitis, uveitis or lymphoma …”

Section: Discussionmentioning

confidence: 99%

Cytological diagnosis of vitreoretinal lymphomas: A case series

et al. 2019

View full text Add to dashboard Cite

show abstract

“…Moreover, in animal model with endotoxininduced uveitis protective role of local IL-10 was confirmed [59,60]. Сorrelation between level of IL-10 to level of IL-6 allows to determine the diagnosis (uveitis or intraocular tumor) [61,62].…”

Section: Review and Discussionmentioning

confidence: 89%

Features of Clinical Manifestations, Free Radical, Coagulation and Aggregation Properties of Blood in Patients With Craniocerebral Trauma

Пінчук

Sylenko

et al. 2019

Wiad Lek

View full text Add to dashboard Cite

Introduction: In recent years in Ukraine, as in the entire world, there has been an increase in cases of domestic injuries with a raise in the number of patients with craniocerebral trauma and its consequences. The aim of our research was to study clinical symptoms, the state of free radical oxidation, aggregate and hemocoagulative properties of blood in patients with mild craniocerebral trauma. Materials and methods: We conducted comprehensive clinical and laboratory examination of 34 patients with mild craniocerebral trauma and 20 apparently healthy patients (the control group). The diagnosis was made under ICD-10. The verification of the diagnosis was based on data from clinical examination, neurological status of neuroimaging (computer tomography and magnetic resonance imaging). The study group included 24 men and 10 women aged from 19 to 40. In addition, 20 apparently healthy individuals of the same age were examined, who comprised the control group (14 men and 6 women). In the blood parameters of all patients, we examined free radical oxidation, hemostasis and aggregation properties of platelets. Results: The conducted study revealed clinical disturbances, changes in the parameters of free radical lipid oxidation, coagulation and microcirculatory hemostasis. Conclusions: All patients with mild craniocerebral trauma, develop disturbances in the form of symptoms of microorganic lesion of the central nervous system: convergence weakness in 100% of patients, Mann’s symptom (76.4%), disruption of the function of the cranial nerves (58.8%), signs of pyramidal insufficiency (68.4%), vestibular disorders (94.1%), autonomic vascular dysfunction (100%). Mild craniocerebral trauma is accompanied by the activation of free radical lipid oxidation processes, decreased activity of antioxidant enzymes, hypercoagulation, and increased aggregation properties of platelets.

show abstract

“…In addition, our previous study showed that rno-miR-30b-5p was upregulated in peripheral blood mononuclear cells in EAU rats; the predicted target genes are related to Becn1, Atg 12 and IL-10, IL21r, and TLR 4, which are involved in autophagy, immune signaling pathways [ 22 ]. It is well known that both IL-10 [ 33 ] and TLR4 [ 34 ] are involved in the development of uveitis. Therefore, we further explored the relationship between the expression levels of rno-miR-30b-5p and IL-10 and TLR4 and the development of uveitis.…”

Section: Discussionmentioning

confidence: 99%

Regulatory Role of rno-miR-30b-5p in IL-10 and Toll-like Receptor 4 Expressions of T Lymphocytes in Experimental Autoimmune Uveitis In Vitro

Sun

Guo

Liu

et al. 2018

Mediators of Inflammation

View full text Add to dashboard Cite

Uveitis is a serious eye disease that usually damages young adult's health. MicroRNAs (miRNAs) are a class of small noncoding RNAs which regulate messenger RNA (mRNA) expression. It is predicted that rno-miR-30b-5p can regulate the expressions of interleukin-10 (IL-10) and Toll-like receptor 4 (TLR4). In this study, the regulatory role of rno-miR-30b-5p in IL-10 and TLR4 gene expressions was validated using luciferase activity assay. Further, the inflammatory manifestation of the anterior segment and pathological examination of the eye were explored in experimental autoimmune uveitis (EAU) rats. Meanwhile, the levels of rno-miR-30b-5p in eye tissues, spleen, and lymph nodes were measured using quantitative PCR (Q-PCR). IL-10 and TLR4 in spleen and lymph nodes were further separately determined by using Q-PCR and Enzyme-Linked Immunosorbent Assay (ELISA). Moreover, rno-miR-30b-5p mimic and its inhibitor were separately transfected into purified T cells, and the levels of IL-10 and TLR4 were detected using PCR, flow cytometry, and ELISA techniques. Results indicate that rno-miR-30b-5p was downregulated in spleen, lymph nodes, and eye tissues whereas the expressions of IL-10 and TLR4 at mRNA and protein levels were upregulated. The levels of IL-10 and TLR4 were negatively correlated to rno-miR-30b-5p levels. The result of in vitro cell transfection experiment indicates that IL-10 and TLR4 expressions were inhibited at mRNA and protein levels after T cells incubated with rno-miR-30b-5p mimic. However, the IL-10 and TLR4 mRNA levels were upregulated in purified T cells from spleen and lymph nodes after treatment with miR-30b-5p antagonist. In addition, there was no evident change of IL-10 and TLR4 proteins in spleen and lymph node T cells between EAU control and negative treatment groups. Flow cytometry analysis revealed that rno-miR-30b-5p mimic could reduce the number of both IL-10 and TLR4 positive cells, whereas rno-miR-30b-5p inhibitor could increase the number of IL-10 and TLR4 positive cells. Our study demonstrates that rno-miR-30b-5p influences the development of uveitis by regulating the level of IL-10 and TLR4 positive cells, thereby playing a role in the pathogenesis of uveitis.

show abstract

Gradient Boosted Decision Tree Classification of Endophthalmitis Versus Uveitis and Lymphoma from Aqueous and Vitreous IL-6 and IL-10 Levels

Abstract: With limited sample size, gradient boosting can differentiate endophthalmitis from uveitis and lymphoma by IL-6 and IL-10 with high sensitivity and specificity; however, a larger cohort is needed for further validation.

Cited by 18 publications

References 18 publications

Cytological diagnosis of vitreoretinal lymphomas: A case series

Cytological diagnosis of vitreoretinal lymphomas: A case series

Features of Clinical Manifestations, Free Radical, Coagulation and Aggregation Properties of Blood in Patients With Craniocerebral Trauma

Regulatory Role of rno-miR-30b-5p in IL-10 and Toll-like Receptor 4 Expressions of T Lymphocytes in Experimental Autoimmune Uveitis In Vitro

Contact Info

Product

Resources

About