GOx@ZIF‐8(NiPd) Nanoflower: An Artificial Enzyme System for Tandem Catalysis

Abstract

We report a facile approach to prepare an artificial enzyme system for tandem catalysis. NiPd hollow nanoparticles and glucose oxidase (GOx) were simultaneously immobilized on the zeolitic imidazolate framework 8 (ZIF-8) via a co-precipitation method. The as-prepared GOx@ZIF-8(NiPd) nanoflower not only exhibited the peroxidase-like activity of NiPd hollow nanoparticles but also maintained the enzymatic activity of GOx. A colorimetric sensor for rapid detection of glucose was realized through the GOx@ZIF-8(NiPd) based multi-enzyme system. Moreover, the GOx@ZIF-8(NiPd) modified electrode showed good bioactivity of GOx and high electrocatalytic activity for the oxygen reduction reaction (ORR), which could also be used for electrochemical detection of glucose.

“Non‐Naked” Gold with Glucose Oxidase‐Like Activity: A Nanozyme for Tandem Catalysis

“…With the development of nanotechnology, nanozymes are widely used as substitutes of natural enzymes . The reported enzyme‐like activities include the peroxidase‐like activity, superoxide dismutase‐like activity, catalase‐like activity, and so on.…”

Hollow Mesoporous Metal‐Organic Framework Microdisks via a Solid Template‐Based Approach and Post‐Synthetic Wet‐Chemical Etching for Protein Loading

“…In addition to increasing pore size of MOFs, researchers are also trying to make large‐scale assemblies of MOFs to expand their applications . Such superstructure can introduce various species of MOFs and form manifold structures such as hollow, core‐shell and yolk‐shell structures, combined with their uniform and high porosity, which endows MOFs capability to import active agents such as drug molecules and functional nanomaterials whose products show great potentials in areas such as catalysis, drug loading, gas storage and sorption and sensing . Though preparation of MOFs with various species, controlled morphologies and diameters have been achieved, the assembling of MOFs into microscale structure remains a demanding challenge.…”

High‐Performance Integrated Enzyme Cascade Bioplatform Based on Protein–BiPt Nanochain@Graphene Oxide Hybrid Guided One‐Pot Self‐Assembly Strategy

“…However, the respond efficiency of enzyme cascade platform is severely limited by the nanozyme activity. Furthermore, similar to nature enzyme cascade system, the direct immobilization of enzyme into inorganic scaffold leads to activity loss of the enzyme and mass transfer limitations between nanozyme and nature enzyme within the scaffold, which influences the efficiency of the IAEC system . Thus, the development of high‐performance IAEC bioplatform based on nanozymes is of a great challenge.…”

“…Furthermore, similar to nature enzyme cascade system, the direct immobilization of enzyme into inorganic scaffold leads to activity loss of the enzyme and mass transfer limitations between nanozyme and nature enzyme within the scaffold, which influences the efficiency of the IAEC system. [14][15][16][17] Thus, the development of high-performance IAEC bioplatform based on nanozymes is of a great challenge. As an effective and facile approach, direct immobilization of nature enzyme on nanozyme surface provides new opportunities for facilitating next generation of high-performance IAEC bioplatform.…”