Gonadotropin Ratio Affects the In Vitro Growth of Rhesus Ovarian Preantral Follicles

Abstract

In vitro follicle growth (IVFG) strategy is critical in the fertility preservation of cancer survivors; however, its optimal protocol needs to be developed using primate models since the availability of human samples is limited. Only a few previous studies have reported the successful IVFG of rhesus monkey ovaries using low-dose follicle-stimulating hormone (FSH) (0.3 or 3 ng/mL) and long-term culture (up to 5 weeks) and it is still uncertain in regard to the optimal culture duration and effective dose of treated gonadotropins applicable to the IVFG of rhesus preantral follicles. Recently, we have reported that the FSH to luteinizing hormone (LH) ratio affects the in vitro growth of murine ovarian follicles. We aimed to investigate whether gonadotropin ratios affect the efficiency of rhesus follicular growth in vitro. Ovaries were collected from six necropsied rhesus macaques (4–9 years) and preantral follicles were retrieved and cultured for 14 days using 200 mIU/mL FSH. The characteristics of follicular growth were compared between the FSH:LH=1:1 (n=24) and FSH:LH=2:1 (n=24) groups. High concentration gonadotropin treatment shortened the duration required for in vitro maturation of rhesus preantral follicles. The FSH:LH=2:1 group showed a faster follicular growth and enabled the acquisition of mature oocytes, although the expression of growth differentiation factor (GDF)-9 and anti-Müllerian hormone (AMH) did not differ significantly between the two groups. Taken together, high dose gonadotropin treatment can shorten the duration of IVFG and the gonadotropin ratio is important in the IVFG of rhesus monkey ovaries.

Induction of multiple ovulation via modulation of angiotensin II receptors in in vitro ovarian follicle culture models

“…The methods used for in vitro culture of follicles have been taken from previous studies (Kim et al , , ,c). Non‐coated and alginate‐coated single and multiple follicles were placed in droplets (20–40 μl) of culture media on a culture dish (Falcon 353004, 60 × 15 mm; BD Biosciences, Irvine, CA, USA), one follicle or follicle cluster per droplet, 25 droplets per dish.…”

Reference values of hematological and biochemical parameters in young-adult cynomolgus monkey (Macaca fascicularis) and rhesus monkey (Macaca mulatta) anesthetized with ketamine hydrochloride

“…For example, rodents, who are evolutionary and genetically distant from humans, share only 64% genome identity with humans [1], while macaques and humans are 92% genetically similar [2]. The cynomolgus (Macaca fascicularis) and the rhesus macaque (Macaca mulatta) are the most widely used NHP models in various biomedical research fields, including genomic analysis [3] and neurodegenerative disease [4,5], as well as reproductive biomedical research [6][7][8].…”

Efficient Production of Murine Uterine Damage Model

“…To date, many studies used murine models [28]. Since rodents have different reproductive physiology to humans, it is necessary to expand to the animal models with similar reproductive physiology such as non-human primate [29,30]. Furthermore, uterine function is closely related to ovarian cellular physiology and hormonal regulation [31][32][33][34][35][36][37], therefore, follow-up of maintaining reproductive function after approach or post-hormone treatment should be considered.…”