Gold-binding peptide as a selective layer for electrochemical detection of SARS-CoV-2 antibodies

Braz, Beatriz A.; Santiani, Manuel Hospinal; Martins, Gustavo Celestino; Gogola, Jeferson L.; Valenga, Márcia Gabriela Pianaro; Beirão, Breno Castello Branco; Bergamini, Márcio F.; Marcolino‐Júnior, Luiz H.; Soccol, Vanete Thomaz; Soccol, Carlos Ricardo

doi:10.1016/j.talanta.2023.124348

Cited by 15 publications

(6 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The sensor achieved an operating range from 75 ng mL −1 to 15 µg mL −1 with a sensitivity of 1.059 µA dec −1 . [ 212 ] Phage display is a powerful technique for the isolation and identification of peptides that bind to a particular target, characterized by its simplicity, efficiency, lower cost of mass production and good tissue permeability. Peptides isolated by this technique could be useful for the fabrication of sensing systems.…”

Section: Applications Of Peptide‐based Bioelectronic Materials and De...mentioning

confidence: 99%

Rational Design of Bio‐Inspired Peptide Electronic Materials toward Bionanotechnology: Strategies and Applications

Zhao,

Liu,

Tong

et al. 2024

Adv Funct Materials

View full text Add to dashboard Cite

Biologically inspired peptide‐based materials, as novel charge transport materials, have gained increasing interest in bioelectronics due to their remarkable electrical properties and inherent biocompatibility. Extensive studies have shown that peptides can self‐assemble into a variety of hierarchical nanostructures with unique physical properties through supramolecular interactions. Therefore, peptide‐based materials hold great promise for applications in emerging electronic fields such as sensing, energy harvesting, energy storage, and electronic transmission. Herein, this work proposes a review article to summarize the rational design and research progress of peptide‐based materials and devices in bioelectronics. This work first introduces the design strategies and assembly mechanism for constructing high‐performance peptide‐based electronic materials and devices. In the following part, the applications of peptide‐based electronic materials and devices are systematically classified and discussed, including sensors, piezoelectric nanogenerators, electrodes, and semiconductors. Finally, the remaining challenges and future perspectives of peptide‐based bioelectronic materials and devices are presented. This work believes that this review will provide inspiration and guidance for the design and development of innovative peptide‐based smart materials in the field of bioelectronics.

show abstract

Section: Applications Of Peptide‐based Bioelectronic Materials and De...mentioning

confidence: 99%

Rational Design of Bio‐Inspired Peptide Electronic Materials toward Bionanotechnology: Strategies and Applications

Zhao,

Liu,

Tong

et al. 2024

Adv Funct Materials

View full text Add to dashboard Cite

show abstract

“…Over the past few years, electrochemical methods have been widely used in immunosensors due to their advantages, such as excellent specificity, good sensitivity and low cost [ 7 , 8 , 9 ]. Label-free electrochemical immunosensors in particular have attracted more attention, due to the ease involved with their operation, saving time and eliminating complex labeling processes when compared to sandwich-type immunosensors [ 10 , 11 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

A Novel Label-Free Electrochemical Immunosensor for the Detection of Thyroid Transcription Factor 1 Using Ribbon-like Tungsten Disulfide-Reduced Graphene Oxide Nanohybrids and Gold Nanoparticles

Wang,

Tang,

Zhou

et al. 2024

Molecules

View full text Add to dashboard Cite

Thyroid transcription factor 1 (TTF1) is an important cancer-related biomarker for clinical diagnosis, especially for carcinomas of lung and thyroid origin. Herein, a novel label-free electrochemical immunosensor was prepared for TTF1 detection based on nanohybrids of ribbon-like tungsten disulfide-reduced graphene oxide (WS2-rGO) and gold nanoparticles (AuNPs). The proposed immunosensor employed H2O2 as the electrochemical probe because of the excellent peroxidase-like activity of ribbon-like WS2-rGO. The introduction of AuNPs not only enhanced the electrocatalytic activity of the immunosensor, but also provided immobilization sites for binding TTF1 antibodies. The electrochemical signals can be greatly amplified due to their excellent electrochemical performance, which realized the sensitive determination of TTF1 with a wide linear range of 0.025–50 ng mL−1 and a lower detection limit of 0.016 ng mL−1 (S/N = 3). Moreover, the immunosensor exhibited high selectivity, good reproducibility, and robust stability, as well as the ability to detect TTF1 in human serum with satisfactory results. These observed properties of the immunosensor enhance its potential practicability in clinical applications. This method can also be used for the detection of other tumor biomarkers by using the corresponding antigen–antibody complex.

show abstract

“…Two crucial components of the peptide employed as a recognition site were present: one based on the viral receptor binding domain (RBD), which can recognize antibodies of the spike protein (Anti-S), and another capable of interacting with gold nanoparticles. [27] Commercial electrical devices have also been modified to detect SARS-CoV-2. [28] The field of study continues to expand due to the few studies and Novelty behind the form of sensing.…”

Section: Introductionmentioning

confidence: 99%

A Simplistic Label‐Free Electrochemical Immunosensing Approach for Rapid and Sensitive Detection of Anti‐SARS‐COV‐2 Nucleocapsid Antibodies

Kock,

Du Plooy,

Cloete

et al. 2024

ChemistrySelect

View full text Add to dashboard Cite

Rapid and precise detection of SARS‐CoV‐2 antibodies is paramount for effective outbreak monitoring and vaccine efficacy assessment. While existing approaches for antibody detection often rely on complex electrochemical immunosensing with nanomaterial functionalization targeting S‐protein antibodies, their limitations in sensitivity and complexity have hindered widespread application. Here, we present a simplistic immunosensing platform designed for the rapid, and precise detection of SARS‐CoV‐2 specific IgG and Nucleocapsid antibodies. Notably, this study marks only the second exploration of SARS‐CoV‐2 N‐protein antibody detection. The platform utilizes traditional self‐assembled monolayers to establish selective bio‐affinity between SARS‐CoV‐2 specific Nucleocapsid antibodies and a gold electrode functionalized with the N‐protein antigen. Interestingly, despite the absence of nanomaterial functionalization, the developed platform achieves sensitivity comparable to existing sensors across a wide detection range (0.025 to 1 ng/mL) with an impressive limit of detection (0.019 ng/mL). The simplicity of the approach, relying solely on immunocomplex reactions, underscores that effective binding efficiency may be achieved in the absence of complex functionalization and determines its affordability, specificity, and high sensitivity. By eliminating the need for additional functionalization steps, the platform offers a streamlined solution for SARS‐CoV‐2 antibody detection and demonstrates the possibility of N‐protein antibody detection as a promising avenue for widespread application in SARS‐CoV‐2 outbreak monitoring and vaccine efficacy assessment particularly in underdeveloped regions.

show abstract

Gold-binding peptide as a selective layer for electrochemical detection of SARS-CoV-2 antibodies

Cited by 15 publications

References 41 publications

Rational Design of Bio‐Inspired Peptide Electronic Materials toward Bionanotechnology: Strategies and Applications

Rational Design of Bio‐Inspired Peptide Electronic Materials toward Bionanotechnology: Strategies and Applications

A Novel Label-Free Electrochemical Immunosensor for the Detection of Thyroid Transcription Factor 1 Using Ribbon-like Tungsten Disulfide-Reduced Graphene Oxide Nanohybrids and Gold Nanoparticles

A Simplistic Label‐Free Electrochemical Immunosensing Approach for Rapid and Sensitive Detection of Anti‐SARS‐COV‐2 Nucleocapsid Antibodies

Contact Info

Product

Resources

About