Gold and silver nanoparticles‐based laser desorption/ionization mass spectrometry method for detection and quantification of carboxylic acids

Abstract

A comparison of ionization efficiency for gold and silver nanoparticles used as an active media of matrix-less laser desorption/ionization (LDI) mass spectrometry (MS) methods was made for carboxylic acids including fatty acids. The matrix-assisted laser desorption/ionization (MALDI)-type targets containing monoisotopic cationic 109 Ag nanoparticles (109 AgNPs) and Au nanoparticles (AuNPs) were used for rapid MS measurements of 10 carboxylic acids of different chemical properties. Carboxylic acids were directly quantified in experiments with 10 000-fold concentration change conditions ranging from 1 mg/ml to 100 ng/ml which equates to 1 μg to 100 pg of carboxylic acids per measurement spot.

Nanostructured Layer of Silver for Detection of Small Biomolecules in Surface-Assisted Laser Desorption Ionization Mass Spectrometry

“…Using nanostructure-initiator mass method spectrometry (NIMS) as an LDI technique, Reindl et al [31] achieved a LOD value for palmitic acid at a level of 0.1 pmol/spot, which is almost two times lower than the value obtained for the silver nanostructured layer (Table 1). Kołodziej et al [40] reported the limit of detection at 50.9 µM or 13,056 ng/mL using the SALDI technique with the application of chemically synthesized silver nanoparticles. This may indicate that the proposed nanostructured silver layer can be considered one of the most sensitive LDI techniques for the detection of palmitic acid.…”

Infrared pulsed fiber laser‐produced silver‐109‐nanoparticles for laser desorption/ionization mass spectrometry of amino acids

“…Monoisotopic silver‐109 nanoparticles ( 109 AgNPs) were found to be very efficient for cationization of various type of compounds, for example, amino acids, fatty acids, saccharides, or mould toxins 11–14 . In this article, we present quantification result of amino acids on steel plate covered with chemically pure silver‐109 nanoparticles produced by a new method with the use of 1064‐nm pulsed fiber laser with 2D galvanometer scanner.…”

“…[7][8][9][10] Monoisotopic silver-109 nanoparticles ( 109 AgNPs) were found to be very efficient for cationization of various type of compounds, for example, amino acids, fatty acids, saccharides, or mould toxins. [11][12][13][14] In this article, we present quantification result of amino acids on steel plate covered with chemically pure silver-109 nanoparticles produced by a new method with the use of 1064-nm pulsed fiber laser with 2D galvanometer scanner. We also compare manual laser desorption/ ionization mass spectrometry (LDI MS) and semiautomatic laser desorption/ionization mass spectrometry imaging (LDI MSI) in quantification of amino acids along with discussion of results and comparison with various surface-assisted laser desorption ionization (SALDI) and MALDI methods previously used in amino acids analysis.…”

Laser Ablation Synthesis in Solution and Nebulization of Silver-109 Nanoparticles for Mass Spectrometry and Mass Spectrometry Imaging

“…One of the most utilized laser mass spectrometry methods is matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS), which was developed by Tanaka et al in 1988 . It offers soft ionization potential, being therefore a powerful analytical tool for the analysis of ionic high-molecular-weight molecules, such as peptides, proteins, and DNA/RNA, − but also, it is useful for detection of some nonionic classes of chemical compounds such as lipids, etc. − However, MALDI has not been too often applied to detect low-molecular-weight (LMW) compounds (MW < 1000 Da), because MALDI matrices are low-molecular-weight organic acids and produce a variety of matrix-related ions during the desorption/ionization process, which complicates the spectrum and causes suppression of analyte peaks. − …”