Gm Typing, IgG Subclasses of Anti‐Rh(D) and Severity of Hemolytic Disease of the Newborn

Alie-Daran, Simone J.; Dugoujon, Jean‐Michel; Fournié, A

doi:10.1111/j.1423-0410.1992.tb01184.x

Cited by 17 publications

(10 citation statements)

References 5 publications

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“…Many qualitative studies have been reported which con clude that IgGl or IgG3 antibodies cause the most severe HDN [4,13,15,16], Other investigators claim that those an tibodies composed of both IgGl and IgG3 cause the most severe HDN [2,7]. Inherent to the IgGl plus IgG3 group is a confounding aspect -which subclass is most prominent?…”

Section: Discussionmentioning

confidence: 99%

A Quantitative Assay for Subclassing IgG Alloantibodies Implicated in Hemolytic Disease of the Newborn

Thomas¹,

Shirey²,

Kickler³

1995

Vox Sanguinis

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Traditionally, IgG subclassing has been performed using qualitative assays. Quantitation of IgG subclasses may have prognostic value in evaluating alloim- munized pregnancies. A quantitative enzyme-linked immunosorbent assay (ELISA) was implemented for measuring IgG subclasses of red blood cell (RBC) antibodies (AB) isolated by adsorption/elution from the sera of alloimmunized pregnant women. The assay is a sandwich enzyme immunoassay using monoclonal antibodies specific for the relevant IgG subclasses and anti-human IgG peroxidase conjugate to quantitate the amount of bound IgG. The sensitivities of the assay for IgG1, 2, 3, and 4, respectively, were 4,23,4 and 2 µg/l. The results for each subclass for a given AB were expressed as a percentage of the total. In a series of pregnant mothers with ABs: E (4), Fy^a (2), Jk^a (1) and S (1), the mean percentage ± 1SD of each subclass was; IgGl 61 ±34; IgG2 14±22; IgG3 18±28 and IgG4 4±17. IgGl or IgG3 accounted for greater than 50% of the AB subclass distribution in 5 cases that resulted in hemolytic disease of the newborn (HDN). Although only a small number of samples was studied, changes in the concentrations of IgGl or IgG3 during gestation suggest a correlation with the presence or absence of HDN. The ELISA may be used to quantitate the IgG subclasses of RBC ABs and may be valuable in predicting the severity of HDN in alloimmunized pregnancies.

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Section: Discussionmentioning

confidence: 99%

A Quantitative Assay for Subclassing IgG Alloantibodies Implicated in Hemolytic Disease of the Newborn

Thomas¹,

Shirey²,

Kickler³

1995

Vox Sanguinis

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show abstract

“…Claims have also been made that IgGl anti-D may play a greater role in HDN than does IgG3 anti-D [8,[10][11][12]. However, the quantitative composition of maternal sera as regards IgGl and IgG3 anti-D, and its correlation with HDN has not been reported previously.…”

Section: Introductionmentioning

confidence: 93%

Prediction of the Severity of Haemolytic Disease of the Newborn

Garner¹,

Gorick²,

Lai³

et al. 1995

Vox Sanguinis

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Sera containing anti-D, taken from 44 RhD-negative women with RhD-positive infants, were tested in antibody-dependent cellular cytotoxicity (ADCC) and monocyte monolayer assays (MMA) which used similar target and effector cell populations. In addition, the anti-D concentration was measured in the Auto Analyzer and the number of IgGl and IgG3 anti-D molecules bound to the target red cells was measured by flow cytometry. The results of the functional assays and AutoAnalyzer quantitation were examined for correlation with IgG subclass quantitation and all results were compared for their ability to predict the severity of haemolytic disease of the newborn (HDN). ADCC correctly predicted HDN in 39/44 (88.6%) cases, AutoAnalyzer quantitation in 35/44 (79.5%) and the MMA in 32/44 (72.7%). For all three assays, the number of correct predictions was highest when the maternal serum contained both IgGl and IgG3 anti-D. ADCC activity and HDN were correlated with the number of cell-bound IgGl molecules (r>0.58), but MMA activity was most closely correlated with the number of cellbound IgG3 molecules (r = 0.68). Hence the superior predictive value of ADCC is due to its ability to reflect the IgGl component of maternal anti-D, which has a better correlation than IgG3 anti-D with the severity of HDN.

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“…In our laboratory, we use anti-GM alloantibodies for IgG subclassing (5,6). We previously demonstrated a correlation between the severity of HDN and IgG1 subclasses (40%) versus IgG3 (13%).…”

Section: Introductionmentioning

confidence: 98%

“…GM allotypes are restricted to one subclass and located on the heavy chains of the IgG1: G1M(1,2,3,17), IgG2: G2M(23) and IgG3: G3M (5,6,10,11,13,14,15,16,21,24,26,27,28). Generally, the GM system is not fully explored, due to the reading difficulties in the classical method of agglutination on slides or microtiter plates and to the difficulty in finding good typing reagents for certain specificities.…”

Section: Introductionmentioning

confidence: 99%

Gel test assay for IgG subclass detection by GM typing: Application to hemolytic disease of the newborn

Alie-Daram¹,

Fournié

Dugoujon³

2000

J. Clin. Lab. Anal.

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Gm Typing, IgG Subclasses of Anti‐Rh(D) and Severity of Hemolytic Disease of the Newborn

Cited by 17 publications

References 5 publications

A Quantitative Assay for Subclassing IgG Alloantibodies Implicated in Hemolytic Disease of the Newborn

A Quantitative Assay for Subclassing IgG Alloantibodies Implicated in Hemolytic Disease of the Newborn

Prediction of the Severity of Haemolytic Disease of the Newborn

Gel test assay for IgG subclass detection by GM typing: Application to hemolytic disease of the newborn

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