Glycosylation of Polyphosphazene Nanofibrous Membrane by Click Chemistry for Protein Recognition

Abstract

A novel core/sheath glycosylated polyphosphazene nanofi brous membrane for use in protein recognition is fabricated via coaxial electrospinning process with alkyl polyphosphazene as the sheath and polyacrylonitrile (PAN) as the core, followed by introduction of saccharide residues to the surface of the polyphosphazene nanofi brous membrane using alkyne-azide "click" chemistry. A glucose/protein binding assay effectively demonstrates that this nanofi brous membrane can selectively recognize Con A while showing almost no binding with bovine serum albumin (BSA). Furthermore, the affi nity capability of proteins (BSA and Con A) for the glycosylated polyphosphazene surface is investigated quantitatively using a surface plasmon resonance (SPR) technique.

Cyclodextrin-grafted electrospun cellulose acetate nanofibers via “Click” reaction for removal of phenanthrene

“…In another related study, Lancuski et al developed carbohydrate-decorated PCL nanofibers for the specific protein adhesion by applying "click" reaction [37]. In one of the associated studies, Qian et al reported the introduction of saccharide residues to the surface of the polyphosphazene nanofibrous membrane using "click" chemistry [38]. Most of the studies mentioned above focus on the biomedical applications of nanofibers.…”

Preparation of Polyphosphazene Hydrogels for Enzyme Immobilization

“…Hexachlorocyclotriphosphazene (HCCP, Boyuan New Material & Technology Co. Ltd., Ningbo, China) was purified by recrystallization from heptane at a temperature of 60 °C, followed by two cycles of vacuum sublimation. Poly(dichlorophosphazene) (PDCP) was synthesized via thermally initiated ring-opening polymerization of HCCP in a sealed Pyrex tube at 250 °C [38]. Tetrahydrofuran (THF) was dried by refluxing over a Na/K alloy and distilling under nitrogen.…”

“…Thus, the probability of side reactions by chain cleavage is reduced. Thirdly, polyphosphazenes bear greater synthetic flexibility, and thus, more control over the chemical and physical properties of a material is possible [38]. This allows the design of many variations of the polymer for specific applications.…”

Adsorption and Activity of Lipase on Polyphosphazene-Modified Polypropylene Membrane Surface

“…This kind of synthetic flexibility endows polyphosphazenes with tunable physical and chemical properties. The tunable physicochemical and biocompatible properties of polyphosphazenes make them promising in different biotechnological applications, such as drug delivery, biosensors, scaffolding materials and controlled release [25][26][27]. Moreover, polyphosphazene-based materials have been reported to be efficient supports in enzyme immobilization practices.…”