Preparation of Polyphosphazene Hydrogels for  Enzyme Immobilization

Qian, Yue-Cheng; Chen, Pengcheng; He, Gang; Huang, Xiaojun; Xu, Zhi‐Kang

doi:10.3390/molecules19079850

Cited by 25 publications

(16 citation statements)

References 47 publications

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“…need to go through protection/deprotection procedures for their nucleophilic substitution on PDCP. Fortunately, an extensive library of PPZs has been developed, including derivatives substituted with alkene, alkyne, or vinyl groups, for example, allylamine, 2‐aminoethyl methacrylate, allyl glycinate, methacrylic acid, and propargylamine derivatives. These PPZs with alkene and alkyne side chains can be used as secondary precursors for free‐radical polymerization .…”

Section: Part I Background and Fundamental Properties Of Ppzmentioning

confidence: 99%

Polyphosphazenes for the delivery of biopharmaceuticals

Hsu

Csaba

Alexander

et al. 2019

J of Applied Polymer Sci

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Polyphosphazenes (PPZs) are a relatively new family of polymers based on a nitrogen–phosphorous backbone where organic side groups can be grafted. The synthetic route to PPZs is highly versatile such that it is possible to add many different functionalities that change completely the physicochemical and biological properties of the polymers. For instance, PPZs can be designed with a variety of organic side groups that render these materials biodegradable and highly biocompatible. Based on these positive features, PPZs have been explored for many biomedical applications including the design of numerous advanced drug delivery systems. In this area, PPZs have been particularly investigated as materials for the formulation of biopharmaceuticals of high added value. These include protein‐ and polynucleotide‐based medicines, applications where PPZ carriers have obtained very positive results in preclinical models. A further area of major interest for PPZs has been vaccination, where these materials have obtained excellent results in vivo as polymer adjuvants and have advanced to clinical evaluation. © 2019 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2020, 137, 48688.

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Section: Part I Background and Fundamental Properties Of Ppzmentioning

confidence: 99%

Polyphosphazenes for the delivery of biopharmaceuticals

Hsu

Csaba

Alexander

et al. 2019

J of Applied Polymer Sci

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“…[12] A pre-dried flask was loaded with 5.0 g (14.4 mmol) of hexachlorocyclotriphosphazene and aluminum chloride as a catalyst at 5-10 wt%. Subsequent nucleophilic substitution at the chlorine phosphorus centers [14] was performed by transferring the product supernatant into a pre-dried flask containing 50 mL of anhydrous THF, where TEA and allylamine (both 3 eq. After the polymerization had proceeded for 3 h, the reactor was cooled to 120°C, and 8 mL of diglyme were injected into the reactor to solubilize the crude product and to minimize cross-linking.…”

Section: Synthesis Of the Precursor Poly(allylamino-phosphazene)mentioning

confidence: 99%

Structure‐Optimized Interpolymer Polyphosphazene Complexes for Effective Gene Delivery against Glioblastoma

Hsu

Sánchez-Gómez

Gómez-Ibarlucea

et al. 2018

Advanced Therapeutics

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Safe and efficient gene delivery vectors will enhance the prospects for polynucleotide-based therapies. Herein a new approach toward structurally optimized gene vector design based on the preparation of clickable poly(allylamino-phosphazene)s that can be converted to several cationic and anionic derivatives via thiol-ene addition is described. Simultaneous co-incubation of alkylamine-and alkylcarboxylate-poly(phosphazenes) with polynucleotide generates binary polyelectrolyte nanoparticles. Screening of a series of these complexes for transfection in glioblastoma cells shows that the inclusion of 6-mercaptohexanoic acid substituted poly(phosphazene)s in the complexes results in six-fold and 19-fold higher luciferase expression in U87MG cells and GBM1 primary cells, respectively. This effect is attributed to the specific ionization properties of these materials that improved polyplex intracellular trafficking. Transfection in 3D-spheroid models and subcutaneous xenograft U87MG tumors confirms higher transgene expression for the binary cationic/anionic poly(phosphazene) complexes compared to the related polycation-pDNA complexes and to PEI-pDNA complexes. The data also indicate a notable capacity of the mixed complexes to deliver genes to the inner cores of tumor spheroids. Extension of this approach to siRNA delivery shows that the mixed poly(phosphazene) complexes can silence DYRK1A, a gene implicated in glioblastoma initiation and progression, reducing U87MG cell renewal in vitro and delaying tumor growth in vivo.

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“…34 In this study, a cationic clickable polyphosphazene was successfully synthesized by a convenient two-step approach combining nucleophilic substitution and quaternization without traditional protection/deprotection procedures. The corresponding ionic hydrogels were prepared by crosslinking the cationic polyphosphazene and poly(ethylene glycol) dithiol (dithiol PEG) under UV irradiation.…”

Section: Introductionmentioning

confidence: 99%

Click synthesis of ionic strength-responsive polyphosphazene hydrogel for reversible binding of enzymes

et al. 2015

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In this study, a chemically crosslinkable cationic polyphosphazene was synthesized and fabricated into ionic strength-responsive hydrogels for enzyme binding. This novel polyphosphazene was synthesized via the macromolecular substitution reaction of poly(dichlorophosphazene) with 2-dimethylaminoethylamine, followed by the quaternization to yield the allyl groups. Hydrogels were easily prepared via the thiol-ene click reaction between polyphosphazene and poly(ethylene glycol) dithiol (dithiol PEG) under UV radiation. The inner three-dimensional structure of the hydrogels was investigated by swelling experiments, mechanical property tests, and field emission-scanning electron microscopy. The resulting hydrogels exhibited sensitivity to the ionic strength of the surrounding environment. Lipase from Candida rugosa was selected as the model enzyme for the entrapment in these hydrogels, resulting in a maximum enzyme loading of 16.6 mg g À1 and activity retention as high as 61.6%. Furthermore, the cationic hydrogels were effectively used for reversible enzyme binding owing to the electrostatic interaction, regulated by the ionic strength.

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Preparation of Polyphosphazene Hydrogels for Enzyme Immobilization

Cited by 25 publications

References 47 publications

Polyphosphazenes for the delivery of biopharmaceuticals

Polyphosphazenes for the delivery of biopharmaceuticals

Structure‐Optimized Interpolymer Polyphosphazene Complexes for Effective Gene Delivery against Glioblastoma

Click synthesis of ionic strength-responsive polyphosphazene hydrogel for reversible binding of enzymes

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