Glycosyl flavones from Humulus japonicus suppress MMP-1 production via decreasing oxidative stress in UVB irradiated human dermal fibroblasts

Abstract

Exposure to Ultraviolet (UV) light induces photoaging of skin, leading to wrinkles and sunburn. The perennial herb Humulus japonicus, widely distributed in Asia, is known to have antiinflammatory, antimicrobial, and antioxidant effects. However, the physiological activities of isolated compounds from H. japonicus have rarely been investigated. This study focused on the isolation of active compounds from H. japonicus and the evaluation of their effects on photoaging in UVB-irradiated human fibroblast (Hs68) cells. When the extract and four fractions of H. japonicus were treated respectively in UVB-irradiated Hs68 cells to investigate anti-photoaging effects, the ethyl acetate (EtOAc) fraction showed the strongest inhibitory effect on MMP-1 secretion. From EtOAc fraction, we isolated luteolin-8-C-glucoside (1), apigenin-8-C-glucoside (2), and luteolin-7-O-glucoside (3). These compounds suppressed UVB-induced MMP-1 production by inhibiting the phosphorylation of the mitogen-activated protein kinases (MAPKs) and activator protein-1 (AP-1). When the antioxidant activity of the compounds were estimated by conducting western blot, calculating the bond dissociation energies of the O-H bond (BDE) at different grade, and measuring radical scavenging activity, we found luteolin-8-C-glucoside (1) showed the strongest activity on the suppression of UVB-induced photoaging. These results demonstrate the inhibitory effect of three flavone glycosides derived from H. japonicus on MMP-1 production, MAPK and AP-1 signaling, and oxidative stress; this could prove useful in suppressing UVB induced photoaging.

The Molecular Mechanism of Polyphenols with Anti-Aging Activity in Aged Human Dermal Fibroblasts

“…Similarly, UVB-damaged fibroblasts were improved by several polyphenols [ 26 , 27 , 28 , 29 , 30 , 31 , 32 ] ( Table 1 ). Baicalin, delphinidin, ellagic acid (EA), fisetin, isoorientin, genistein, and luteolin increased the expression levels of collagen I and III, whereas it decreased the expression levels of MMP-1 and MMP-3 [ 26 , 27 , 28 , 29 , 30 , 31 , 32 ]. Interestingly, baicalin had no difference in the normal fibroblasts without UVB irradiation [ 26 ].…”

Shedding a New Light on Skin Aging, Iron- and Redox-Homeostasis and Emerging Natural Antioxidants

“…Additionally, apigenin treatment of skin fibroblasts strongly promoted the elevation of AO-related proteins, Nrf2, and HO-1 in a dose-and time-dependent manner. The study further revealed that the extract suppressed the UVB-induced MMP-1 production by inhibiting the phosphorylation of the MAPKs and AP-1 [297]. Moreover, apigenin has the ability to restore the proper function of the skin (e.g., DNA repair and cell viability) in both UVAand UVB-irradiated human keratinocytes and dermal fibroblasts [298,299].…”

Network Pharmacology Integrated with Molecular Docking Explores the Mechanisms of Naringin against Osteoporotic Fracture by Regulating Oxidative Stress

“…Based on our study, naringin may be a promising antioxidant, which would help relieve oxidative stress and ameliorate OF. Moreover, numerous studies have demonstrated that the expression of core targets including TNF [ 57 ], CASP3 [ 58 ], ESR1 [ 59 ], MMP2 [ 60 ], MMP1 [ 61 ], PPARG [ 62 ], CDKN1A [ 63 ], ACE [ 64 ], etc., plays an important part in regulating oxidative stress. Therefore, we speculated that NG could regulate core targets' expressions and osteoclast differentiation by oxidative stress in OF patients, so as to anti-OF.…”