Glycolytic enzymes of fowl and turkey spermatozoa

Wishart, G. J.; Carver, Lisa

doi:10.1016/0305-0491(84)90404-8

Cited by 7 publications

(5 citation statements)

References 14 publications

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“…Two additional enzyme activities related to glycolysis were measured: HK and GAPDH. HK is the initial enzyme of glycolysis that phosphorylates glucose within the cytoplasm, and GAPDH is the rate-limiting glycolytic enzyme of fowl sperm [33]. However, in either case, no difference was observed between genotypes (Table 5).…”

Section: Discussioncontrasting

confidence: 49%

Reduced Glucose Transport in Sperm from Roosters (Gallus domesticus) with Heritable Subfertility1

McLean

Jones

Froman

1997

Biology of Reproduction

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Roosters homozygous for the rose comb allele (R/R) are subfertile. In previous research, these subfertile roosters were characterized by an in vitro sperm penetration assay as having limited sperm motility. The objectives in the present study were to characterize sperm motility by computer-assisted sperm motion analysis and to account for a mechanism underlying poor sperm motility. Percentages of motile sperm differed between subfertile males and fertile controls (r/r) by 29% (p < 0.001). The concentration of intracellular ATP in sperm form subfertile roosters was less than in that from fertile controls (p < 0.001). The genotypic difference is sperm motility, as measured with the sperm penetration assay, was maintained when ATP production was dependent on anaerobic glycolysis (p < 0.001). In this case, sperm were incubated with exogenous glucose and cyanide. Consequently, we could not attribute the genotypic difference in sperm mobility to mitochondrial respiration. In contrast, glucose transport, as measured by the uptake of [1,2-3H]-2-deoxy-D-glucose, was reduced in sperm from subfertile roosters (p < 0.001). Neither hexokinase nor glyceraldehyde-3-phosphate dehydrogenase activity differed between genotypes (p > 0.05). Likewise, lactate dehydrogenase activity did not differ between genotypes (p > 0.05). As evidenced by creatine kinase activity and dynein ATPase activity, neither the potential for energy transfer nor utilization within the axoneme differed between genotypes (p > 0.05). Therefore, we attribute the subfertility of roosters homozygous for the rose comb allele to decreased spermatozoal glucose transport.

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Section: Discussioncontrasting

confidence: 49%

Reduced Glucose Transport in Sperm from Roosters (Gallus domesticus) with Heritable Subfertility1

McLean

Jones

Froman

1997

Biology of Reproduction

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“…More generally, it has been reported that aerobic metabolism occurs in all avian species studied to date, but sperm from some species (e.g. the chicken but not the turkey) may also metabolise glucose under anaerobic conditions, at least in vitro (Wishart and Carver, 1984). As a consequence, energy substrates may be added to diluents in order to prolong sperm viability and activity.…”

Section: Storage Of Avian Sperm In a Liquid Statementioning

confidence: 99%

Specific features of in vivo and in vitro sperm storage in birds

Blesbois

Brillard²

2007

Animal

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This review focuses on some of the main features of sperm selection and storage in birds mainly on the basis of studies performed in poultry species, with emphasis on the initial selection of sperm at the female vagina level prior to migration towards the sperm storage tubules. Sperm originating from low-quality males or subjected to inappropriate in vitro storage conditions are rapidly discarded, resulting in impaired fertility in corresponding flocks. In the absence of accessible and appropriate technology for matching the 'storing' potential of sperm in the oviduct, conditions for prolonged sperm storage under a liquid (through the use of semen extenders) or a solid state (cryopreservation) have received only limited attention, despite their potential interest to facilitate male and female management in poultry flocks. Despite this, technology for shortterm liquid storage is currently used in turkeys, guinea fowl and muscovy ducks and also in progress in chickens. In addition, technology for cryopreservation of avian semen has become available for some species (chicken, goose) to facilitate the management of genetic resources, including the preservation of rare and economically important breeds.

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“…However, energy utilisation rates are slowed equivalently and energy values, as spermatozoal ATP content, remain the same at temperatures between 5 and 40°C . The maintenance of metabolic processes and energy values are important for spermatozoal survival throughout this temperature range: turkey spermatozoa, for example, have a considerably lower glycolytic capacity than fowl spermatozoa (Wishart and Carver, 1984) and have an absolute requirement for oxygen to maintain energy values and fertilising ability at both 40° (Wishart, 1982) and 5°C (Wishart, 1981). This basic finding has led to significant improvements in practical turkey semen storage (for example, Lake et al, 1984) which have been exploited commercially (Dobrescu and Hacking, 1984).…”

Section: Energy Metabolism Of Avian Spermatozoa In Vitromentioning

confidence: 99%

Physiological changes in fowl and turkey spermatozoa duringin vitrostorage

Wishart

1989

British Poultry Science

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1. Although early work on semen storage has been rather empirical in approach, only basic research can provide a framework of biological mechanisms from which improvements in the techniques of cryopreservation and liquid semen storage can progress logically. 2. A major drawback in this work has been the lack of adequate tests for quantitating and differentiating aspects of 'fertility'. 3. Basic research has now provided techniques for assessing: sperm fertilising ability in terms of numbers of fertile eggs; the efficiency of hens' oviducts at accepting and retaining spermatozoa, and sperm 'quality' as motility, metabolism and plasma membrane patency. 4. These techniques may be used for a more critical assessment of the effects of both cryopreservation and liquid semen storage on sperm function, although the integrity of sperm surface proteins may be a more sensitive variable which has yet to be measured. 5. Further improvements in sperm cryopreservation technology are best approached through an understanding of the fundamental cellular and molecular changes which take place during freezing; thus far little is known of such changes in avian spermatozoa. 6. The ideal milieu for maintaining spermatozoa in liquid semen storage should mimic the environment of the oviducal sperm storage tubules; elucidation of the factors involved in progressing steadily.

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Glycolytic enzymes of fowl and turkey spermatozoa

Cited by 7 publications

References 14 publications

Reduced Glucose Transport in Sperm from Roosters (Gallus domesticus) with Heritable Subfertility1

Reduced Glucose Transport in Sperm from Roosters (Gallus domesticus) with Heritable Subfertility1

Specific features of in vivo and in vitro sperm storage in birds

Physiological changes in fowl and turkey spermatozoa duringin vitrostorage

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