Specific features of in vivo and in vitro sperm storage in birds

Blesbois, Élisabeth; Brillard, Jean-Pierre

doi:10.1017/s175173110700081x

Cited by 95 publications

(60 citation statements)

References 76 publications

(83 reference statements)

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“…Eggs were collected and candled from day 2 to 9 post insemination for the first AI and day 2 to 23 post insemination for the second AI. This second AI was made in order to (1) confirm the first AI results and (2) to observe the coherence of the fertile period according to the reproductive profile of the species (28). Hatchability of the eggs was also measured and did not show added differences between male progeny results.…”

Section: Methodsmentioning

confidence: 98%

Intact Cell MALDI-TOF MS on Sperm: A Molecular Test For Male Fertility Diagnosis

Soler

Labas

Thélie

et al. 2016

Molecular & Cellular Proteomics

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Section: Methodsmentioning

confidence: 98%

Intact Cell MALDI-TOF MS on Sperm: A Molecular Test For Male Fertility Diagnosis

Soler

Labas

Thélie

et al. 2016

Molecular & Cellular Proteomics

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“…Spermatozoa are therefore the primary cell types preserved in most emerging genome resource banking projects . Since the fertilisation capacity of cryopreserved poultry sperm is dramatically lower than that of any domestic mammalian species (Long, 2006), fresh semen of high quality is required; the succession of thermal, osmotic and mechanical stresses suffered during cryopreservation (Blesbois and Brillard, 2007) renders low quality sperm ineffective. Since sperm quality variables and the capacity to survive cryopreservation show strong within-breed variability (Nishiyama, 1961;, in vitro semen evaluation for the selection of the best semen donors is necessary; only the most fertile roosters should be used as donors in genome resource banking projects.…”

Section: Introductionmentioning

confidence: 99%

Sperm variables as predictors of fertility in Black Castellana roosters: use in the selection of sperm donors for genome resource banking purposes

Santiago-Moreno

Castaño²,

Coloma³

et al. 2009

Span. j. agric. res.

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Semen was collected from 10 Black Castellana roosters and the classic sperm variables (ejaculate volume, sperm concentration and sperm motility) examined. In addition, the hypo-osmotic swelling test was used to investigate sperm cell membrane integrity, and acidic aniline blue staining used to screen for morphological abnormalities (including acrosome integrity) and to examine the condensation status of the chromatin. The latter was also examined by Gram staining. Large and small semen volumes were associated high and low sperm concentrations respectively (R 2 =0.04, P<0.05). The percentage of motile spermatozoa correlated strongly with the percentage of sperm cells showing an intact acrosome (R 2 =0.13, P<0.001) and with the percentage of morphologically normal spermatozoa (R 2 =0.04, P<0.05). The percentage of Gram positive spermatozoa was positively correlated with semen appearance (R 2 =0.12, P<0.05), sperm cell concentration (R 2 =0.13, P<0.05), and with the sperm motility variables studied (R 2 =0.14, P<0.05 for percentage mobility, and R 2 =0.12, P<0.05 for quality of movement). Only three of the 10 roosters, all with fertilisation potentials of 80-90%, were considered potential sperm donors for genome resource banking purposes. The remaining birds were all of low fertility (≤ 50%); in fact, some produced semen volumes too small to perform fertility tests. Semen volume and membrane integrity were found to be the best variables for predicting the fertilisation potential of rooster ejaculates.Additional key words: aniline blue, artificial insemination, fertility, Gram staining, semen quality ResumenVariables espermáticas predictoras de fertilidad en gallos de raza Negra Castellana; uso en la selección de donantes en bancos de recursos genéticos Se utilizó semen de 10 gallos de raza Negra Castellana, en el que se realizó un espermiograma clásico (volumen eyaculado, concentración espermática, motilidad). Además, se usó el test de endósmosis para evaluar la integridad de membrana plasmática, y la tinción de azul de anilina para analizar morfoanomalías (incluyendo la integridad del acrosoma) y el estatus de condensación de la cromatina, el cual también se evaluó mediante la tinción de Gram. Los resultados muestran que el volumen seminal estaba correlacionado con la concentración espermática (R 2 =0,04; P<0,05). El porcentaje de espermatozoides mótiles mostró una correlación con el porcentaje de espermatozoides con acrosomas intactos (R 2 =0,13; P<0,001) y con el porcentaje de espermatozoides con morfología normal (R 2 =0,04; P<0,05). El porcentaje de células espermáticas Gram positivas estuvo correlacionado con el aspecto seminal (R 2 =0,12; P<0,05), la concentración espermática (R 2 =0,13; P<0.05) y variables de motilidad (R 2 =0,14; P<0,05 y R 2 =0,12; P<0,05, para el porcentaje de células mótiles y calidad del movimiento, respectivamente). Sólo tres de los 10 gallos estudiados mostraron altas tasas de fertilidad del 80-90%, y fueron considerados como los únicos donantes potenciales de semen en bancos...

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“…fter the entry of sperm into a female genital tract before ovulation, sperm accumulate to a certain amount and are stored in the female sperm reservoir [1], which is a widespread phenomenon among numerous avian, reptilian and mammalian species [2][3][4]. The sperm reservoir then releases sperm gradually for successful fertilization after a single insemination, the period of which varies from hours to weeks in different species [2,[4][5][6].…”

mentioning

confidence: 99%

Genome-Wide Transcript Expression Analysis in the Uterovaginal Junction in Association with Fertile Period in Tsaiya Ducks

Huang

Cheng

Yang

et al. 2011

Journal of Reproduction and Development

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Abstract. We performed the first genome-wide expression analysis to compare the differences in gene expression in the female sperm reservoir of the duck reproductive tract between two groups with long and short fertile periods to identify factors that may be associated with the fertile period using an oligonucleotide microarray. RNA was extracted from the uterovaginal junction (UV junction) of the two groups. Affymetrix chips containing comprehensive coverage of 32773 transcripts were hybridized with biotin-labeled cRNA, and three biological repeats were performed. We identified 27 transcripts as being differentially regulated. Interestingly, by mapping the differentially expressed transcripts to annotated pathways, we found that Neuropeptide Y (NPY), the RNA expression of which was increased by 2.96-fold in the short-fertile-period group as compared with the long-fertile-period group in our experiment, has been shown to reduce blood flow and substance supply to local tissues. Enah/Vasp-like (EVL), the RNA expression of which was significantly increased by 1.77-fold in the short-fertile-period group as compared with the long-period group, has been demonstrated to be important in activated T-cells. In contrast, trafficking kinesin-binding protein 1 (TRAK1), the expression of which was increased by 2.33-fold in the long-period group as compared with its counterparts, has been suggested to inhibit precocious activation of sperm and prolong sperm life in the female sperm reservoir. The results of real-time PCR confirmed the data obtained by microarray analysis. Our study demonstrated that combining global gene expression investigation with annotated pathway resources contributes to the understanding of sperm life when sustained in the UV junction.Key words: Differentially expressed transcripts, Duck, Fertile period, Uterovaginal junction (J. Reprod. Dev. 57: [731][732][733][734][735][736] 2011) A fter the entry of sperm into a female genital tract before ovulation, sperm accumulate to a certain amount and are stored in the female sperm reservoir [1], which is a widespread phenomenon among numerous avian, reptilian and mammalian species [2][3][4]. The sperm reservoir then releases sperm gradually for successful fertilization after a single insemination, the period of which varies from hours to weeks in different species [2,[4][5][6]. This period is defined as the fertile period. A long fertile period means prolonged sperm survival and leads to a high fertilization rate [7].The process of sperm migration in the female genital tract is more than simply transportation from the position of insemination to the position of fertilization. Rather, it is a complex continuum: entry into the lower genital tract, defense against the tract environment and immunological response, retention in the storage reservoir, activation, taxiing of activated sperm towards oocytes [8], and phagocytosis of nonfertilizing sperm. During sperm storage in the reservoir of the female reproductive tract, there is a close interaction between sperm...

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Specific features of in vivo and in vitro sperm storage in birds

Cited by 95 publications

References 76 publications

Intact Cell MALDI-TOF MS on Sperm: A Molecular Test For Male Fertility Diagnosis

Intact Cell MALDI-TOF MS on Sperm: A Molecular Test For Male Fertility Diagnosis

Sperm variables as predictors of fertility in Black Castellana roosters: use in the selection of sperm donors for genome resource banking purposes

Genome-Wide Transcript Expression Analysis in the Uterovaginal Junction in Association with Fertile Period in Tsaiya Ducks

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