Intact Cell MALDI-TOF MS on Sperm: A Molecular Test For Male Fertility Diagnosis

Soler, Laura; Labas, Valérie; Thélie, Aurore; Grasseau, Isabelle; Teixeira-Gomes, Ana Paula; Blesbois, Élisabeth

doi:10.1074/mcp.m116.058289

Cited by 29 publications

(41 citation statements)

References 53 publications

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“…For example, MALDI–MS has been successfully used to build a proteomic mass spectra database of different honeys and their MFPs to identify their geographical origin . As another example of application, an experimental model of male chicken fertility was designed to perform on‐cell direct proteomic fingerprinting by MALDI profiling and demonstrated the capability of the GA classifier to build a predictive model to classify chicken sperm fertility …”

Section: Resultsmentioning

confidence: 99%

“…Molecular mass fingerprinting (MFP) or signatures of biological matrices by MALDI–MS profiling is indeed a thriving approach, enabling the rapid detection of peptide/protein that can provide comparative information regarding immunity in Drosophila , insects’ appendices, and even from complex biological matrices. MALDI‐MS based peptidomics/proteomics typing has found practical applications in fingerprinting honeys to determine specific molecular signatures that classify honeys according to their geographical origins or to establish a robust proteomics mass fingerprint of sterile sperm chicken for diagnosis purpose …”

Section: Introductionmentioning

confidence: 99%

“…MALDI-MS based peptidomics/proteomics typing has found practical applications in fingerprinting honeys to determine specific molecular signatures that classify honeys according to their geographical origins [37] or to establish a robust proteomics mass fingerprint of sterile sperm chicken for diagnosis purpose. [38] Relying on previously published studies, [33,[39][40][41] we first developed and validated an experimental model of challenged honey bees with Gram-positive and -negative bacteria (using notably a Serratia marcescens strain we have isolated from honey bees). Then, we assessed the usefulness of MALDI profiling to fingerprint the peptides/proteins within the molecular mass range 2-20 kDa from honey bee hemolymph.…”

Section: Introductionmentioning

confidence: 99%

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MALDI–MS Profiling to Address Honey Bee Health Status under Bacterial Challenge through Computational Modeling

et al. 2019

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Honey bees play a critical role in the maintenance of plant biodiversity and sustainability of food webs. In the past few decades, bees have been subjected to biotic and abiotic threats causing various colony disorders. Therefore, monitoring solutions to help beekeepers to improve bee health are necessary. Matrix‐assisted laser desorption ionization–mass spectrometry (MALDI–MS) profiling has emerged within this decade as a powerful tool to identify in routine micro‐organisms and is currently used in real‐time clinical diagnosis. MALDI BeeTyping is developed to monitor significant hemolymph molecular changes in honey bees upon infection with a series of entomopathogenic Gram‐positive and ‐negative bacteria. A Serratia marcescens strain isolated from one naturally infected honey bee collected from the field is also considered. A series of hemolymph molecular mass fingerprints is individually recorded and to the authors' knowledge, the first computational model harboring a predictive score of 97.92% and made of nine molecular signatures that discriminate and classify the honey bees’ systemic response to the bacteria is built. Hence, the model is challenged by classifying a training set of hemolymphs and an overall recognition of 91.93% is obtained. Through this work, a novel, time and cost saving high‐throughput strategy that addresses honey bee health on an individual scale is introduced.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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MALDI–MS Profiling to Address Honey Bee Health Status under Bacterial Challenge through Computational Modeling

et al. 2019

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“…These methods showed high diagnostic accuracy in identifying fertile and subfertile males in a large population of known fertility from two distinct fertile and subfertile lineages. The diverse group of proteins thus identified have provided new insights into sperm function (Soler et al, ).…”

Section: Diagnostic Methods To Identify Immune Regulatory Moleculesmentioning

confidence: 99%

Immune regulatory molecules as modifiers of semen and fertility: A review

Archana

Selvaraju

Binsila

et al. 2019

Molecular Reproduction Devel

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Declining fertility rates in both human and animals is a cause for concern. While many of the infertility cases are due to known causes, idiopathic infertility is reported in 30% of the infertile couples. In such cases, 18% of the infertile males carry antisperm antibodies (ASAs). Such data are lacking in livestock, wherein 20–30% of the animals are being culled due to low fertility. In males, the blood–testis barrier (BTB) and biomolecules in the semen provide an immuno‐tolerant microenvironment for spermatozoa as they traverse the immunologic milieu of both the male and female reproductive tracts. For example, insults from environmental contaminants, infections and inflammatory conditions are likely to impact the immune privilege state of the testis and fertility. The female mucosal immune system can recognize allogenic spermatozoa‐specific proteins affecting sperm kinematics and sperm‐zona binding leading to immune infertility. Elucidating the functions and pathways of the immune regulatory molecules associated with fertilization are prerequisites for understanding their impact on fertility. An insight into biomolecules associated with spermatozoal immune tolerance may generate inputs to develop diagnostic tools and modulate fertility. High‐throughput sequencing technologies coupled with bioinformatics analyses provides a path forward to define the array of molecules influencing pregnancy outcome. This review discusses the seminal immune regulatory molecules from their origin in the testis until they traverse the uterine environment enabling fertilization and embryonic development. Well‐designed experiments and the identification of biomarkers may provide a pathway to understand the finer details of reproductive immunology that will afford personalized therapies.

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“…The authors also identified several proteins involved in different biochemical pathways (oxidoreduction mechanisms, energy processes, proteolysis, and protein localization) with a relevant role in rooster fertility. The same group recently conducted a complimentary study [51] in which a larger standardized experimental procedure was designed for the development of a fertility-predictive mathematical model based on sperm cell MALDI-TOF MS profiles acquired through a fast, automated method. The authors determined that an intact cell MALDI-TOF MS-based method showed high diagnostic accuracy in identifying fertile/subfertile males in a large male population of known fertility from two distinct genetic lineages (meat production and egg laying).…”

Section: Top-down Proteomics In Animal Sciencesmentioning

confidence: 99%

Top-Down Proteomics and Farm Animal and Aquatic Sciences

Campos

Almeida

2016

Proteomes

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Proteomics is a field of growing importance in animal and aquatic sciences. Similar to other proteomic approaches, top-down proteomics is slowly making its way within the vast array of proteomic approaches that researchers have access to. This opinion and mini-review article is dedicated to top-down proteomics and how its use can be of importance to animal and aquatic sciences. Herein, we include an overview of the principles of top-down proteomics and how it differs regarding other more commonly used proteomic methods, especially bottom-up proteomics. In addition, we provide relevant sections on how the approach was or can be used as a research tool and conclude with our opinions of future use in animal and aquatic sciences.

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Intact Cell MALDI-TOF MS on Sperm: A Molecular Test For Male Fertility Diagnosis

Cited by 29 publications

References 53 publications

MALDI–MS Profiling to Address Honey Bee Health Status under Bacterial Challenge through Computational Modeling

MALDI–MS Profiling to Address Honey Bee Health Status under Bacterial Challenge through Computational Modeling

Immune regulatory molecules as modifiers of semen and fertility: A review

Top-Down Proteomics and Farm Animal and Aquatic Sciences

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