Glutathione S-transferase in marine invertebrates from Langesundfjord

Lee, RF

doi:10.3354/meps046033

Cited by 45 publications

(21 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This is consistent with other studies of Carcinus maenas in the mesocosrn basins as no difference in either respiration rate or glutathione transferase activity were observed (Bayne & Thurberg 1988, Lee 1988. In comparison with field populations of C. rnaenas, there is no evidence of metabolic turnover of aromatic hydrocarbons or evidence of molting among any of the crab populations from the mesocosm experiment, possibly as a result of the lower temperature in the mesocosm basins.…”

Section: Discussionsupporting

confidence: 91%

“…These responses suggest that alterations in the mobilisation of tnacylglycerols, sterol turnover and tissue degeneration may be taking place. Responses to the contaminant gradient are consistent with observations of changes in respiration rate of C. maenas reported by Bayne & Thurberg (1988) and elevations in glutathione transferase activity for C. maenas reported by Lee (1988); populations at Site 3 appeared to be the most impacted. The responses are not consistent with body burden data of aromatic hydrocarbons nor with the trends observed in PCB concentrations that indicate the highest concentration of PCBs at Site 4.…”

Section: Discussionsupporting

confidence: 85%

See 1 more Smart Citation

Lipid composition of the digestive glands of Mytilus edulis and Carcinus maenas in response to pollutant gradients

Capuzzo¹,

Leavitt²

1988

Mar. Ecol. Prog. Ser.

View full text Add to dashboard Cite

In field stuhes for the GEEP Workshop in Langesundfjord. Norway, changes in lipid content and lipid : protein ratios of digestive glands of Mytilus edulis and Carcinus maenas were reflected along the pollution gradient, with populations of M. edulis showing elevations in both parameters at the 3 most contaminated Sites (2, 3 and 4) in comparison to the reference site (l), and populations of C. maenas showing elevations only at Site 3. In mesocosm experiments only M. edulis from the high dose basin showed elevationsin lipid content and lipid : protein ratios; C. maenasin the medium dose basin showed a decrease in both parameters. Analysis of lipid class composition for field and mesocosm samples of M. edulis reveal differences in response to contaminant gradients that reflect alterations in mobilization of triacylglycerols to phospholipid pools, reductions in phospholipid content, and nutritional condition. Changes in lipid class distributions of M. edulis from both field and mesocosm experiments correlate well with body burden data for tissue concentrations of aromatic hydrocarbons and/or polychlorinated biphenyls. Lipid class dstributions of field samples of C. maenasindicate alterations in the mobilization of triacylglycerols, sterol turnover, and reductions in phospholipid content. The responses, which suggest that crabs from Site 3 are the most impacted, are not consistent with contaminant data from the field sites. Crabs from mesocosm expenments show no evidence of alterations in lipid class distribution in spite of a consistent trend in aromatic hydrocarbon tissue concentrations along the gradient. Differential responses of field and mesocosm populations of M edulis and C. rnaenas are possibly the result of metabolic capacity for detoxification and differences in trophic transfer.

show abstract

Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 85%

Lipid composition of the digestive glands of Mytilus edulis and Carcinus maenas in response to pollutant gradients

Capuzzo¹,

Leavitt²

1988

Mar. Ecol. Prog. Ser.

View full text Add to dashboard Cite

show abstract

“…Interestingly, the heavy metals were found to be highly accumulated in Diplonychus urinator as well as the high enzymes activity, thus both enzymes could be used as a biomarker of water and sediment contamination (Lee, 1988).…”

Section: The Activity Of Detoxification Enzymementioning

confidence: 99%

Valuation of water pollution using enzymatic biomarkers in aquatic insects as bioindicators from El-Mansouriya stream, Dakahlia, Egypt

Bream¹,

Amer²,

Haggag³

et al. 2017

Int. J. Adv. Res. Biol. Sci

View full text Add to dashboard Cite

This investigation was aimed to evaluate water pollution with heavy metals using three biomarkers, as well as the study of the aquatic insects-seasons interaction. Three species of aquatic insects collected from El-Mansouriya stream (Dakahlia, Egypt), during the different seasons from spring 2014 to winter 2015. Water samples, sediments and whole body of the three aquatic insects were analyzed for heavy metal contents. As well as, two enzyme biomarkers, Acetylcholinesterase (AChE) and glutathione-S-transferase (GST) were used to monitor aquatic insects' response to water pollutants. Results showed various activities of the three biomarkers at the different studied seasons. Where, the concentration of selected heavy metals, in the whole body of the three insects, showed a relatively wide range for each metal analyzed. Also, higher values of metal pollution index (MPI) were attained in hotter seasons (spring and summer) and its decrements were observable during cooler seasons (autumn, winter), that provides a representative picture to the environmental state of environmental impacts on the aquatic ecosystem. While the heavy metals were found to be highly accumulated in selected aquatic insects body, as well as the variation of enzymes activity, thus both enzymes could be used as biomarker of water and sediment contamination.

show abstract

“…The majority of studies reporting GST activity from molluscs have used GST activity measurements as an indicator of pollutant exposure [13,[42][43][44][45]. Although the level of GST activity in C. gibbosum is high even in comparison to that of molluscs exposed to pollutants known to induce GST expression [46][47][48], this high activity is unlikely to be a response to anthropogenic contamination, given the remote site of collection. Rather, the high GST activity may reflect an adaptation that facilitates consumption of allelochemical-rich prey.…”

Section: Discussionmentioning

confidence: 99%

Proteomic identification, cDNA cloning and enzymatic activity of glutathione S-transferases from the generalist marine gastropod, Cyphoma gibbosum

Whalen¹,

Morin²,

Lin³

et al. 2008

Archives of Biochemistry and Biophysics

View full text Add to dashboard Cite

Short title: Characterization of glutathione S-transferases from Cyphoma gibbosumFootnote: The nucleotide and translated amino acid sequences for C. gibbosum GSTs have been deposited in GenBank with the following accession nos. EU008563 (CgGSTM1) and EU008562 (CgGSTM2).Abbreviations footnote: CDNB, 1-chloro-2,4-dinitrobenzene; GST, glutathione S-transferase; nrDB, non-redundant database; RACE, rapid amplification of cDNA ends; RT-PCR, reverse transcriptase polymerase chain reaction 2 ABSTRACT Glutathione S-transferases (GST) were characterized from the digestive gland of Cyphoma gibbosum (Mollusca; Gastropoda), to investigate the possible role of these detoxification enzymes in conferring resistance to allelochemicals present in its gorgonian coral diet. We identified the collection of expressed cytosolic Cyphoma GST classes using a proteomic approach involving affinity chromatography, HPLC and nanospray liquid chromatography-tandem mass spectrometry (LC-MS/MS). Two major GST subunits were identified as putative mu-class GSTs; while one minor GST subunit was identified as a putative theta-class GST, apparently the first theta-class GST identified from a mollusc. Two Cyphoma GST cDNAs (CgGSTM1 and CgGSTM2) were isolated by RT-PCR using primers derived from peptide sequences. Phylogenetic analyses established both cDNAs as mu-class GSTs and revealed a mollusc-specific subclass of the GST-mu clade. These results provide new insights into metazoan GST diversity and the biochemical mechanisms used by marine organisms to cope with their chemically defended prey.

show abstract

Glutathione S-transferase in marine invertebrates from Langesundfjord

Cited by 45 publications

References 17 publications

Lipid composition of the digestive glands of Mytilus edulis and Carcinus maenas in response to pollutant gradients

Lipid composition of the digestive glands of Mytilus edulis and Carcinus maenas in response to pollutant gradients

Valuation of water pollution using enzymatic biomarkers in aquatic insects as bioindicators from El-Mansouriya stream, Dakahlia, Egypt

Proteomic identification, cDNA cloning and enzymatic activity of glutathione S-transferases from the generalist marine gastropod, Cyphoma gibbosum

Contact Info

Product

Resources

About