Glutathione S-transferase (GST) family in barley: Identification of members, enzyme activity, and gene expression pattern

Rezaei, Mohammad; Shobbar, Zahra‐Sadat; Shahbazi, Maryam; Abedini, Raha; Zare, Samira

doi:10.1016/j.jplph.2013.04.005

Cited by 109 publications

(79 citation statements)

References 61 publications

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“…55 GSTs in Arabidopsis thaliana (Dixon et al 2009), 82 in Oryza sativa , 81 in Populus (Lan et al 2009), 42 in maize (McGonigle et al 2000), 65 in broccoli (Vijayakumar et al 2016), 84 in Hordeum vulgare (Rezaei et al 2013), 20 in Draceana cambodiana (Zhu et al 2016), and 37 GSTs in Physcomitrella patens (a bryophyte) have been identified (Liu et al 2013). …”

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confidence: 99%

In silico genome-wide identification and characterization of the glutathione S-transferase gene family in Vigna radiata

Vaish

Awasthi

Tiwari

et al. 2018

Genome

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Plant glutathione S-transferases are integral to normal plant metabolism, and biotic and abiotic stress tolerance. GST gene family has been characterized in diverse plant species using molecular biology and bioinformatics approaches. In the current study, in silico analysis identified 44 GSTs in Vigna radiata. Of the total 44 GSTs identified, chromosomal locations of 31 GSTs were confirmed. The pI value of GST proteins ranged from 5.10 to 9.40. The predicted molecular weights ranged from 13.12 to 50 kDa. Subcellular localization analysis revealed that all GSTs were predominantly localized in the cytoplasm. The active site amino acids were confirmed to be serine in tau, phi, theta, zeta and TCHQD; cysteine in lambda, DHAR and omega; and tyrosine in EF1G. The gene architecture conformed to the 2 exon-1 intron and 3 exon-2 intron organization in case of tau and phi classes, respectively. MEME analysis identified 10 significantly conserved motifs with the width of 8 to 50 amino acids. The motifs identified were either specific to a specific GST class, or were shared by multiple GST classes. The results of the current study will be of potential importance in the characterization of GST gene family in V. radiata, an economically important leguminous crop.

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confidence: 99%

In silico genome-wide identification and characterization of the glutathione S-transferase gene family in Vigna radiata

Vaish

Awasthi

Tiwari

et al. 2018

Genome

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“…1). Onion GSTs were previously reported in Japanese onion by Rohman et al [11,38] where five GST peaks eluted from ion exchange chromatography and among them a Phi type GST was reported with its function regarding legand binding and salinity drought and cold tolerance. On the other hand, another Allium crop garlic has been used to study GST in animal health, however, role of garlic GST has not been studied the biological role of GST in garlic plants under normal and abiotic stresses.…”

Section: Comparative Investigation Of Gst Activities In Different Cropsmentioning

confidence: 99%

Comparative Investigation of Glutathione S-transferase (GST) in Different Crops and Purification of High Active GSTs from Onion (Allium cepa L.)

Islam¹

2015

JPS

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Glutathione S-transferases (GSTs; EC 2.5.1.18) are abundant proteins encoded by a highly divergent ancient gene family with protective functions through detoxification and non-catalytic roles as carriers of cytotoxins. In this work, we investigated the GST activities in seedlings of 38 crops to obtain a plant with high active GST for further study. In screening of 38 crops, onion seedling showed the highest GST activity (483.54 nmol min -1 mg -1 protein) followed by wheat(372.89 nmol min -1 mg -1 protein), barley (253.44 nmol min -1 mg -1 protein), rice (244.12 nmol min -1 mg -1 protein) and proso millet (173.34 nmol min -1 mg -1 protein). Carrot seedling showed the lowest activity (3.63 nmol min -1 mg -1 protein).In onion plants, both root and leaf showed high GST activity. Onion bulb GSTs were separated DEAE cellulose column chromatography, and purified by affinity chromatography (S-hexyl glutathione-agarose). Three GST peaks were found to elute at 67, 107 and 140mM of KCl gradient solution, and were named as GSTa, GSTb and GSTc. Among the three GSTs, GSTa, GSTb and GSTc contained 9.58, 61.45 and 28.97% of total activity, respectively. In purification, GSTa, GSTb and GSTc had specific activities of 9075, 17259 and 19868 nmol min -1 mg -1 protein, respectively, along with yield of 2.48, 3.17 and 1.28,and purification fold of 15.8, 29.9 and 34.5, respectively. The purity and molecular mass of the fraction was examined by SDS-PAGE. The silver staining of the purified GSTa, GSTb and GSTc indicated that final product of GSTb and GSTc were highly purified and migrated as a single band on SDS-PAGE with an apparent molecular mass of 27 kDa. However, GSTa eluted with Glyoxalase-I (Gly-I) and to purify GSTa, more methodological application are suggested.

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“…However, after treatment with elemene, unmethylated GSTP1 genes were found in the QGY7703 cells. The GSTP1 gene is located at q13 of human chromosome 11 and encoded an enzyme with detoxicating and protein-binding effects (37). The GSTP1 protein's main function is to catalyze the reactions between glutathione and electron-containing compounds, which could help metabolize carcinogens and exogenous drugs into lowor non-toxic metabolites and thus exert anti-cytotoxic and anti-carcinogen effects (37).…”

Section: Different Concentrations Of Elemene (µG/ml) ----------------mentioning

confidence: 99%

“…The GSTP1 gene is located at q13 of human chromosome 11 and encoded an enzyme with detoxicating and protein-binding effects (37). The GSTP1 protein's main function is to catalyze the reactions between glutathione and electron-containing compounds, which could help metabolize carcinogens and exogenous drugs into lowor non-toxic metabolites and thus exert anti-cytotoxic and anti-carcinogen effects (37). Several previous studies showed that GSTP1 inactivation induced by hypermethylation is mainly found in several human tumors, including prostate, renal, breast, and liver cancers (10-12).…”

Section: Different Concentrations Of Elemene (µG/ml) ----------------mentioning

confidence: 99%

Demethylation effects of elemene on the GSTP1 gene in HCC cell line QGY7703

Jiang

Zhu

et al. 2016

Oncology Letters

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Abstract. The present study aimed to investigate elemene's effects on cell proliferation, apoptosis, and the cell cycle in the hepatocellular carcinoma (HCC) cell line, QYG7703, and to investigate GSTP1 gene methylation change in QGY7703 cells after being treated with elemene to explore whether elemene reversed the abnormal GSTP1 gene methylation. QGY7703 cells were treated with different elemene concentrations. Cell proliferation was measured with MTT assay, cell apoptosis and cell cycle were analyzed by flow cytometry, and GSTP1 gene methylation was analyzed by methlation-specific polymerase chain reaction. The cells' apoptotic rate increased dose-dependently with elemene concentration, and the difference was statistically significant (P<0.05). Elemene treatment arrested the cells in S phase, and thus the percentage of cells in G1 phase decreased while the cells in S phase increased dose-dependently, and the difference was statistically significant compared to the control group (P<0.05). All QGY7703 cells were identified to contain GSTP1 gene methylation before being treated with elemene and the methylation state decreased after treatment. In the present study, elemene induced cell apoptosis, inhibited the cell cycle, and reversed GSTP1 gene methylation in QGY7703 cells.

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Glutathione S-transferase (GST) family in barley: Identification of members, enzyme activity, and gene expression pattern

Cited by 109 publications

References 61 publications

In silico genome-wide identification and characterization of the glutathione S-transferase gene family in Vigna radiata

In silico genome-wide identification and characterization of the glutathione S-transferase gene family in Vigna radiata

Comparative Investigation of Glutathione S-transferase (GST) in Different Crops and Purification of High Active GSTs from Onion (Allium cepa L.)

Demethylation effects of elemene on the GSTP1 gene in HCC cell line QGY7703

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