Glutathione S-Transferase activities and glutathione levels in needles of drought stressed Pinus brutia Ten. trees

Yılmaz, Can; İşcan, Mümtaz

doi:10.5505/tjb.2014.88319

Cited by 12 publications

(5 citation statements)

References 25 publications

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“…Glutathione S-transferases are involved in protection under various stress conditions by detoxifying endogenous plant toxins which increases under oxidative stress [ 79 ]. GSTs play a major role under drought conditions by conjugation of glutathione with electrophilic substrates to increase their solubility and facilitating further metabolic processing [ 80 ]. There are reports, which suggest involvement of GSTs in drought stress [ 81 , 82 ].…”

Section: Discussionmentioning

confidence: 99%

Transcriptome analysis of lentil (Lens culinaris Medikus) in response to seedling drought stress

et al. 2017

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BackgroundDrought stress is one of the most harmful abiotic stresses in crop plants. As a moderately drought tolerant crop, lentil is a major crop in rainfed areas and a suitable candidate for drought stress tolerance research work. Screening for drought tolerance stress under hydroponic conditions at seedling stage with air exposure is an efficient technique to select genotypes with contrasting traits. Transcriptome analysis provides valuable resources, especially for lentil, as here the information on complete genome sequence is not available. Hence, the present studies were carried out.ResultsThis study was undertaken to understand the biochemical mechanisms and transcriptome changes involved in imparting adaptation to drought stress at seedling stage in drought-tolerant (PDL-2) and drought-sensitive (JL-3) cultivars. Among different physiological and biochemical parameters, a significant increase was recorded in proline, glycine betaine contents and activities of SOD, APX and GPX in PDL-2 compared to JL-3while chlorophyll, RWC and catalase activity decreased significantly in JL-3. Transcriptome changes between the PDL-2 and JL-3 under drought stress were evaluated using Illumina HiSeq 2500 platform. Total number of bases ranged from 5.1 to 6.7 Gb. Sequence analysis of control and drought treated cDNA libraries of PDL-2 and JL-3 produced 74032, 75500, 78328 and 81523 contigs, respectively with respective N50 value of 2011, 2008, 2000 and 1991. Differential gene expression of drought treated genotypes along with their controls revealed a total of 11,435 upregulated and 6,934 downregulated transcripts. For functional classification of DEGs, KEGG pathway annotation analysis extracted a total of 413 GO annotation terms where 176 were within molecular process, 128 in cellular and 109 in biological process groups.ConclusionThe transcriptional profiles provide a foundation for deciphering the underlying mechanism for drought tolerance in lentil. Transcriptional regulation, signal transduction and secondary metabolism in two genotypes revealed significant differences at seedling stage under severe drought. Our finding suggests role of candidate genes for improving drought tolerance in lentil.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-017-3596-7) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Transcriptome analysis of lentil (Lens culinaris Medikus) in response to seedling drought stress

et al. 2017

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“…Determination of glutathione S-transferase (GST) activity was performed in line with the method given by Habig et al [30]. Optimization was performed for this using the ELISA Microplate Reader System, which was used on a Multiskan FC microplate photometer (Thermo Fisher Scienti c Inc., Waltham, MA, USA) (Yilmaz and Işcan, 2014) [31]. The contents of the reaction medium for the total GST activity measurements were 0.1 M of phosphate buffer at a pH of 7.4, 1 mM of 1-chloro-2,4-dinitrobenzene, and 1 mM of GSH.…”

Section: Measurement Of Antioxidant Defense Indicatorsmentioning

confidence: 99%

Bisphenol S leads to cytotoxicity-induced antioxidant responses and oxidative stress in isolated rainbow trout (Oncorhyncus mykiss) hepatocytes

et al. 2021

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Background: Bisphenol S (BPS) is a chemical compound that is utilized in the plastic industry as an alternative to bisphenol A (BPA). The toxic effects of BPS in sh is less known and limited. Therefore, in the present study, it was aimed to investigate the in uence of BPS on rainbow trout (Oncorhyncus mykiss) hepatocytes in vitro.Methods and Results: For this purpose, the hepatocytes of the sh were isolated, and then the cultured cells were treated with increasing concentrations of BPS (0, 15.63, 31.25, 62.50, 125, 250, and 500 µM) for 24 h. The cytotoxic impact of BPS was determined in the culture media using lactate dehydrogenase assay and then, the antioxidant defence indicators were assayed. The results showed that concentrationdependent increases were observed in the percentage of cytotoxicity. The superoxide dismutase activity was reduced, while the catalase and glutathione peroxidase activity was elevated with all of the BPS concentrations. The glutathione S-transferase (GST) activity was signi cantly increased with a BPS concentration of 31.25 µM or higher, while GST theta 1-1 activity was decreased with the same concentrations of BPS. The reduced glutathione content was decreased signi cantly with a BPS concentration of 31.25 µM or higher, and the malondialdehyde content increased with BPS concentrations of 125, 250, and 500 µM.Conclusions: The ndings determined herein suggested that BPS causes cytotoxicity in sh hepatocytes and could lead to oxidative stress, resulting hepatotoxicity in sh. Thus, the utilization of BPS instead of BPA as safe alternative in industry should be re-evaluated in the future for environmental health.

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“…Glutathione transferases are involved in protection under various stress conditions by detoxifying toxins, increasing oxidative stress (Singh et al 2017). Glutathione S-transferases play a signi cant role under drought conditions by conjugating glutathione with electrophilic substrates to increase their solubility and facilitate further metabolic processing (Yilmaz and Iscan 2014). Expression levels of glutathione S-transferase and glutathione peroxidase activity under drought in ag leaves were found correlated with high yield stability (Galle et al 2009).…”

Section: Degs Involved In Metabolites Pathways and Their Expression Pmentioning

confidence: 99%

Thirty years of genetic progress combined with seed ethylene priming improved drought resistance of wheat through long-term drought stress memory

Yang

Zhao

et al. 2021

Preprint

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Knowledge of short-term physiological adaption of wheat to soil water deficit is well understood, but little is known about seed ethylene priming effect on long-term drought stress memory of dryland winter wheat. The 42 leading and new cultivars released between 1992 to 2017 were subjected to drought (45% Field water-holding capacity), and well-watered (75% Field water-holding capacity) conditions aim to screen cultivars with contrasting drought resistance and grain yield. Seeds primed with ethylene were subjected to both PEG-8000 and pure water to uncover ethylene-induced stress memory at both physiological and organ levels. Results showed that the soil water deficit (45%FC) that occurred at the tillering stage resulted in 3.2% to 67.4% yield loss for 42 cultivars, which was determined mainly by the decrease in the fertile spike. Seed ethylene priming maintained leaf water by reducing root volume and dry weight, which played a crucial role in drought avoidance. Seed ethylene priming decreased malondialdehyde content by regulating auxin and abscisic acid signaling, reactive oxygen species scavenging capability, and osmotic regulation, which plays a crucial role in drought tolerance. Seed ethylene priming improved drought tolerance of the wheat through metabolic modification of carbon metabolism, glutathione metabolism, and phenylpropanoid biosynthesis . The seed ethylene priming induced long-term stress memory that improved tillering capacity and reduced wheat spikelets abortion, which provided extra 0.3 t ha -1 of grain yield. These results suggested that seed ethylene priming allowed the recall of long-lasting stress defensive memory, increasing grain yield by both drought avoidance and drought tolerance.

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Glutathione S-Transferase activities and glutathione levels in needles of drought stressed Pinus brutia Ten. trees

Cited by 12 publications

References 25 publications

Transcriptome analysis of lentil (Lens culinaris Medikus) in response to seedling drought stress

Transcriptome analysis of lentil (Lens culinaris Medikus) in response to seedling drought stress

Bisphenol S leads to cytotoxicity-induced antioxidant responses and oxidative stress in isolated rainbow trout (Oncorhyncus mykiss) hepatocytes

Thirty years of genetic progress combined with seed ethylene priming improved drought resistance of wheat through long-term drought stress memory

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