Glucuronoxylan-based quince seed hydrogel: A promising scaffold for tissue engineering applications

Güzelgülgen, Meltem; Ozkendir‐Inanc, Dilce; Yıldız, Ümit Hakan; Arslan‐Yildiz, Ahu

doi:10.1016/j.ijbiomac.2021.03.096

Cited by 31 publications

(23 citation statements)

References 59 publications

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“…The high xylose content in the mixture of the aspen HC monosaccharides indicates that glucuronoxylan is the leading polysaccharide in the obtained HCs, which is typical of hardwood HCs [ 36 , 37 ]. The presence of mannose, galactose, and glucose is indicative of glucomannan, arabinogalactan, and other polysaccharides in small amounts [ 38 ].…”

Section: Resultsmentioning

confidence: 99%

Composition and Structure of Aspen (Pópulus trémula) Hemicelluloses Obtained by Oxidative Delignification

et al. 2022

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In this study, hemicelluloses of aspen wood (Pópulus trémula) were obtained by oxidative delignification in an acetic acid‒water‒hydrogen peroxide medium at temperatures of 70‒100 °C and a process time of 1‒4 h. The maximum polysaccharide yield of up to 9.68 wt% was reported. The composition and structure of the hemicelluloses were studied using a complex of physicochemical methods: gas and gel permeation chromatography, Fourier-transform infrared spectroscopy, 2D nuclear magnetic resonance spectroscopy, and thermogravimetric analysis. The xylose, mannose, galactose, and glucose monomer units were identified in the hemicelluloses by gas chromatography. The weight average molecular weight Mw of the products determined by gel permeation chromatography was found to range within 8932‒33,142 g/mol. The reported Fourier-transform spectra of the hemicelluloses contain all the bands characteristic of heteropolysaccharides; a weak lignin absorption signal in the spectra at 1500‒1510 cm−1 is attributed to a minor content of phenolic fragments in the structure of the obtained hemicelluloses. The use of thermogravimetric analysis established that the hemicelluloses isolated from aspen wood are resistant against heating to temperatures of up to 90–100 °C and, upon further heating up to 400 °C, start destructing at an increasing rate. The antioxidant activity of the hemicelluloses was examined using the compounds that mimic free radicals (1,1-diphenyl-2-picrylhydrazyl) and hydroxyl radicals (salicylic acid). It was found that the activity of all polysaccharides in neutralizing DPPH and hydroxyl radicals is lower than the absorption capacity of vitamin C at all the tested concentrations (0.5, 2, and 5 mg/mL) and attains 81.7 and 82.9%, respectively.

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Section: Resultsmentioning

confidence: 99%

Composition and Structure of Aspen (Pópulus trémula) Hemicelluloses Obtained by Oxidative Delignification

et al. 2022

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“…The images at every unit degree change in temperature were recorded during the analysis. The raw data was then analyzed in graphical form using Origin Pro ® 2021 software (OriginLab Corporation, Northampton, MA, USA) [51].…”

Section: Thermal Analysismentioning

confidence: 99%

Polymeric Microparticles: Synthesis, Characterization and In Vitro Evaluation for Pulmonary Delivery of Rifampicin

et al. 2022

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Rifampicin, a potent broad-spectrum antibiotic, remains the backbone of anti-tubercular therapy. However, it can cause severe hepatotoxicity when given orally. To overcome the limitations of the current oral therapy, this study designed inhalable spray-dried, rifampicin-loaded microparticles using aloe vera powder as an immune modulator, with varying concentrations of alginate and L-leucine. The microparticles were assessed for their physicochemical properties, in vitro drug release and aerodynamic behavior. The spray-dried powders were 2 to 4 µm in size with a percentage yield of 45 to 65%. The particles were nearly spherical with the tendency of agglomeration as depicted from Carr’s index (37 to 65) and Hausner’s ratios (>1.50). The drug content ranged from 0.24 to 0.39 mg/mg, with an association efficiency of 39.28 to 96.15%. The dissolution data depicts that the in vitro release of rifampicin from microparticles was significantly retarded with a higher L-leucine concentration in comparison to those formulations containing a higher sodium alginate concentration due to its hydrophobic nature. The aerodynamic data depicts that 60 to 70% of the aerosol mass was emitted from an inhaler with MMAD values of 1.44 to 1.60 µm and FPF of 43.22 to 55.70%. The higher FPF values with retarded in vitro release could allow sufficient time for the phagocytosis of synthesized microparticles by alveolar macrophages, thereby leading to the eradication of M. tuberculosis from these cells.

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“…The result was consistent with the morphology observation. ADM sponge further crosslinked by the glutaraldehyde formed a firmer spongy structure connected by chemical covalent bonding (Guzelgulgen et al, 2021;Piola et al, 2022). Therefore, the pore interconnection inside the structure and total porosity decreased.…”

Section: Analysis Of Physicochemical Propertiesmentioning

confidence: 99%

A Comparative Study on Two Types of Porcine Acellular Dermal Matrix Sponges Prepared by Thermal Crosslinking and Thermal-Glutaraldehyde Crosslinking Matrix Microparticles

Huang

Ding

Pan³

et al. 2022

Front. Bioeng. Biotechnol.

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Common commercial porcine acellular dermal matrix (PADM) products take the form of a thin membrane. Given its dense structure, delaying vascularization after implantation remains an issue to be solved. In addition, overlaying multiple sheets to address deep wounds and large tissue defects that are difficult to repair by self-tissues could hinder tissue ingrowth, angiogenesis, and integration. Here, we creatively prepared PADM microparticles through a homogenizing treatment and crosslinked them to ADM sponges by thermal crosslinking (VT-ADM) and thermal-glutaraldehyde crosslinking (GA-ADM). The resulting VT-ADM was thicker than GA-ADM, and both maintained the natural dermal matrix microstructure and thermal stability. The porosity of GA-ADM (mean 82%) was lower than that of VT-ADM (mean 90.2%), but the mechanical strength and hydrophilicity were significantly higher. The two types of ADM sponges showed no obvious difference in cell adhesion and proliferation without cytotoxicity. Furthermore, the human adipose stem cells were co-cultured with ADM sponges which promoted proliferation, tube formation, and migration of endothelial cells, and the GA-ADM group exhibited better migration behavior. There were no markable differences among expressions of pro-angiogenesis genes, including vascular endothelial growth factor, insulin-like growth factor-1, and epidermal growth factor. In a nude mouse model, the VT-ADM and GA-ADM pre-cultured with human adipose stem cells for 1 week in advance were implanted subcutaneously. The VT-ADM and the GA-ADM showed great histocompatibility without local redness, swelling, or necrosis. The vascular density of the local skin flap above the material was visualized using indocyanine green and showed no statistical difference between the two groups. The collagen tissue deposition in the pores and vessel formation within the sponges increased with time. Although VT-ADM had a higher degradation rate in vivo, the integrity of the two scaffolds was preserved. Collectively, the VT-ADM and the GA-ADM retained a natural matrix structure and presented biocompatibility. Thus, the above-mentioned two crosslinking methods for ADM sponges are safe and practicable. The novel ADM sponges with good physicochemical and biological properties are no longer limited to membrane tissue regeneration but could also realize structure remodeling where they act as scaffolds for a soft tissue filler and three-dimensional reconstruction of the tissue with strength requirements.

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Glucuronoxylan-based quince seed hydrogel: A promising scaffold for tissue engineering applications

Cited by 31 publications

References 59 publications

Composition and Structure of Aspen (Pópulus trémula) Hemicelluloses Obtained by Oxidative Delignification

Composition and Structure of Aspen (Pópulus trémula) Hemicelluloses Obtained by Oxidative Delignification

Polymeric Microparticles: Synthesis, Characterization and In Vitro Evaluation for Pulmonary Delivery of Rifampicin

A Comparative Study on Two Types of Porcine Acellular Dermal Matrix Sponges Prepared by Thermal Crosslinking and Thermal-Glutaraldehyde Crosslinking Matrix Microparticles

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