Glucose uptake kinetics of Saccharomyces cerevisiae monitored with a newly developed FIA

Rothen, S. A.; Saner, M.; Meenakshisundaram, S.; Sonnleitner, Bernhard; Fiechter, Armin

doi:10.1016/0168-1656(96)01500-3

Cited by 13 publications

(7 citation statements)

References 21 publications

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“…The glucose profile showed a characteristic tail from 1-3 h as the glucose concentration decreased from 180 to 100 mg/l. A similar glucose response has also been observed by Rothen et al (1996).…”

Section: Dilution Rate Shift-up/down Experimentssupporting

confidence: 80%

Dynamic effects related to steady‐state multiplicity in continuous Saccharomyces cerevisiae cultivations

Lei

Olsson

Jørgensen

2004

Biotech & Bioengineering

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The behavioral differences between chemostat and productostat cultivation of aerobic glucose-limited Saccharomyces cerevisiae were investigated. Three types of experiments were conducted: a chemostat, where the dilution rate was shifted up or down in stepwise manner; and a productostat, with either stepwise changed or a rampwise increased ethanol setpoint, i.e., an accelero-productostat. The transient responses from chemostat and productostat experiments were interpreted using a simple metabolic flux model. In a productostat it was possible to obtain oxido-reductive steady states at dilution rates far below Dcrit due to a strong repression of the respiratory system. However, these steady states could not be obtained in a chemostat, since a dilution rate shift-down from an oxido-reductive steady state led to a derepression of the respiratory system. It can therefore be concluded that the range of dilution rates where steady-state multiplicity can be obtained differs depending on the operation mode and that this dilution rate multiplicity range may appear larger in a productostat than in a chemostat. A more narrow multiplicity range, however, was obtained when the productostat was operated as an accelero-productostat.

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Section: Dilution Rate Shift-up/down Experimentssupporting

confidence: 80%

Dynamic effects related to steady‐state multiplicity in continuous Saccharomyces cerevisiae cultivations

Lei

Olsson

Jørgensen

2004

Biotech & Bioengineering

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“…Benthin et al [13] used numerical values for the time constant of mutarotation (kmut) in the order of 2.8 to ll.4h -1. We have determined kmut-value = 1.2 ___ 0.02 h-1 in pure water and 12 + 1 h-1 in phosphate buffer as well as in defined E. coli growth medium independent of whether starting from pure ~-or fl-form [14]. These numerical values of the time constants confirm that there is no time for significant mutarotation to take place in rapid on-line analyses.…”

Section: Systematic Analytical Errorssupporting

confidence: 53%

New concepts for quantitative bioprocess research and development

Sonnleitner

1996

Advances in Biochemical Engineering/Biotechnology

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“…It should right from the start be emphasized that the time constants considered are in the range of several hours. Consequently the model will never explain the rapid control mechanisms studied by van Dam and co-workers (de Koning and van Dam, 1992;Walsh et al, 1994), Reuss and co-workers (Rizzi et al, 1996;Theobald et al, 1997), and Sonnleitner and co-workers (Rothen et al, 1996;Sonnleitner et al, 1997).…”

Section: Introductionmentioning

confidence: 94%

Simple generic model for dynamic experiments withSaccharomyces cerevisiae in continuous culture: Decoupling between anabolism and catabolism

Duboc

Stockar

1998

Biotechnol. Bioeng.

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The dynamic behavior of a continuous culture of Saccharomyces cerevisiae subjected to a sudden increase in the dilution rate has been successfully modelled for anaerobic growth on glucose, and for aerobic growth on acetate, on ethanol, and on glucose. The catabolism responded by an immediate jump whereas biosynthesis did not. Thus catabolism was in excess to anabolism. The model considers the decoupling between biosynthesis and catabolism, both types of reactions being modelled by first‐order kinetic expressions evolving towards maximal values. Yield parameters and maximal reaction rates were identified in steady state continuous cultures or during batch experiments. Only the time constant of biosynthesis regeneration, τX, and the time constant of catabolic capacity regeneration, τcat, had to be identified during transient experiments. In most experiments τX was around 3 h, and τcat varied between 2 and 2.5 h for the different metabolisms investigated. © 1998 John Wiley & Sons, Inc. Biotechnol Bioeng 60: 180–189, 1998.

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Glucose uptake kinetics of Saccharomyces cerevisiae monitored with a newly developed FIA

Cited by 13 publications

References 21 publications

Dynamic effects related to steady‐state multiplicity in continuous Saccharomyces cerevisiae cultivations

Dynamic effects related to steady‐state multiplicity in continuous Saccharomyces cerevisiae cultivations

New concepts for quantitative bioprocess research and development

Simple generic model for dynamic experiments withSaccharomyces cerevisiae in continuous culture: Decoupling between anabolism and catabolism

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