Glucosamine promotes seizure activity via activation of the PI3K/Akt pathway in epileptic rats

Yang, Jin; Feng, Ge; Chen, Mingyue; Wang, Shenglin; Tang, Feng Ru; Zhou, Jing; Bao, Nana; Yu, Jiang; Jiang, Guohui

doi:10.1016/j.eplepsyres.2021.106679

Cited by 4 publications

(3 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The rats were anesthetized with intraperitoneal injection of 1% sodium pentobarbital (40 mg/kg) and eye lubrication was used to minimize drying throughout the procedure. One microliter of AAV was stereotactically injected into each of the bilateral hippocampi (0.1 μl/min; coordinates: AP, − 3.6 mm, ML, − 2.8 mm, DV, − 3.5 mm) using a microinjector (Gaoge, Shanghai, China) (10 μl capacity) within 10 min [45]. Rats in the experimental group were infected with the miR-9-5p-carrying virus (n = 11), while those in the vehicle group were injected with an empty adenovirus (n = 11).…”

Section: Experimental Animal Groupingmentioning

confidence: 99%

“…The subcutaneous tissue and periosteum were separated bluntly with cotton swabs to minimize the extent of injury and bleeding in order to fully expose the skull. The position of the right hippocampus (AP − 3.6 mm, ML − 2.8 mm, DV − 3.5 mm) was determined and marked on the skull surface [45]. The skull window was covered with dental cement H-frame for recording hippocampal local field potentials (LFPs) [49,50].…”

Section: Surgical Procedures and Electrophysiological Recordingsmentioning

confidence: 99%

“…The lower end of the microfilament electrode was placed close to the brain tissue using a microdriver [27]. LFPs were pre-amplified (× 1000), filtered (0.1-1000 Hz), and digitized at 4 kHz using an OmniPlex ® D Neural Data Acquisition System (Plexon Inc., Dallas, TX) [45,51]. Baseline LFPs were recorded for 10 min followed by intraperitoneal injection of atropine.…”

Section: Surgical Procedures and Electrophysiological Recordingsmentioning

confidence: 99%

See 2 more Smart Citations

Potentials of miR-9-5p in promoting epileptic seizure and improving survival of glioma patients

Wang

Bao

et al. 2022

Acta Epileptologica

Self Cite

View full text Add to dashboard Cite

Background Epilepsy affects over 70 million people worldwide; however, the underlying mechanisms remain unclear. MicroRNAs (miRNAs) have essential functions in epilepsy. miRNA-9, a brain-specific/enriched miRNA, plays a role in various nervous system diseases and tumors, but whether miRNA-9 is involved in epilepsy and glioma-associated epilepsy remains unknown. Therefore, we aimed to explore the potential role of miR-9-5p in seizures and its effect on the survival of glioma patients, in order to provide new targets for the treatment of epilepsy and glioma. Methods The YM500v2 database was used to validate the expression of hsa-miR-9-5p in tissues. Moreover, qRT-PCR was performed to investigate the expression of miR-9-5p in temporal lobe epilepsy patients and rats with lithium-pilocarpine-induced seizures. Recombinant adeno-associated virus containing miR-9-5p was constructed to overexpress miR-9-5p in vivo. The effects of miR-9-5p on the behavior and electroencephalographic activities of the lithium-pilocarpine rat model of epilepsy were tested. Bioinformatics analysis was used to predict the targets of miR-9-5p and explore its potential role in epilepsy and glioma-associated epilepsy. Results The expression of miR-9-5p increased at 6 h and 7 days after lithium-pilocarpine-induced seizures in rats. Overexpression of miR-9-5p significantly shortened the latency of seizures and increased seizure intensity at 10 min and 20 min after administration of pilocarpine (P < 0.05). Predicted targets of miR-9-5p were abundant and enriched in the brain, and affected various pathways related to epilepsy and tumor. Survival analysis revealed that overexpression of miR-9-5p significantly improved the survival of patients from with low-grade gliomas and glioblastomas. The involvement of miR-9-5p in the glioma-associated epileptic seizures and the improvement of glioma survival may be related to multiple pathways, including the Rho GTPases and hub genes included SH3PXD2B, ARF6, and ANK2. Conclusions miR-9-5p may play a key role in promoting epileptic seizures and improving glioma survival, probably through multiple pathways, including GTPases of the Rho family and hub genes including SH3PXD2B, ARF6 and ANK2. Understanding the roles of miR-9-5p in epilepsy and glioma and the underlying mechanisms may provide a theoretical basis for the diagnosis and treatment of patients with epilepsy and glioma.

show abstract

Section: Experimental Animal Groupingmentioning

confidence: 99%

Section: Surgical Procedures and Electrophysiological Recordingsmentioning

confidence: 99%

Section: Surgical Procedures and Electrophysiological Recordingsmentioning

confidence: 99%

See 1 more Smart Citation

Potentials of miR-9-5p in promoting epileptic seizure and improving survival of glioma patients

Wang

Bao

et al. 2022

Acta Epileptologica

Self Cite

View full text Add to dashboard Cite

show abstract

Research progress on the role of inflammatory mediators in the pathogenesis of epilepsy

Yu,

Sun

2024

Ibrain

View full text Add to dashboard Cite

Epilepsy is an abnormal neurologic disorder distinguished by the recurrent manifestation of seizures, and the precise underlying mechanisms for its development and progression remain uncertain. In recent years, the hypothesis that inflammatory mediators and corresponding pathways contribute to seizures has been supported by experimental results. The potential involvement of neuroinflammation in the development of epilepsy has garnered growing interest. This review centers attention on the involvement of inflammatory mediators in the emergence and progression of epilepsy within recent years, focusing on both clinical research and animal models, to enhance comprehension of the intricate interplay between brain inflammation and epileptogenesis.

show abstract

Arisaema Cum Bile possesses significantly antiepileptic effects through regulation of PI3K/Akt/Nrf2 signaling pathways

Zhong,

He,

et al. 2024

Journal of Taibah University for Science

View full text Add to dashboard Cite

Glucosamine promotes seizure activity via activation of the PI3K/Akt pathway in epileptic rats

Cited by 4 publications

References 40 publications

Potentials of miR-9-5p in promoting epileptic seizure and improving survival of glioma patients

Potentials of miR-9-5p in promoting epileptic seizure and improving survival of glioma patients

Research progress on the role of inflammatory mediators in the pathogenesis of epilepsy

Arisaema Cum Bile possesses significantly antiepileptic effects through regulation of PI3K/Akt/Nrf2 signaling pathways

Contact Info

Product

Resources

About