Glucoamylase production by solid-state fermentation using rice flake manufacturing waste products as substrate

Anto, Hema; Trivedi, Ujjval; Patel, K. C.

doi:10.1016/j.biortech.2005.05.007

Cited by 115 publications

(79 citation statements)

References 12 publications

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“…Glucoamylase activity was determined in a reaction mixture containing 1 ml of enzyme solution appropriately diluted in McIlvaine's buffer with 1 ml of aqueous suspension of 1% starch at 50°C for 10 min (Anto et al, 2006). In these enzymes test the reaction was terminated by adding 1 ml of dinitrosalicylic acid reagent and heating in boiling water bath for 10 min.…”

Section: Enzyme Assaymentioning

confidence: 99%

Production and characterization of cellulolytic enzymes by isolated<br>Klebsiella sp. PRW-1 using agricultural waste biomass

Waghmare

Kshirsagar

Saratale

et al. 2014

Emir. J. Food Agric

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The efficient cellulolytic microorganism was isolated from soil samples collected from Shivaji University campus. Biochemical test and phylogenetic analysis of isolated culture identified as Klebsiella sp. PRW-1. The isolated culture could utilize pure cellulosic substrates (carboxymethylcellulose (CMC) and avicel) and different agricultural wastes like sugarcane bagasse, sugarcane barbojo, sorghum husks, grass powder, corn straw and paddy straw by producing a large amount of endoglucanase, exoglucanase, β-glucosidase, filter paperase (FPU), xylanase and glucoamylase. The reducing sugar production was found higher in the presence of grass powder and sugarcane barbojo. Effects of different physic-chemical parameters to achieve maximum cellulolytic enzymes production were systematically investigated. The effects of supplementation of different metals additives as well as the stability in the presence of higher temperature, pH and commercial detergents, on cellulolytic enzymes were also studied. The foregoing result increases the applicability of the strain for the utilization and bioconversion of lignocellulosic biomass that could be used for bioenergy production.

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Section: Enzyme Assaymentioning

confidence: 99%

Production and characterization of cellulolytic enzymes by isolated<br>Klebsiella sp. PRW-1 using agricultural waste biomass

Waghmare

Kshirsagar

Saratale

et al. 2014

Emir. J. Food Agric

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show abstract

“…Production of glucoamylase (GA) is performed mostly by two methods, Solid State or Solid-Substrate Fermentation (SSF) and Submerged Fermentation (SMF) [5]. SSF has many advantages over SMF, such as simple technique, superior productivity, low capital investment, low energy requirement and less water output, better product recovery and lack of foam build up and reported to be the most appropriate process for developing countries [6].…”

Section: Introductionmentioning

confidence: 99%

Use of Agro Industrial Residues for the Production of Amylase by Penicillium sp. for Applications in Food Industry

Arora¹,

Kaur²,

Kaur³

2017

J Biotechnol Biomater

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Glucoamylase production has been investigated by solid state fermentation using agro-industrial residues, rice bran, wheat bran and banana peel by Penicillium sp. Out of the different agricultural residues tested for enzyme production, rice bran yielded the maximum glucoamylase activity (10.29 ± 0.07 Units/ml), protein content (1.22 ± 0.0014 mg/ml) and specific activity (8.4 Units/mg protein) with Penicillium sp. after 7th day of fermentation as compared to other agro residues. Media supplementation with carbon and nitrogen sources enhances the enzyme activity. For maximum production of enzyme, sucrose (13.70 ± 0.77 Units/ml) (1% mass level) and yeast extract (14.41 ± 0.07Units/ml) (1% mass level) as carbon and nitrogen source respectively were found optimum on rice bran with Penicillium sp. Optimum enzyme activity was observed at 90°C, pH 9. The partial purification of enzyme from rice bran by Penicillium sp. was done with 60% ammonium sulphate precipitation showed maximum enzyme activity. Enzyme hydrolysis showed maximum activity (3.69 Units/ml) with potato starch from rice bran by Penicillium sp.

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“…Although glucoamylases have been produced by submerged fermentation traditionally, solid state fermentation (SSF) processes have been increasingly applied for the production of this enzyme in recent years (Ellaiah et al, 2002). SSF has advantages over submerged fermentation in that it is simpler, requires less capital, has superior productivity, lower energy requirement, requires simpler fermentation media, does not require rigorous control of fermentation parameters, uses less water, produces less waste water, allows for the easy control of bacterial contamination, and has a lower downstream processing cost (Ellaiah et al, 2002;Anto et al, 2006;Melikoglu et al, 2013a). However, the scale up of the SSF is a great challenge due to hardship of mixing, difficulty of heat removal and restricted water content which cause rapid change of moisture.…”

Section: Introductionmentioning

confidence: 99%

Glucoamylase production from food waste by solid state fermentation and its evaluation in the hydrolysis of domestic food waste

2014

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Glucoamylase production by solid-state fermentation using rice flake manufacturing waste products as substrate

Cited by 115 publications

References 12 publications

Production and characterization of cellulolytic enzymes by isolated<br>Klebsiella sp. PRW-1 using agricultural waste biomass

Production and characterization of cellulolytic enzymes by isolated<br>Klebsiella sp. PRW-1 using agricultural waste biomass

Use of Agro Industrial Residues for the Production of Amylase by Penicillium sp. for Applications in Food Industry

Glucoamylase production from food waste by solid state fermentation and its evaluation in the hydrolysis of domestic food waste

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