Production and characterization of cellulolytic enzymes by isolated<br>Klebsiella sp. PRW-1 using agricultural waste biomass

Abstract

The efficient cellulolytic microorganism was isolated from soil samples collected from Shivaji University campus. Biochemical test and phylogenetic analysis of isolated culture identified as Klebsiella sp. PRW-1. The isolated culture could utilize pure cellulosic substrates (carboxymethylcellulose (CMC) and avicel) and different agricultural wastes like sugarcane bagasse, sugarcane barbojo, sorghum husks, grass powder, corn straw and paddy straw by producing a large amount of endoglucanase, exoglucanase, β-glucosidase, filter paperase (FPU), xylanase and glucoamylase. The reducing sugar production was found higher in the presence of grass powder and sugarcane barbojo. Effects of different physic-chemical parameters to achieve maximum cellulolytic enzymes production were systematically investigated. The effects of supplementation of different metals additives as well as the stability in the presence of higher temperature, pH and commercial detergents, on cellulolytic enzymes were also studied. The foregoing result increases the applicability of the strain for the utilization and bioconversion of lignocellulosic biomass that could be used for bioenergy production.

Detergent‐compatible bacterial cellulases

“…They are routinely identified by adopting traditional and conventional methods such as morphological, cultural, microscopic, stain reaction, and taxonomic aspects as cleared indicated in the Bergey's Manual of Determinative Bacteriology . The identity is further verified and confirmed by molecular techniques such as 16S rRNA or 16S rDNA sequence analysis (Table ).…”

“…The detergent compatible cellulase‐producing bacteria are usually isolated from soil samples collected from various places . They are also isolated from rumen , hot spring water , and sediment (Table ).…”

“…To visualize clearly the hydrolysis zones, the culture Petri plates are flooded with Congo red solution and sodium chloride solution. The formation of clear zones around the bacterium is an indication cellulase secretion . Iodine solution along with mercuric iodide is also used to visualize cellulose hydrolysis.…”

“…The detergent compatible cellulases are generally produced by Bacillus species . The species of Fibrobacter , Klebsiella , Paenibacillus , Staphylotrichum , and Streptomyces were also reported as detergent compatible cellulase producers (Table ). Similarly, Bacillus species were reported by Ito as the chief producers of alkaline cellulases.…”

Expression and characterization of recombinant cellulases from an isolated Klebsiella

“…Klebsiella sp. PRW-1 could produce multiple cellulolytic and hemicellulolytic enzymes to hydrolyse different cellulosic substrates (Waghmare et al, 2014). Even though, the cellulases of Klebsiella have not been investigated extensively so far.…”