Glucoamylase immobilized on acid‐hydrolyzed starch graft polyacrylonitrile

Slininger, P. J.; Fanta, George F.; Abbott, Thomas P.

doi:10.1002/bit.260310802

Cited by 8 publications

(2 citation statements)

References 23 publications

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“…Carboxymethyl high amylose starch cross‐linked 35 (CM‐HASCL‐35) is suitable as carrier for immobilization of uricase 6. Acid hydrolyzed starch‐g‐polyacrylonitrile copolymers can bind and retain significant quantities of active glucoamylase 7. Glucose productivities up to 2.7 g/g carrier/hour were observed with the immobilized glucoamylase, and half‐lives up to 1800 h were indicative of activity longevity.…”

Section: Introductionmentioning

confidence: 99%

Response Surface Optimization of Acid Red 119 Dye Adsorption by Mixtures of Dried Sewage Sludge and Sewage Sludge Ash

Moghaddam

Arami

2012

CLEAN Soil Air Water

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The mixtures of dried sewage sludge (DSS) and sewage sludge ash were studied for removal of acid red 119 (AR119) dye as a new, more environmental friendly, and low cost adsorbent. For this purpose, response surface methodology was applied to optimize the dye removal efficiency and turbidity of treated dye solutions as two individual responses. Results revealed that an optimum condition under specified constraints (dye removal efficiency >95% and turbidity <50 NTU) was obtained at a contact time of 60 min, 40 wt% DSS in the mixture, an initial pH of 6, and an initial dye concentration of 200 mg dye/L in distilled water. Under the optimal condition, dye removal efficiency of 94.98% and effluent turbidity of 24.9 NTU was observed. In further studies, at optimum condition, the effect of some additives on adsorption process and desorption/reusability of adsorbent was investigated. It was observed that removal efficiency was significantly decreased to 83.76% when a simulated dye wastewater (containing the selected dye, acetic acid, and Glauber's salt dissolved in tap water) was used. Desorption studies revealed that AR119 dye could be well removed from dye‐loaded adsorbent by 0.3 M NaOH solution.

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Section: Introductionmentioning

confidence: 99%

Response Surface Optimization of Acid Red 119 Dye Adsorption by Mixtures of Dried Sewage Sludge and Sewage Sludge Ash

Moghaddam

Arami

2012

CLEAN Soil Air Water

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“…Although the immobilization of these enzymes is being intensively investigated (e.g. Vallat, 1983;Krakoviak et al, 1984;Ohtsuka et al, 1984;Kennedy et al, 1985;Szajani et al, 1985;Slininger et al, 1988), the scale-up of these processes is not economical except when used to obtain relatively high priced products. It is not there-fore worthwhile to extend them to impure substrates or those in low concentrations.…”

Section: Introductionmentioning

confidence: 99%

Enhancement of the bioproduction potential of an amylaceous effluent

Murado

González

Pastrana

et al. 1993

Bioresource Technology

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The treatment of starchy effluents could provide the basis for a series of simultaneous or alternative bioproductions easily integrable into a system of greater economic attraction that the simple production of SCP. In order to define some of the possible options, this study uses an amylaceous effluent for simultaneous production of SCP and an amylolytic preparation which is partially consumed in the saccharification of a parallel flow of the same effluent concentrated by ultrafiltration with cutoff at 100 kD. With regard to the development of the above system, this paper describes the problems associated with ultrafiltration, the conditions that optimize saccharification of the concentrate and the requirements of various possible bioproductions that could be obtained from the saccharified concentrate.

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Immobilization of urease on dialdehyde porous starch

Luo

2010

Starch Stärke

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Dialdehyde porous starch (DPS) was prepared by enzymatic hydrolysis and periodate oxidation. Urease was immobilized on DPS by several techniques including physical adsorption and covalent bonding. Optimum urease immobilization conditions for maximum enzyme activity were shown as following: immobilization pH 6.0, processing time of immobilization 25 h, immobilization temperature 25°C, aldehydic groups content 79.6% and ratio of supporter to urease 1:2 (w/v in g/mL). Temperature optimum of the free and immobilized urease were 60 and 70°C, respectively. The temperature curve of the immoblized urease was wider than that registered for the free urease. After being stored at ambient temperature for 42 days, the immobilized urease retained almost all of the original activity. DPS was found capable of holding the urease enzyme during a repeated cyclic test for ten times. An increase of the Km value for the immobilized urease was found. The foregoing data indicated that DPS is a fine supporter for urease and provided a basis for biosensor or bioreactor development with reduced costs and improved shelf life.

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Glucoamylase immobilized on acid‐hydrolyzed starch graft polyacrylonitrile

Cited by 8 publications

References 23 publications

Response Surface Optimization of Acid Red 119 Dye Adsorption by Mixtures of Dried Sewage Sludge and Sewage Sludge Ash

Response Surface Optimization of Acid Red 119 Dye Adsorption by Mixtures of Dried Sewage Sludge and Sewage Sludge Ash

Enhancement of the bioproduction potential of an amylaceous effluent

Immobilization of urease on dialdehyde porous starch

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