Glioblastoma multiforme in patients with history of extracranial cancer: Case series

Kalita, Ondřej; Trojanec, Radek; Megová, Magdalena Houdová; Hajdúch, Marián; Vaverka, Miroslav; Hrabálek, Lumir; Zlevorová, Miloslava; Drábek, Jir̆ı́; Tučková, Lucie; Vrbková, Jana

doi:10.1016/j.clineuro.2016.02.039

Cited by 3 publications

(1 citation statement)

References 24 publications

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“…Glioblastoma (GBM) is one of the most malignant of brain tumors (54% of all gliomas and 16% of all primary brain tumors [Stupp et al, 2005]), with an average life expectancy of ≤14 mo postdiagnosis (Ramirez et al, 2013; Urbańska et al, 2014; Williams, 2014; Kalita et al, 2016; Staller, 2016; Batash et al, 2017). One hallmark of GBM is the aggressive infiltration into healthy brain regions (Müller et al, 2014; Xie et al, 2014; Darmanis et al, 2017; Birch et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Human organotypic brain slice culture: a novel framework for environmental research in neuro-oncology

et al. 2019

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When it comes to the human brain, models that closely mimic in vivo conditions are lacking. Living neuronal tissue is the closest representation of the in vivo human brain outside of a living person. Here, we present a method that can be used to maintain therapeutically resected healthy neuronal tissue for prolonged periods without any discernible changes in tissue vitality, evidenced by immunohistochemistry, genetic expression, and electrophysiology. This method was then used to assess glioblastoma (GBM) progression in its natural environment by microinjection of patient-derived tumor cells into cultured sections. The result closely resembles the pattern of de novo tumor growth and invasion, drug therapy response, and cytokine environment. Reactive transformation of astrocytes, as an example of the cellular nonmalignant tumor environment, can be accurately simulated with transcriptional differences similar to those of astrocytes isolated from acute GBM specimens. In a nutshell, we present a simple method to study GBM in its physiological environment, from which valuable insights can be gained. This technique can lead to further advancements in neuroscience, neuro-oncology, and pharmacotherapy.

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