Ginsenoside Rg1 enhances the resistance of hematopoietic stem/progenitor cells to radiation-induced aging in mice

Cui, Chen; Mu, Xinyi; Zhou, Yue; Ke, Shun; Geng, Shan; Liu, Jun; Wang, Jianwei; Chen, Jie; Li, Tin-yu; Wang, Yaping

doi:10.1038/aps.2013.136

Cited by 45 publications

(43 citation statements)

References 32 publications

(37 reference statements)

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“…PSP can increase the spleen index, which indicates that PSP can enhance the immunity of the body. Aging-related beta-galactosidase staining is a well-recognized biological marker and reliable evidence for cell aging 20,21 . The results showed that the relative absorbance of senile beta-galactosidase-positive cells in renal tissue increased significantly compared with the Control group, indicating that the senile cells had senescence.…”

Section: Discussionmentioning

confidence: 99%

Protective effect of Polygonatum sibiricum Polysaccharide on D-galactose-induced aging rats model

Zheng

2020

Sci Rep

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The polysaccharide of Polygonatum sibiricum (PSP)is one of the main active ingredients of Polygonatum Polygonatum in Liliaceae. It has anti-tumor, anti-aging, immune regulation, and antioxidative effects. Recent studies have shown that the Klotho gene and fibroblast growth factor-23 (FGF-23) have a common receptor, which is closely related to aging and highly expressed in kidney and meninges. Our study aimed to investigate the anti-aging effect of PSP on D-galactose-induced rats and its mechanism. D-galactose (120 mg Kg -1 ) and PSP (100 mg Kg -1 ) was used to intervene in rats, respectively. Then The changes of indexes of the natural aging-like model rats before and after PSP intervention were observed. We found that PSP could significantly improve the learning and memory abilities of rats and reverse the pathological changes of kidney tissues in rats. At the same time, PSP up-regulated the expression of Klotho mRNA and Klotho protein in the renal cortex, down-regulated the expression of FOXO3a mRNA and p-FOXO3a protein in renal tissue, and inhibited the expression of FGF-23 protein in the femur. Our studies suggest that PSP may play a role by regulating the Klotho-FGF23 endocrine axis, alleviating oxidative stress, and balancing calcium and phosphorus metabolism.Aging should be an irreversible, comprehensive, and gradual loss of the function of each cell and organ tissue in the body. It is the body in the disease and function of the high-risk state of decline. The affecting factor of aging is not merely one, but rather a result of a combination of factors synergies. Modern medical studies have shown that the essence of aging refers to the irreversible process of gradual degeneration of structure and function after biological development and maturation under normal conditions, with the increase of age, the decline of self-function, the decline of internal environmental stability and stress ability, and the gradual degeneration of structure and function. With the development of modern genetic technology, the mechanism of aging has been studied in depth. It has become one of the research hotspots in recent years to search for active substances with an anti-aging effect in Nature by using the mechanism of aging.Klotho gene, which is an anti-aging gene, was first discovered by Kuro-o 1 in 1997, and then Xiao 2 , Kurosu 3 , Yamamoto 4 and others studied the gene and its expression. The results showed that the Klotho gene and fibroblast growth factor-23 (fibroblast growth factor-23) have common receptors, which are strictly related to aging and highly expressed in kidney and meninges.In January 2018, Chinese and American research teams published their research results in Nature. The crystal structure of the ternary complex of anti-aging protein alpha klotho-fibroblast growth factor receptor 1c (fibroblast growth factor receptor 1c) -fibroblast growth factor 23 (fibroblast growth factor 23) was analyzed, and the mechanism of its regulation of aging was elucidated 5 . The above studies and reports indicate that the Klot...

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Section: Discussionmentioning

confidence: 99%

Protective effect of Polygonatum sibiricum Polysaccharide on D-galactose-induced aging rats model

Zheng

2020

Sci Rep

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“…( 126 , 127 ). Two studies have shown that ginsenoside Rg1 provides neuroprotection against BBB disruption, edema formation, and neurological injury in rat models of cerebral ischemia/reperfusion through the downregulation of aquaporin 4 expression and anti-apoptosis pathways ( 38 , 128 ).…”

Section: Discussionmentioning

confidence: 99%

“…Ginsenoside Rb1 prevents homocysteine-induced endothelial dysfunction via PI3K/Akt activation and PKC inhibition ( 133 ). Ginsenoside Rg1 enhances angiogenesis after hypoxia ischemia brain damage in neonatal rats and in diabetic mice, in part through hypoxia-inducible factor (HIF-1a), glucocorticoid receptor (GR), and fibroblast growth factor receptor (VEGFR)-mediated pathways ( 134 – 138 ), and enhances the resistance of hematopoietic stem/progenitor cells to radiation-induced aging in mice ( 38 ). Notoginsenoside Ft1 promotes angiogenesis via HIF-1α mediated VEGF secretion and the regulation of PI3K/AKT and Raf/MEK/ERK signaling pathways ( 139 ).…”

Section: Discussionmentioning

confidence: 99%

“…P. notoginseng saponins extract (PNS) ameliorate learning and memory deficits in animals ( 20 – 26 ), probably by inhibiting oxidative stress and apoptosis and by stimulating neurogenesis ( 21 , 22 , 27 – 33 ). PNS is effective against ICH, transient focal ischemia, and cerebral infarction probably in part through improved brain blood circulation and energy metabolism ( 34 – 38 ). P. notoginseng has been used alone and as a key tonic ingredient in many other patent Chinese medicine for treatment of a variety of health conditions and has been proved to be effective in animal models of cerebral ischemia/reperfusion injury, arterial thrombosis, cardiovascular disorders, Alzheimer disease, diabetes and obesity, erectile dysfunction, neurodegeneration, neuroinflammation, oxidative stress, neurotoxicity, organ injury, and cancer ( 38 – 47 ).…”

Section: Introductionmentioning

confidence: 99%

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Efficacy and Safety of Panax notoginseng Saponin Therapy for Acute Intracerebral Hemorrhage, Meta-Analysis, and Mini Review of Potential Mechanisms of Action

Huang

et al. 2015

Front. Neurol.

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Intracranial/intracerebral hemorrhage (ICH) is a leading cause of death and disability in people with traumatic brain injury (TBI) and stroke. No proven drug is available for ICH. Panax notoginseng (total saponin extraction, PNS) is one of the most valuable herb medicines for stroke and cerebralvascular disorders in China. We searched for randomized controlled clinical trials (RCTs) involving PNS injection to treat cerebral hemorrhage for meta-analysis from various databases including the Chinese Stroke Trials Register, the trials register of the Cochrane Complementary Medicine Field, the Cochrane Central Register of Controlled Trials, MEDLINE, Chinese BioMedical disk, and China Doctorate/Master Dissertations Databases. The quality of the eligible trials was assessed by Jadad’s scale. Twenty (20) of the 24 identified randomized controlled trials matched the inclusive criteria including 984 ICH patients with PNS injection and 907 ICH patients with current treatment (CT). Compared to the CT groups, PNS-treated patients showed better outcomes in the effectiveness rate (ER), neurological deficit score, intracranial hematoma volume, intracerebral edema volume, Barthel index, the number of patients died, and incidence of adverse events. Conclusion: PNS injection is superior to CT for acute ICH. A review of the literature shows that PNS may exert multiple protective mechanisms against ICH-induced brain damage including hemostasis, anti-coagulation, anti-thromboembolism, cerebral vasodilation, invigorated blood dynamics, anti-inflammation, antioxidation, and anti-hyperglycemic effects. Since vitamin C and other brain cell activators (BCA) that are not considered common practice were also used as parts of the CT in several trials, potential PNS and BCA interactions could exist that may have made the effect of PNS therapy less or more impressive than by PNS therapy alone. Future PNS trials with and without the inclusion of such controversial BCAs as part of the CT could clarify the situation. As PNS has a long clinical track record in Asia, it could potentially become a therapy option to treat ICH in the US and Europe. Further clinical trials with better experimental design could determine the long-term effects of PNS treatment for TBI and stroke.

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“…Specifically, chronic systemic exposure of rodents to D-gal induces accelerated aging, including regression of bone marrow and the hematopoietic system that are similar to symptoms in natural aging [ 7 , 8 ]. In our previous study, we demonstrated that Rg1 possesses the capacity for anti-aging activity in HSCs both in vitro and vivo [ 9 ]. Although the aging of the hematopoietic microenvironment and BMSCs, as the supporting material of the HSCs, can also influence the hematopoietic function of bone marrow, there have been few reports concerning the anti-aging effects of Rg1 on the hematopoietic microenvironment and BMSCs.…”

Section: Introductionmentioning

confidence: 99%

The positive effects of Ginsenoside Rg1 upon the hematopoietic microenvironment in a D-Galactose-induced aged rat model

Jing

Wang

et al. 2015

BMC Complement Altern Med

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BackgroundGinsenoside Rg1 (Rg1) is one of the most active ingredients in Panax ginseng and has been proven to have anti-oxidative and anti-aging properties. However, there have been few reports concerning the anti-aging effects of Rg1 on the hematopoietic microenvironment and bone marrow stromal cells (BMSCs).MethodsThirty Sprague-Dawley rats were randomly divided into four groups (control, D-galactose (D-gal)-administration, Rg1-treatment, and D-gal-administration + Rg1-treatment groups). After D-gal and Rg1 treatment, BMSCs were extracted from femoral bone marrow for culture. After three passages, BMSCs were tested by senescence-associated β-galactosidase (SA-β-gal) staining, flow cytometric cell cycle phase distribution assay, CCK-8 cell proliferation assay, oxidative stress (reactive oxygen species [ROS], superoxide dismutase [SOD], and malondialdehyde [MDA]) assays, inflammatory marker (interleukin (IL)-2, IL-6, and tumor necrosis factor (TNF)-α) enzyme-linked immunosorbent assay (ELISA), stem cell factor (SCF) ELISA, and senescence-associated protein (p16, p21, and p53) Western blotting.ResultsCompared to the D-gal-administration group, the D-gal-administration + Rg1-treatment group showed significantly decreased levels of SA-β-gal + cell %, ROS, MDA, inflammatory marker expression, and senescence-associated protein expression as well as significantly increased levels of S-phase %, cell proliferation, SOD activity, and SCF expression. Compared to controls, the Rg-1-treatment group displayed significantly reduced levels of SA-β-gal + cell %, G1 phase %, ROS, MDA, inflammatory marker expression, senescence-associated protein expression, and SCF expression as well as significantly increased levels of S-phase %, cell proliferation, and SOD activity.ConclusionsRg1 improves the anti-aging ability of hematopoietic microenvironment through enhancing the anti-oxidant and anti-inflammatory capacities of BMSCs.

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Ginsenoside Rg1 enhances the resistance of hematopoietic stem/progenitor cells to radiation-induced aging in mice

Abstract: Administration of ginsenoside Rg1 enhances the resistance of HSC/HPCs to ionizing radiation-induced senescence in mice by inhibiting the oxidative stress reaction, reducing DNA damage, and regulating the cell cycle.

Cited by 45 publications

References 32 publications

Protective effect of Polygonatum sibiricum Polysaccharide on D-galactose-induced aging rats model

Protective effect of Polygonatum sibiricum Polysaccharide on D-galactose-induced aging rats model

Efficacy and Safety of Panax notoginseng Saponin Therapy for Acute Intracerebral Hemorrhage, Meta-Analysis, and Mini Review of Potential Mechanisms of Action

The positive effects of Ginsenoside Rg1 upon the hematopoietic microenvironment in a D-Galactose-induced aged rat model

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