Germinated and Ungerminated Seeds Extract from TwoLupinusSpecies: Biological Compounds Characterization and In Vitro and In Vivo Evaluations

Abstract

In recent years, nutraceuticals attracted a great amount of attention in the biomedical research due to their significant contribution as natural agents for prevention of various health issues. Ethanolic extracts from the ungerminated and germinated seeds of Lupinus albus L. and Lupinus angustifolius L. were analyzed for the content in isoflavones (genistein) and cinnamic acid derivatives. Additionally, the extracts were evaluated for antimicrobial, antiproliferative, and anti-inflammatory properties, using in vitro and in vivo tests. Germination proved to be a method of choice in increasing the amount of genistein and cinnamic acid derivatives in both Lupinus albus L. and Lupinus angustifolius L. seeds. Biological evaluation of all vegetal extracts revealed a weak therapeutic potential for both ungerminated and germinated seeds.

Total phenolic content, FTIR analysis, and antiproliferative evaluation of lupin seeds harvest from western Romania

“…Germination is a very good and accepted method used for the augmentation of the amount of the biological active substances [28]. In a similar approach, Andor et al, tested the antiproliferative activity of germinated and ungerminated extracts of lupines seeds against breast (MCF7, MDA-MB-231 cell lines) ovarian (A2780cell line), and cervical cancer (SiHa cell line), and described a similar decreased activity, with the observation that germination slightly increased the biological effect [4].…”

“…Some of the main phytochemicals present in the analyzed samples were established by means of HPLC in a previous study [4]. The targeted chemical compounds identified by HPLC were the isoflavone genistein, together with caffeic coumaric, ferulic, and rosmarinic acids.…”

“…Seeds where divided in two groups. One group was stored in standard conditions until analysis and the other group was subjected to germination, as previously described by Andor et al [4]. For both germinated and ungerminated seeds, extracts were prepared using 80% ethanol (FALC LCD series ultrasonic bath, for 10 min at 40 kHz and 28 °C).…”

“…Other chemical constituents worth mentioning, but present only in a concentration under 5%, include oleic and linoleic acids, flavonoids, isoflavonoids, carotenoids, polysaccharides, alkaloids. The amount of the above-mentioned constituents can vary depending on the environment, pedoclimatic and genetic conditions [4][5][6]. It is also very important to mention that from the point of view of the amount of protein, lupine seeds follow closely soybean seeds, and as a particular advantage, Lupinus spp.…”

Antimicrobial Activity and Cytotoxic Effect on Gingival Cells of Silver Nanoparticles Obtained by Biosynthesis

“…administration of low doses was observed [12]. Plants are an endless source of compounds with remarkable biological activity [13]. Application of biosynthesis in the case of silver nanoparticles with various pharmacological properties are possible due to the resources from the natural environment and presents a number of advantages, among which: environment friendly, economical effective and easy production [14].…”