Total phenolic content, FTIR analysis, and antiproliferative  evaluation of lupin seeds harvest from western Romania

Danciu, Corina; Pavel, Ioana Zinuca; Băbuţa, Roxana; Alexa, Ersilia; Pop, Georgeta; Şoica, Codruţa; Dehelean, Cristina; Radulov, Isidora; Suciu, Oana

doi:10.26444/aaem/80795

Cited by 11 publications

(8 citation statements)

References 25 publications

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“…Our results are comparable also with TPC values 4360-7250 mg GAE/kg DM determined by [42]. e average TPC in seeds of investigated lupin cultivars 5000 mg GAE/kg DM was comparable with results of [45] but higher than total phenolic content 2839 mg GAE/kg DM reported by [46]. e highest TPC was determined in cv.…”

Section: Resultssupporting

confidence: 89%

“…Siger et al [38] determined in two cultivars of white lupin seeds higher content of p-hydroxybenzoic acid (22.77 and 27.82 mg/kg) but lower content of caffeic acid (0.58 and 0.09 mg/kg) as well as p-coumaric acid (0.11 and 0.18 mg/kg) compared to our results. e presence of genistein and main cinnamic acids derivatives (ferulic, caffeic, rosmarinic, and coumaric acids) in germinated and also in ungerminated lupin seeds was confirmed by [45] using FTIR spectra. Many studies were focused on phenolics composition of other kinds of legumes.…”

Section: Resultsmentioning

confidence: 91%

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White Lupin as a Promising Source of Antioxidant Phenolics for Functional Food Production

Vollmannová

Lidiková

Musilová

et al. 2021

Journal of Food Quality

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Although white lupin is the oldest known legume in the history, it has been forgotten for many years. Now, the interest of food producers concerning white lupin is increased again. The aim of this study was to evaluate the total phenolic content (TPC), antioxidant activity (AA), and the content of selected phenolics in 11 white lupin cultivars. The determined TPC was in the interval 4260–5663 mg GAE/kg DM and the values of AA determined using DPPH•, ABTS•+, and FRAP methods were in the ranges 0.993–1.878, 5.496–7.924, and 1.328–1.741 μmol TE/g DM, respectively. Individual phenolics content (4-hydroxybenzoic acid, caffeic acid, trans-p-coumaric acid, trans-ferulic acid, myricetin, quercetin, apigenin, and genistein) were determined, too. Caffeic acid (442.9–766.2 mg/kg DM) and myricetin (11.2–21.2 mg/kg DM) are the dominant phenolics in the investigated lupin cultivars. Statistically significant differences in all investigated variables were observed between the tested cultivars except for quercetin. The obtained results show that the Astra and Nelly cultivars are a rich source of phenolic acids.

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Section: Resultssupporting

confidence: 89%

Section: Resultsmentioning

confidence: 91%

White Lupin as a Promising Source of Antioxidant Phenolics for Functional Food Production

Vollmannová

Lidiková

Musilová

et al. 2021

Journal of Food Quality

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“…Dichrostachys cinerea water and ethanolic extracts had similar spectra, having 15 major peaks, among which some presented higher absorption intensities in the ethanolic extract. The intense absorption peak at 3275 cm −1 , which was one of the peaks with higher intensity in the ethanolic extract, could be attributed to the hydroxyl group specific for phenolics and phenolic glycosides [ 30 , 31 ]. The next bands at 2927 cm −1 and 2852 can be assigned to the alkane –CH bond [ 30 , 31 ].…”

Section: Resultsmentioning

confidence: 99%

“…The intense absorption peak at 3275 cm −1 , which was one of the peaks with higher intensity in the ethanolic extract, could be attributed to the hydroxyl group specific for phenolics and phenolic glycosides [ 30 , 31 ]. The next bands at 2927 cm −1 and 2852 can be assigned to the alkane –CH bond [ 30 , 31 ]. The medium intensity band identified only in the methanolic extract at 1732 cm −1 and 1610 cm −1 could be attributed to could be assigned to the C=O stretching vibration in the lipid esters [ 32 ].…”

Section: Resultsmentioning

confidence: 99%

Polyphenols from Dichrostachys cinerea Fruits Anti-Inflammatory, Analgesic, and Antioxidant Capacity in Freund’s Adjuvant-Induced Arthritic Rat Model

et al. 2022

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Dichrostachys cinerea (L.) Wigth & Arn. (DC) is widely used in traditional medicine against several inflammatory diseases, especially rheumatoid arthritis, because of its antioxidant and anti-inflammatory effects. This study aimed to characterize the polyphenol-rich DC fruit extracts and investigate the analgesic, anti-inflammatory, and antioxidant effects in a rat inflammation model induced by complete Freund’s adjuvant (CFA). Water and ethanolic extracts were characterized using liquid chromatography coupled with mass spectrometry (LC-MS), Fourier-transform infrared (FTIR) spectroscopy, and gas chromatography coupled with mass spectrometry (GC-MS). The polyphenol-rich extracts were administered in three different concentrations for 30 days. Pain threshold, thermal hyperalgesia, edema, and serum biomarkers specific to inflammatory processes or oxidative stress were evaluated. Both extracts were rich in polyphenolic compounds, mainly flavan-3-ols, proanthocyanidins, and flavone glycosides, which had important in vitro antioxidant capacity. DC fruit extracts administration had the maximum antinociceptive and anti-inflammatory effects after one day since the CFA injection and showed promising results for long-term use as well. The measurement of pro-inflammatory cytokines, cortisol, and oxidative stress parameters showed that DC extracts significantly reduced these parameters, being dose and extract-type dependent. These results showed potential anti-inflammatory, analgesic, and antioxidative properties and revealed the necessity of using a standardized polyphenolic DC extract to avoid result variability.

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“…To evaluate the regressive effect of Pg on the invasion capacity of A549 lung adenocarcinoma cells, the scratch test was performed [ 35 ]. A number of 2 × 10 5 cells/well were seeded onto 12-well culture plates.…”

Section: Methodsmentioning

confidence: 99%

Inorganic Element Determination of Romanian Populus nigra L. Buds Extract and In Vitro Antiproliferative and Pro-Apoptotic Evaluation on A549 Human Lung Cancer Cell Line

et al. 2021

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Populus nigra L. is a plant from Salicaceae family, native in Europe. Many parts of this tree can be used as active ingredients, but the most valuable are the buds. In recent years, a growing number of studies reported their activity in the development of a wide range of pharmacological activities including diabetes, cardiovascular diseases, and cancer. The aim of this study was to determine the phytochemical composition and to evaluate the inorganic elements’ concentration as well as the in vitro antiproliferative and pro-apoptotic potential of a Populus nigra L. buds extract collected from Timișoara (Romania) against A549 human lung cancer cell line. Populus nigra L. bud extract was found to contain twelve different phenolic compounds. The inorganic elements concentrations were below the limit of detection for Co, Pb, and As, whereas Cu = 6.66 µg/g; Cr = 0.79 µg/g; Ni = 3.28 µg/g; Fe = 39.00 µg/g; Zn = 14.84 µg/g; Mn = 0.59 µg/g; Al = 2109.87 µg/g; and Cd = 0.019 µg/g. The extract was tested for the in vitro antiproliferative and pro-apoptotic potential on A549 human lung cancer cell line using different concentrations, namely 10, 25, 50, 75, 100, and 150 μg/mL. Results have shown that poplar bud extract induced a significant decrease of tumor cell viability in a dose-dependent manner with an IC50 = 72.49 μg/mL and blocked the cells in the G0/G1 phase of the cell cycle. Phenomena of early apoptosis (from 1.34 ± 0.33% control cells to 2.68 ± 0.62% at 150 µg/mL) and late apoptosis (from 1.43 ± 0.14% control cells to 5.15 ± 1.02% at 150 µg/mL) were detected by Annexin V-PI double staining. Poplar bud extract can be regarded as a promising candidate for future studies involving lung cancer.

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Total phenolic content, FTIR analysis, and antiproliferative evaluation of lupin seeds harvest from western Romania

Cited by 11 publications

References 25 publications

White Lupin as a Promising Source of Antioxidant Phenolics for Functional Food Production

White Lupin as a Promising Source of Antioxidant Phenolics for Functional Food Production

Polyphenols from Dichrostachys cinerea Fruits Anti-Inflammatory, Analgesic, and Antioxidant Capacity in Freund’s Adjuvant-Induced Arthritic Rat Model

Inorganic Element Determination of Romanian Populus nigra L. Buds Extract and In Vitro Antiproliferative and Pro-Apoptotic Evaluation on A549 Human Lung Cancer Cell Line

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