Antimicrobial Activity and Cytotoxic Effect on Gingival Cells of Silver Nanoparticles Obtained by Biosynthesis

Andor, Bogdan; Tischer, Alina Andreea; Berceanu-Văduva, Delia; Lăzureanu, Voichiţa Elena; Cheveresan, Adelina; Poenaru, Mărioara

doi:10.37358/rc.19.3.7006

Cited by 8 publications

(8 citation statements)

References 11 publications

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“…To study cell viability, the colorimetric microculture tetrazolium assay (MTT) was used, as described by Andor et al ( 23 ) and Isaia et al ( 24 ). Cells were cultured in 96-well plates using a number of 1×10 4 cells/well.…”

Section: Methodsmentioning

confidence: 99%

Mechanistic investigations of antitumor activity of a Rhodamine B‑oleanolic acid derivative bioconjugate

et al. 2020

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Cancer remains a major health problem worldwide due to its high mortality rate. New therapeutic options highlight the importance of discovering new compounds that target the tumor microenvironment, interrupt angiogenesis and act selectively. The present study assessed the antitumor effect and investigated the mechanism of action of a rhodamine B-conjugated oleanolic acid derivative (RhodOA). Consequently, the compound was tested on different human tumor cell lines (A375 melanoma, A549 lung adenocarcinoma and MDA-MB-231 breast adenocarcinoma) and on a non-tumor cell line HaCaT human keratinocyte. RhodOA produced a dose-dependent decrease in tumor cell viability especially in the melanoma cells while affecting the keratinocytes less. In melanoma cells, RhodOA reduced cell migration and produced condensation of cell nuclei and of actin fibers. Furthermore, an impairment in melanoma cell mitochondrial function was observed, while the mitochondrial function of keratinocytes was left intact. In the in ovo chorioallantoic membrane model, RhodOA elicited antiangiogenic effect, without showing irritation effect on the membrane. The study provides information on the selective antitumor effect of the derivative and its ability to inhibit cellular respiration, therefore RhodOA can be classified as 'MITOCAN'.

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Section: Methodsmentioning

confidence: 99%

Mechanistic investigations of antitumor activity of a Rhodamine B‑oleanolic acid derivative bioconjugate

et al. 2020

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“…Similarly, risk factors such as lifestyle (nutrition, smoking, alcohol consumption), long-term use of contraceptives, exposure to environmental toxicants were described in the tumorigenesis related to human papilloma virus infection ( 35 , 36 ). A novel identified risk factor for colorectal cancer is microbial dysbiosis (biofilms) in gut microbiome ( 37 ), biofilms being also responsible for most types of infections (~65% nosocomial infections and ~80% of all microbial infections) ( 38 ). Furthermore, environmental toxicants such as heavy metals, including cadmium, play an asserted role in the pathogenesis of the disease, according to recent studies ( 14 , 39 ).…”

Section: Discussionmentioning

confidence: 99%

Exposure to cadmium and copper triggers cytotoxic effects and epigenetic changes in human colorectal carcinoma HT‑29 cells

et al. 2020

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Recent scientific evidence suggests a link between epigenetic changes (DNA methylation) and tumorigenesis. Moreover, a potential carcinogenic mechanism of cadmium was associated with changes in DNA methylation. In this study we investigated the impact of CdCl 2 and CuSO 4 aqueous solutions on DNA methylation in HT-29 cells by quantifying DNA methyltransferase (DNMT1, DNMT3A and DNMT3B) mRNA expression. Furthermore, we also studied the cytotoxic and anti-migratory potential of these substances. The results showed a dose-dependent decrease of viable cell percentage following 24 h of exposure (at concentrations of 0.05; 0.2; 1; 10 and 100 µg/ml), and an inhibitory effect on HT-29 cell migration capacity. In addition, RT-qPCR results showed that cadmium acts as a hypomethylating agent by suppressing DNMT expression, whereas copper acts as a hypermethylating compound by increasing DNMT expression. These findings suggest a cytotoxic potential of both cadmium and copper on HT-29 cells and their capacity to induce epigenetic changes.

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“…The reagents used in the present study were of analytical grade, acquired from Sigma Aldrich, Germany and utilized as received without any other supplementary purification. Green silver nanoparticles were obtained as previously described by Andor et al [8]. Briefly, to an aqueous rosemary leaves extract (RAE) -recognized both as a reducing and stabilizing agent -was added an aqueous solution of AgNO 3 (1 mM) and the reaction mixture was inserted in an orbital shaker (away from light, for two days, 250 rpm, at room temperature), then purified and utilized in the in vitro experiments.…”

Section: Experimental Part Materials and Methodsmentioning

confidence: 99%

Antimicrobial Activity of Biosynthesized Silver Nanoparticles Compared to Standard Antibiotics Used in ORL Infections

Tischer¹,

Vaduva²,

Balica³

et al. 2019

Rev. Chim.

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In recent years, bacterial infections in hospitals have grown particularly due to the development of antibiotic resistance. Recent research targets the discovery of new antibiotics that exhibit broad spectrum of action without adverse effects or minimizing adverse effects. In this study, the activity of biosynthesized silver nanoparticles against three bacteria commonly found in infectious diseases in the ORL sphere was evaluated. The recorded data revealed an activity comparable to that of the standard antibiotics used in these types of infections, with the observation that the activity of the nanoparticles could also be observed in the particular cases of antibiotic resistance.

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Antimicrobial Activity and Cytotoxic Effect on Gingival Cells of Silver Nanoparticles Obtained by Biosynthesis

Cited by 8 publications

References 11 publications

Mechanistic investigations of antitumor activity of a Rhodamine B‑oleanolic acid derivative bioconjugate

Mechanistic investigations of antitumor activity of a Rhodamine B‑oleanolic acid derivative bioconjugate

Exposure to cadmium and copper triggers cytotoxic effects and epigenetic changes in human colorectal carcinoma HT‑29 cells

Antimicrobial Activity of Biosynthesized Silver Nanoparticles Compared to Standard Antibiotics Used in ORL Infections

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