Gentechnik bei Pflanzen

Kempken, Renate; Kempken, Frank

doi:10.1007/978-3-662-07434-3

Cited by 6 publications

(5 citation statements)

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“…However, very little is yet known about the mechanism and biochemistry of mitochondrial RNA editing , largely due to the lack of a transformation system for plant mitochondria and the limitations of existing in vitro systems (Araya et al 1992;Blanc et al 1995;Yu and Schuster 1995). We have adopted an alternative procedure to address the problem, using electroporation to introduce genes into purified plant mitochondria and then following the processing of newly synthesized transcripts in organello (Kempken et al 2000). Circular or linear DNAs of up to 11 kb in size were successfully introduced into mitochondria purified from Zea mays and Sorghum bicolor.…”

Section: Introductionmentioning

confidence: 99%

Electroporation of isolated higher-plant mitochondria: transcripts of an introduced cox2 gene, but not an atp6 gene, are edited in organello

Staudinger

Kempken

2003

Mol Gen Genomics

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To facilitate the analysis of RNA processing in plant mitochondria, a method was established for introducing foreign DNA into mitochondria isolated from maize and sorghum. This method permits the uptake of DNA of up to 11 kb into the mitochondrial matrix. In vitro incubation of maize mitochondria in a specific buffer system was found to permit splicing and editing of newly synthesized RNAs for a period of at least 7 h. This was shown both for transcripts of endogenous mitochondrial genes (atp6, cox2) and for transcripts derived from an introduced Arabidopsis thaliana cox2 gene. In contrast, when a Sorghum bicolor atp6 gene was introduced into isolated maize mitochondria, the gene was transcribed, but the RNA was not edited, although all the editing sites in maize and sorghum atp6 RNA are identical. This may indicate the presence of transcript-specific cis -acting regions in the up- or downstream untranslated sequences of the mRNA. The system described here should allow further dissection of the mechanism of RNA editing in plant mitochondria.

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Section: Introductionmentioning

confidence: 99%

Electroporation of isolated higher-plant mitochondria: transcripts of an introduced cox2 gene, but not an atp6 gene, are edited in organello

Staudinger

Kempken

2003

Mol Gen Genomics

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“…As a first step to elucidate the mechanism of RNA editing, experimental systems for electroporation of mitochondria and in organello incubation [11][12][13] as well as for in vitro RNA editing [14,15] have recently been established. So far, these approaches have yielded information on the cis-acting sequences required for the recognition of mitochondrial RNA editing sites, e.g.…”

Section: Introductionmentioning

confidence: 99%

Mono‐ and dicotyledonous plant‐specific RNA editing sites are correctly edited in both in organello systems

Bolle

Kempken

2006

FEBS Letters

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We set out to analyse the phylogenetic distribution of cox2 RNA editing sites. Database searches have revealed the presence of mono-and dicotyledonous-specific RNA editing sites. Therefore, to better understand tRNA editing system in plants, we developed a new dicotyledonous in organello RNA editing system using cauliflower mitochondria and analysed the transcription of the cox2 gene for both maize and Arabidopsis. These results were compared with those obtained from a maize mitochondrial in organello system. Surprisingly, both the monoand dicotyledonous cox2 transcripts were efficiently edited in the mitochondrial cauliflower and maize in organello systems, respectively, even for RNA editing sites not present in the endogenous cox2 sequences. Taken together, our observations support a self-guiding-transcript model for RNA editing in higher plants.

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“…has been reported with use of commercial cellulose as a standard to compare the release of glucose monomers from untreated straw with that from straw pretreated with Pleurotus solid-state fermentation. In that study, lignin content decreased by 51% during the incubation period (90 days) (Kempken, 2013). Also, sugarcane is an excellent substrate for the cultivation of Pleurotus species.…”

Section: Resultsmentioning

confidence: 77%

Green potential ofPleurotusspp. in biotechnology

Секан

Myronycheva

Karlsson

et al. 2019

PeerJ

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Background The genus Pleurotus is most exploitable xylotrophic fungi, with valuable biotechnological, medical, and nutritional properties. The relevant features of the representatives of this genus to provide attractive low-cost industrial tools have been reported in numerous studies to resolve the pressure of ecological issues. Additionally, a number of Pleurotus species are highly adaptive, do not require any special conditions for growth, and possess specific resistance to contaminating diseases and pests. The unique properties of Pleurotus species widely used in many environmental technologies, such as organic solid waste recycling, chemical pollutant degradation, and bioethanol production. Methodology The literature study encompasses peer-reviewed journals identified by systematic searches of electronic databases such as Google Scholar, NCBI, Springer, ResearchGate, ScienceDirect, and ISI Web of Knowledge. The search scheme was divided into several steps, as described below. Results In this review, we describe studies examining the biotechnological feasibility of Pleurotus spp. to elucidate the importance of this genus for use in green technology. Here, we review areas of application of the genus Pleurotus as a prospective biotechnological tool. Conclusion The incomplete description of some fungal biochemical pathways emphasises the future research goals for this fungal culture.

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Gentechnik bei Pflanzen

Cited by 6 publications

References 0 publications

Electroporation of isolated higher-plant mitochondria: transcripts of an introduced cox2 gene, but not an atp6 gene, are edited in organello

Electroporation of isolated higher-plant mitochondria: transcripts of an introduced cox2 gene, but not an atp6 gene, are edited in organello

Mono‐ and dicotyledonous plant‐specific RNA editing sites are correctly edited in both in organello systems

Green potential ofPleurotusspp. in biotechnology

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