Genotyping of Asparagus (Asparagus Officinalis L.) Cultivars With SSR Markers

Takeuchi, Yoko; Sakaguchi, Yuichi; Okubo, Hiroshi

doi:10.17660/actahortic.2012.950.17

Cited by 3 publications

(8 citation statements)

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“…1), while the other progeny had one peak or two peaks with similar RFI and segregated into four (AG7), two (AG10), or three (TC1) genotypes (Table 1), all in accordance with Mendelian expectations (Takeuchi et al, 2012). The genotype 174/174/178 at the AG7 locus in 07M-61 is composed of one 174 from the female, another 174 from the male, and 178 from the female, excluding a 172 gene from the male parent, since the two alleles (151/151) were from the seed parent in AG2.…”

Section: Resultssupporting

confidence: 68%

“…The other genotypes were also identified in a similar manner. It was also confirmed that the segregation of the progeny genotypes in which data for 07M-61 were excluded fitted with monogenic inheritance by χ 2 -test (Takeuchi et al, 2012).…”

Section: Morphological Characteristicsmentioning

confidence: 53%

“…Table 1 shows the SSR genotypes of the parents and the segregation of their progeny in AG2, AG3, AG7, AG10, and TC1 loci. The genotype of the plant was identified as 147/151 in AG2 locus when two peaks representing 147 and 151 showed a similar relative fluorescent intensity (RFI) with each other as previously reported (Takeuchi et al, 2012). The other genotypes were also identified in a similar manner.…”

Section: Morphological Characteristicsmentioning

confidence: 55%

“…The mixtures were denatured (95°C) for 2 min and then cooled on ice immediately. Genotyping was conducted by using the capillary sequencer (ABI PRISM 310 Genetic Analyzer; Applied Biosystems) and fragment sizes were determined by using GeneScan version 3.7 (Applied Biosystems), as reported previously (Takeuchi et al, 2012). Each SSR was tested for single-locus Mendelian segregation of crossed progenies using chi-square goodness-of-fit test.…”

Section: Ssr Analysismentioning

confidence: 99%

“…SSR markers have been established from expressed sequence tag (EST) in asparagus (Caruso et al, 2008). We performed 2x × 2x crosses in order to evaluate the applicability of SSR as genetic markers in asparagus, and showed that it is useful for hybrid confirmation and for evaluating the genetic purity of clonal hybrid cultivars, as well as for the genotyping of triploid and tetraploid cultivars (Takeuchi et al, 2012). We report here the occurrence of a triploid asparagus plant from a diploid-diploid cross and its origin determined by analyzing SSR markers.…”

Section: Introductionmentioning

confidence: 99%

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Occurrence of a Spontaneous Triploid Progeny from Crosses between Diploid Asparagus (Asparagus officinalis L.) Plants and Its Origin Determined by SSR Markers

Takeuchi

Iwato

Sakazono

et al. 2014

J. Japan. Soc. Hort. Sci.

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The origin of a spontaneous triploid asparagus plant from crosses of 2x × 2x was investigated by SSR and flow cytometric analyses. One hundred and twenty-four progeny were obtained from crosses between a diploid female 'Gold Schatz' and a diploid male 'Hokkai 100'. SSR analysis proved that two and one genes were transmitted from the maternal and paternal parents, respectively, at each SSR locus of one progeny, 07M-61, whereas one gene each was from the female and male parents in the other diploid progeny. Triploidy of 07M-61 was confirmed by flow cytometric analysis. It was suggested that the triploid plant was derived from fertilization between an unreduced egg and reduced sperm nuclei, given its SSR genotypes. It was also suggested that the unreduced maternal gamete was derived from first division restitution (FDR) or second division restitution (SDR) with chiasma occurrence during meiosis. There were no noticeable morphological differences between the triploid and diploid progeny.

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Section: Resultssupporting

confidence: 68%

Section: Morphological Characteristicsmentioning

confidence: 53%

Section: Morphological Characteristicsmentioning

confidence: 55%

Section: Ssr Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Occurrence of a Spontaneous Triploid Progeny from Crosses between Diploid Asparagus (Asparagus officinalis L.) Plants and Its Origin Determined by SSR Markers

Takeuchi

Iwato

Sakazono

et al. 2014

J. Japan. Soc. Hort. Sci.

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Origin of polyembryonic seeds and production of haploids in asparagus

Takeuchi¹,

Kosaza²,

Tomiyoshi³

et al. 2020

Acta Hortic.

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New hybrids between cultivated and wild species of asparagus (Asparagus spp.) and their validation by SSR markers

Ranjbar¹,

Ghahremani²,

Mousavizadeh³

et al. 2022

Europ.J.Hortic.Sci.

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Garden asparagus ( L., 2n =2x = 20) is an important vegetable crop cultivated worldwide. A narrow genetic base has been pointed out in the current cultivars. Wild related asparagus species could be a valuable resource to extend genetic variation of this crop, but their use have been scarce between cultivated and wild asparagus species, A. persicus (2x) and A. breslerianus (8x), were obtained by hand-pollination. The genetic origin of hybrid plants loid hybrids between cultivated asparagus and both A. persicus and a wild accession of were detected by SSR markers in progenies obtained from open pollination. In this case, the genetic polymorphism among 21 asparagus accessions belong to 10 dioecious species ( (2x, 4x, 8x, 10x), A. verticillatus (2x), A. breslerianus (8x), A. brachyphyllus (6x), A. pseudoscaber (6x), A. acutifolius (4x), A. persicus (2x), A. macrorrhizus (12x), A. maritimus (6x) and A. prostratus (4x open pollination condition were analyzed by using 26 amplicon were found among the accessions. The utility rate of this information for detecting of hybrid plants was tested by employing progenies from the diploid species included A. persicus, wild accession of A. ofand A. verticillatus. Fifteen and nine hybrid plants were detected in progenies from A. persicus and wild accession of respectively, whereas no

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Genotyping of Asparagus (Asparagus Officinalis L.) Cultivars With SSR Markers

Cited by 3 publications

References 0 publications

Occurrence of a Spontaneous Triploid Progeny from Crosses between Diploid Asparagus (Asparagus officinalis L.) Plants and Its Origin Determined by SSR Markers

Occurrence of a Spontaneous Triploid Progeny from Crosses between Diploid Asparagus (Asparagus officinalis L.) Plants and Its Origin Determined by SSR Markers

Origin of polyembryonic seeds and production of haploids in asparagus

New hybrids between cultivated and wild species of asparagus (Asparagus spp.) and their validation by SSR markers

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