New hybrids between cultivated and wild species of asparagus (Asparagus spp.) and their validation by SSR markers

Abstract

Garden asparagus ( L., 2n =2x = 20) is an important vegetable crop cultivated worldwide. A narrow genetic base has been pointed out in the current cultivars. Wild related asparagus species could be a valuable resource to extend genetic variation of this crop, but their use have been scarce between cultivated and wild asparagus species, A. persicus (2x) and A. breslerianus (8x), were obtained by hand-pollination. The genetic origin of hybrid plants loid hybrids between cultivated asparagus and both A. persicus and a wild accession of were detected by SSR markers in progenies obtained from open pollination. In this case, the genetic polymorphism among 21 asparagus accessions belong to 10 dioecious species ( (2x, 4x, 8x, 10x), A. verticillatus (2x), A. breslerianus (8x), A. brachyphyllus (6x), A. pseudoscaber (6x), A. acutifolius (4x), A. persicus (2x), A. macrorrhizus (12x), A. maritimus (6x) and A. prostratus (4x open pollination condition were analyzed by using 26 amplicon were found among the accessions. The utility rate of this information for detecting of hybrid plants was tested by employing progenies from the diploid species included A. persicus, wild accession of A. ofand A. verticillatus. Fifteen and nine hybrid plants were detected in progenies from A. persicus and wild accession of respectively, whereas no

The garden asparagus (Asparagus officinalis L.) mitochondrial genome revealed rich sequence variation throughout whole sequencing data

“…At present, the main commercial asparagus varieties are diploid varieties that originate from the original “Violet Dutch.” ( Knaflewski, 1996 ). It has been demonstrated that asparagus germplasm resources have a narrow genetic base ( Nothnagel et al., 2012 ; Amian et al., 2018 ; Mousavizadeh et al., 2021 ; Ranjbar et al., 2022 ). Therefore, expanding the genetic diversity is a prominent goal in asparagus breeding.…”

DataSheet_1.xlsx

“…(Knaflewski, 1996). It has been demonstrated that asparagus germplasm resources have a narrow genetic base (Nothnagel et al, 2012;Amian et al, 2018;Mousavizadeh et al, 2021;Ranjbar et al, 2022). Therefore, expanding the genetic diversity is a prominent goal in asparagus breeding.…”