Occurrence of a Spontaneous Triploid Progeny from Crosses between Diploid Asparagus (Asparagus officinalis L.) Plants and Its Origin Determined by SSR Markers

Takeuchi, Yoko; Iwato, Miyuki; Sakazono, Satomi; Okubo, Hiroshi

doi:10.2503/jjshs1.ch-073

Cited by 4 publications

(3 citation statements)

References 21 publications

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“…Interestingly, in the frame of a breeding program in Annona an unexpected high proportion of triploid genotypes was found in the progeny of an interspecific cross involving the diploid species, A. cherimola and A. squamosa (Martin, 2013). Spontaneous triploids from crosses involving diploid parents have been reported in intra- and inter-specific crosses in several genera such as Citrus (Esen and Soost, 1971), Populus (Bradshaw and Stettler, 1993), Anthoxanthum (Bretagnolle, 2001), Rhododendron (Ureshino and Miyajima, 2002), Rosa (Crespel et al, 2006), and Asparagus (Ozaki et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Polyploidy in Fruit Tree Crops of the Genus Annona (Annonaceae)

et al. 2019

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Genome duplication or polyploidy is one of the main factors of speciation in plants. It is especially frequent in hybrids and very valuable in many crops. The genus Annona belongs to the Annonaceae, a family that includes several fruit tree crops, such as cherimoya (Annona cherimola), sugar apple (Annona squamosa), their hybrid atemoya (A. cherimola × A. squamosa) or pawpaw (Asimina triloba). In this work, genome content was evaluated in several Annona species, A. triloba and atemoya. Surprisingly, while the hybrid atemoya has been reported as diploid, flow cytometry analysis of a progeny obtained from an interspecific cross between A. cherimola and A. squamosa showed an unusual ploidy variability that was also confirmed karyotype analysis. While the progeny from intraspecific crosses of A. cherimola showed polyploid genotypes that ranged from 2.5 to 33%, the hybrid atemoyas from the interspecific cross showed 35% of triploids from a total of 186 genotypes analyzed. With the aim of understanding the possible implications of the production of non-reduced gametes, pollen performance, pollen size and frequency distribution of pollen grains was quantified in the progeny of this cross and the parents. A large polymorphism in pollen grain size was found within the interspecific progeny with higher production of unreduced pollen in triploids (38%) than in diploids (29%). Moreover, using PCR amplification of selected microsatellite loci, while 13.7% of the pollen grains from the diploids showed two alleles, 41.28% of the grains from the triploids amplified two alleles and 5.63% showed up to three alleles. This suggests that the larger pollen grains could correspond to diploid and, in a lower frequency, to triploid pollen. Pollen performance was also affected with lower pollen germination in the hybrid triploids than in both diploid parents. The results confirm a higher percentage of polyploids in the interspecific cross, affecting pollen grain size and pollen performance. The occurrence of unreduced gametes in A. cherimola, A. squamosa and their interspecific progeny that may result in abnormalities of ploidy such as the triploids and tetraploids observed in this study, opens an interesting opportunity to study polyploidy in Annonaceae.

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Section: Introductionmentioning

confidence: 99%

Polyploidy in Fruit Tree Crops of the Genus Annona (Annonaceae)

et al. 2019

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“…Ozaki et al . [ 40 ] discovered a spontaneous triploid asparagus plant from crosses with diploid parents. For the accessions A .…”

Section: Discussionmentioning

confidence: 99%

“…In the case of Asparagus , a high correlation has so far been found between the ploidy level of A . officinalis cultivars and their genome size [ 40 ]. Such as a connection between total chromosome length and total chromosome volume was described [ 47 ].…”

Section: Discussionmentioning

confidence: 99%

Karyological and nuclear DNA content variation of the genus Asparagus

2022

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Asparagus wild relatives could be a promising possibility to extent the genetic variability of garden asparagus and for new cultivars with favorable traits such as high yield stability, disease resistance and stress tolerance. In order to achieve an efficient use in breeding, a detailed cytogenetic characterization of the accessions is necessary. This study worked on 35 Asparagus accessions, including A. officinalis cultivars (‘Darlise’, ‘Ravel’ and ‘Steiners Violetta’) and Asparagus wild relatives, for which the number of chromosomes, their size, the nuclear DNA content, and the genomic distribution of 5S and 45S rDNA were analyzed. Different ploidy levels (diploid, triploid, tetraploid, pentaploid and hexaploid) were found. Furthermore, the size of the chromosomes of all diploid Asparagus accessions was determined which led to differences in the karyotypic formula. A. plocamoides harbors the smallest chromosome with 1.21 μm, whereas the largest chromosome with 5.43 μm was found in A. officinalis. In all accessions one 5S rDNA locus per genome was observed, while the number of 45S rDNA loci varied between one (A. albus, A. plumosus, A. stipularis) to four (A. setaceus). In most Asparagus accessions, the 5S and 45S rDNA signals were located on different chromosomes. In contrast, the genomes of A. africanus, A. plocamoides, A. sp. (a taxonomically unclassified Asparagus species from Asia) and A. verticillatus (diploid accessions) have one 5S and one 45S rDNA signal on the same chromosome. The measured 2C DNA content ranges from 1.43 pg (A. plocamoides, diploid) to 8.24 pg (A. amarus, hexaploid). Intraspecific variations for chromosome number, karyotypic formula, signal pattern with 5S and 45s rDNA probes and DNA content were observed. Interspecific variations were also recognized in the genus Asparagus.

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