Genotypic and phenotypic determination of five virulence markers in clinical isolates of

Bollmann, R.; Seeburg, Anke; Parschau, Jana; Schönian, Gabriele; Sokolowska-Köhler, W; Halle, E. Von; Presber, W

doi:10.1016/s0928-8244(97)00015-1

FEMS Immunology and Medical Microbiology

1997

DOI: 10.1016/s0928-8244(97)00015-1

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Genotypic and phenotypic determination of five virulence markers in clinical isolates of

R. Bollmann¹,

Anke Seeburg²,

Jana Parschau³

et al.

Abstract: The presence of the virulence markers K1 capsule, serum resistance, aerobactin, S and P/PR fimbriae were examined in a total of 395 E. coli strains from different extraintestinal infections and in 81 faecal isolates of healthy volunteers using specific DNA probes and classical phenotypic methods. All markers were more frequently detected when genotypic assays were applied. The simultaneous occurrence of 3-4 virulence determinants was typical for isolates derived from patients with septicaemia or meningitis. Is… Show more

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Incidence of lysogenic, colicinogenic and siderophore-producing strains among human non-pathogenicEscherichia coli

2006

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The current incidence of Escherichia coli strains in healthy humans capable of producing the inhibitory exoproducts, such as temperate bacteriophages, corpuscular or HMW (high-molar mass) and proteinaceous or LMW (low-molar mass) colicins and siderophores was determined. Fifty-three E. coli strains were collected from the colons of 53 healthy human volunteers in Brno (Czechia) and tested for spontaneous and induced production of inhibitory exoproducts in a cross-test against each other. Of the strains tested, 37.7% produced bacteriophages, 41.5% produced from one to several LMW colicins, 11.3% formed HMW colicins and 15.1% (eight strains) produced exocellular siderophores different from enterochelin. Of these, seven strains formed aerobactin and one strain formed an untyped siderophore. E. coli strains differ greatly in the incidence of colicinogeny and lysogeny from its closest systemic relatives in the genus Escherichia and therefore should not be regarded as a model bacterium in this respect.

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Incidence of lysogenic, colicinogenic and siderophore-producing strains among human non-pathogenicEscherichia coli

2006

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Virulence factors in Escherichia coli from children with pyelonephritis

Draghijeva¹

2000

Clinical Microbiology and Infection

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The occurrence of bacterial infections of the urinary tract in children is exceeded in frequency only by bacterial infections of the respiratory tract. Acute pyelonephritis represents the most severe type of urinary tract infection in children. It not only results in greater morbidity but also has great potential for causing irreversible damage, including renal scarring, hypertension before the age of 30 years and pregnancy complications [1][2][3]. Escherichia coli causes most pyelonephritis (PN)-approximately 80-90% of first infections in children. The pathogenesis of E. coli pyelonephritis is complex, involving the interaction of several factors present in the host and in the invading organism. Host factors predisposing to PN can be anatomic, increased uroepithelial cell adherence, and nonsecretion of P blood group or of the Lewis blood group antigens [4]. Previous investigations have indicated that various virulence factors, such as pili associated with pyelonephritis (pap), afimbrial adhesin I, haemolysin (hly) and cytotoxic necrotizing factor 1 (cnf 1) are useful markers for the detection of uropathogenic E. coli and could therefore be used in the diagnosis of PN [5][6][7].The aim of this study was to determine the role of virulence factors in E. coli urinary isolates from children with pyelonephritis in University Children's Hospital, Sofia. One hundred and eighty E. coli strains were isolated from the urine of hospitalized children, ages between 5 months and 16 years of age, in the University Children's Hospital, Sofia. Midstream urine samples were collected after washing the external genitalia with sterile water.One hundred children had clinical acute pyelonephritis, with symptoms: fever, temperature ×38.5°C, flank pain, frequent chills, dysuria, frequency of urination, positive urine culture (×10 5 bacteria/mL urine), pyuria (×10 white blood cells/high power field), elevated erythrocyte sedimentation rate (×20 mm/h), an increased concentration of C-reactive protein (×20 mg/L) and reduced concentrating capacity. The clinical diagnosis of cystitis and urethritis was based on a history of frequency and urgency of urination, dysuria, suprapubic pain, and significant bacteriuria with the absence of fever, chills, and flank pain, and normal renal function.The control group comprised of 80 sex-and age-matched healthy children who had sterile urine cultures. Their E. coli strains were isolated from normal intestinal flora.Identification of E. coli was by standard bacteriological

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Genetic relationship between Escherichia coli strains isolated from the intestinal flora and those responsible for infectious diseases among patients hospitalized in intensive care units

Mereghetti¹,

Tayoro²,

Watt³

et al. 2002

Journal of Hospital Infection

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Genotypic and phenotypic determination of five virulence markers in clinical isolates of

Cited by 5 publications

References 0 publications

Incidence of lysogenic, colicinogenic and siderophore-producing strains among human non-pathogenicEscherichia coli

Incidence of lysogenic, colicinogenic and siderophore-producing strains among human non-pathogenicEscherichia coli

Virulence factors in Escherichia coli from children with pyelonephritis

Genetic relationship between Escherichia coli strains isolated from the intestinal flora and those responsible for infectious diseases among patients hospitalized in intensive care units

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