2006
DOI: 10.1007/bf02931581
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Incidence of lysogenic, colicinogenic and siderophore-producing strains among human non-pathogenicEscherichia coli

Abstract: The current incidence of Escherichia coli strains in healthy humans capable of producing the inhibitory exoproducts, such as temperate bacteriophages, corpuscular or HMW (high-molar mass) and proteinaceous or LMW (low-molar mass) colicins and siderophores was determined. Fifty-three E. coli strains were collected from the colons of 53 healthy human volunteers in Brno (Czechia) and tested for spontaneous and induced production of inhibitory exoproducts in a cross-test against each other. Of the strains tested, … Show more

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Cited by 11 publications
(9 citation statements)
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“…Detection of colicin activity was performed as described previously [38]. Briefly, the agar plates were inoculated with a stab from the bacterial culture ( Y. frederiksenii Y27601, E. coli TOP10F' carrying pDS1068, and E. coli TOP10F' carrying pBAD-A), incubated at 37°C for 48 hours and the resulting macrocolonies were killed with chloroform vapors.…”
Section: Methodsmentioning
confidence: 99%
“…Detection of colicin activity was performed as described previously [38]. Briefly, the agar plates were inoculated with a stab from the bacterial culture ( Y. frederiksenii Y27601, E. coli TOP10F' carrying pDS1068, and E. coli TOP10F' carrying pBAD-A), incubated at 37°C for 48 hours and the resulting macrocolonies were killed with chloroform vapors.…”
Section: Methodsmentioning
confidence: 99%
“…Detection of colicin production was performed as described previously (69). Briefly, the agar plates were inoculated by a stab of the producer being tested and incubated at 37°C for 48 h. The macrocolonies were killed using chloroform vapors (30-min exposure), and each plate was then overlaid with a thin layer of top agar containing 10 8 cells of an indicator strain.…”
Section: Methodsmentioning
confidence: 99%
“…A described set of E. coli indicator strains was used to identify the colicin and microcin types produced: E. coli K12-Row, C6 (ϕ), B1, P400, and Shigella sonnei 17 [1]; additionally, one recently verified indicator strain, E. coli S40, was also used [41]. Together, these indicator strains are capable of detecting all known colicin types including colicin L (P400) and colicin Js ( S.s .…”
Section: Methodsmentioning
confidence: 99%