Genotypic and phenotypic characterization of <i>Phytophthora infestans</i> populations on Java, Indonesia

Dangi, Sandesh; Wharton, P. S.; Ambarwati, Alberta Dinar; Santoso, Tri Joko; Kusmana,; Sulastrini, Ineu; Medendorp, John William; Hokanson, Karen; Douches, David S.

doi:10.1111/ppa.13269

Cited by 10 publications

(14 citation statements)

References 36 publications

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“…This is also shown by a low genetic differentiation among these populations. High genotypic diversity through sexual reproduction and oospores’ formation is common in neighboring countries such as Finland and the Baltic region [ 26 , 32 , 68 ], but differs from essentially clonal populations in western regions of Europe [ 72 , 73 , 74 ], and in the United States and South America [ 75 , 76 ], Asia [ 61 , 77 ], and Africa [ 78 , 79 ]. However, recent rapid local and global genotype shifts have occurred due to pathogen migration with tuber trade [ 80 , 81 ].…”

Section: Discussionmentioning

confidence: 99%

Highly Diverse Phytophthora infestans Populations Infecting Potato Crops in Pskov Region, North-West Russia

Runno-Paurson

Agho

Zoteyeva

et al. 2022

JoF

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There is limited understanding of the genetic variability in Phytophthora infestans in the major potato cultivation region of north-western Russia, where potato is grown primarily by small households with limited chemical treatment of late blight. In this study, the mating type, sensitivity to metalaxyl, and genotype and population genetic diversity (based on 12 simple sequence repeat (SSR) markers) of 238 isolates of P. infestans from the Pskov region during the years 2010−2013 were characterized. The aim was to examine the population structure, phenotypic and genotypic diversity, and the prevalent reproductive mode of P. infestans, as well as the influence of the location, time, and agricultural management practices on the pathogen population. The frequency of the A2 mating was stable over the four seasons and ranged from 33 to 48% of the sampled population. Both mating types occurred simultaneously in 90% of studied fields, suggesting the presence of sexual reproduction and oospore production in P. infestans in the Pskov region. Metalaxyl-sensitive isolates prevailed in all four years (72%), however, significantly fewer sensitive isolates were found in samples from large-scale conventional fields. A total of 50 alleles were detected in the 141 P. infestans isolates analyzed for genetic diversity. Amongst the 83 SSR multilocus genotypes (MLGs) detected, 65% were unique and the number of MLGs varied between locations from 3 to 20. These results, together with the high genotypic diversity observed in all the locations and the lack of significance of linkage disequilibrium, suggest that sexual recombination is likely responsible for the unique MLGs and the high genetic diversity found in the Pskov region population, resembling those of north-eastern European populations.

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Section: Discussionmentioning

confidence: 99%

Highly Diverse Phytophthora infestans Populations Infecting Potato Crops in Pskov Region, North-West Russia

Runno-Paurson

Agho

Zoteyeva

et al. 2022

JoF

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“…infestans population in Indonesia has been very little studied until recently; Nishimura et al (1999) reported on four isolates (all A2) collected in 1993 and two of these were further characterized by Gotoh et al (2005) as JP‐1. However, during 2016–2019, Dangi et al (2021) collected 140 FTA card samples of P . infestans from the three main potato‐growing areas of the island of Java, where 50% of Indonesia's potatoes are produced.…”

Section: Country Profilesmentioning

confidence: 99%

The dynamics of Phytophthora infestans populations in the major potato‐growing regions of Asia – A review

et al. 2021

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Asia is now the largest potato‐producing region of the world and late blight, caused by Phytophthora infestans, is the most important pathogen limiting production. This review documents, in both the historical and the current context, the population structure of P. infestans in the major areas of potato production in Asia. Information from diverse sources regarding the stated or inferred clonal pathogen lineages present, population changes, and possible migration routes of the pathogen into the countries of this region have been reviewed to aid researchers and those involved in managing late blight in Asia. The single most important factor for population change and resultant epidemics in this region has been found to be migration of pathogen genotypes from Europe and the Americas. Reducing the impact of such migration in the future will necessitate putting in place improved phytosanitary measures. To achieve this, data sharing using global networks such as AsiaBlight and EuroBlight is imperative.

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“…It was reported that the application of mancozeb was no longer effective in controlling leaf blight disease in tomato crops (Syahputra, 2018). Dangi et al (2020) also stated an indication of reduction sensitivity of…”

Section: Introductionmentioning

confidence: 99%

“…isolated from Colombia in 2018 reached its maximum growth on a 9 cm petri dish within 7 to 10 days. Meanwhile, P. infestans isolated from several regions in Indonesia including Garut in 2019 took about 7 to 10 days to fully cover the surface of the culture medium in a 9 cm petri dish(Dangi et al, 2020).…”

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confidence: 99%

In Vitro Sensitivity of Phytophthora infestans (Mont.) de Bary Isolated from Cikajang, Garut, to Several Fungicides

Widiantini

Maksum²,

Dono³

2022

Agrikultura

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Penyakit hawar daun yang disebabkan oleh oomycete Phytphthora infestans merupakan salah satu penyakit penting pada tanaman kentang. Metode pengendalian utama yang digunakan oleh petani adalah dengan menggunakan fungisida sintetis. Akan tetapi, penggunaan fungisida secara terus menerus dapat menyebabkan timbulnya populasi patogen yang resisten sehingga dapat menurunkan keefektifan fungisida tersebut. Penelitian ini dilaksanakan dengan tujuan untuk mengevaluasi sensitivitas P. infestans yang diisolasi dari pertanaman kentang di Desa Cikajang, Kabupaten Garut terhadap beberapa fungisida berbahan aktif metalaksil, mankozeb, dimetomorf, klorotalonil dan oksatiapiprolin. Eksperimen dilaksanakan dengan menggunakan metode makanan beracun dan detached-leaf assay. Hasil eksperimen menunjukkan bahwa P. infestans sensitif terhadap metalaksil dan mankozeb pada konsentrasi 2000 ppm dengan penghambatan pertumbuhan koloni masingmasing mencapai 93,3%. Lebih lanjut, berdasarkan hasil detached leaf assay, penghambatan terbentuknya lesi mencapai 100% pada perlakuan fungisida berbahan aktif metalaksil. Sementara penghambatan sebesar 70% diperoleh pada perlakuan fungisida berbahan aktif mankozeb. Sensitivitas P. infestans dikategorikan sebagai sedang terhadap fungisida berbahan aktif dimetomorf pada konsentrasi 1000 ppm dan klorotalonil pada konsentrasi 2000 ppm dengan penghambatan koloni masing-masing sebesar 65,5% dan 75,8% serta penghambatan pembentukan lesi 54,7% dan 59,1%. Eksperimen ini juga menemukan adanya indikasi penurunan sensitivitas P. infestans terhadap oksatiapiprolin yang ditunjukkan dengan rendahnya penghambatan pertumbuhan yang hanya sebesar 8,5% dengan penghambatan pembentukan lesi sebesar 48,8% ketika dibandingkan dengan keempat jenis fungisida lainnya.

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Genotypic and phenotypic characterization of Phytophthora infestans populations on Java, Indonesia

Cited by 10 publications

References 36 publications

Highly Diverse Phytophthora infestans Populations Infecting Potato Crops in Pskov Region, North-West Russia

Highly Diverse Phytophthora infestans Populations Infecting Potato Crops in Pskov Region, North-West Russia

The dynamics of Phytophthora infestans populations in the major potato‐growing regions of Asia – A review

In Vitro Sensitivity of Phytophthora infestans (Mont.) de Bary Isolated from Cikajang, Garut, to Several Fungicides

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