Genotypes of the <i>Helicobacter pylori vacA</i> Signal Sequence Differ with Age in Korea

Lee, Jun Haeng; Choe, Yon Ho; Jeon, Byung Ha; Oh, Yoo Joung; Kim, Jae J.; Rhee, Jong Chul; Kim, Pum Soo; Choi, Mi Sook

doi:10.1111/j.1083-4389.2004.00198.x

Cited by 6 publications

(3 citation statements)

References 29 publications

(43 reference statements)

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“…In Portugal and Tunisia, virulent strains have been detected in adult patients more frequently than children [ 36 ]. It has been described that H. pylori strains experiment recombination with others more virulent and better adapted strains to host changes, resulting in genotypes variable distribution between age groups [ 37 , 38 ].…”

Section: Discussionmentioning

confidence: 99%

Prevalence ofHelicobacter pylori vacA,cagA, andiceAGenotypes in Cuban Patients with Upper Gastrointestinal Diseases

Feliciano

Gutiérrez

Valdés

et al. 2015

BioMed Research International

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Virulence factors of Helicobacter pylori can predict the development of different gastroduodenal diseases. There are scarce reports in Cuba about H. pylori isolates genotyping. The aim of the present investigation was to identify allelic variation of the virulence genes vacA, cagA, and iceA in sixty-eight patients diagnosed as H. pylori positive by culture. In seven out of 68 patients, strains from both gastric regions were obtained and considered independent. DNA was extracted from all the H. pylori strains and evaluated by PCR-genotyping. The vacA s1 allele, cagA gene, and iceA2 allele were the most prevalent (72.0%, 56.0%, and 57.3%, respectively). Alleles from m-region showed a similar frequency as s1a and s1b subtypes. The presence of multiple H. pylori genotypes in a single biopsy and two gastric region specimens were found. Significant statistical association was observed between iceA2 allele and patients with non-peptic ulcer dyspepsia (NUD) (P = 0.037) as well as virulence genotypes (s1, s1m2) and patients over 40 years old (P < 0.05). In conclusion, the results demonstrated a high prevalence of H. pylori virulent genotypes in Cuban patients over 40 years old while iceA2 alleles demonstrated a good specificity in patients with NUD.

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Section: Discussionmentioning

confidence: 99%

Prevalence ofHelicobacter pylori vacA,cagA, andiceAGenotypes in Cuban Patients with Upper Gastrointestinal Diseases

Feliciano

Gutiérrez

Valdés

et al. 2015

BioMed Research International

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“…Primer sequences for PCR amplifications of the 16S rRNA gene and for the genotypes cagA, vacA and iceA were designed based on published papers (Ho et al, 1991;Van Doorn et al, 1998b;Lee et al, 2004) with a modification of PCR mixtures and annealing temperatures (Table 1). Each PCR was carried out in a total volume of 25 μL containing 3-4 μL (approximately 350 ng) of genomic DNA from gastric biopsies, 3 μL (30 pmol) each of forward and reverse primers, 12.5 μL of 2X master mix (Promega) and required volume of nuclease free water.…”

Section: Pcr Assays For H Pylori Identification and Determination Ofmentioning

confidence: 99%

PCR-based analysis of Helicobacter pylori virulent genotypes among dyspeptic patients from Chittagong, Bangladesh

Habib¹,

Alam²,

Rudra³

et al. 2017

MJM

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Aims:Helicobacter pylori is a causative agent of gastroduodenal diseases in Bangladesh as well as throughout the world. This study aimed to determine the H. pylori cagA, vacA and iceA virulent genotypes by PCR directly in gastric biopsies from dyspeptic patients of Chittagong, Bangladesh and evaluating the association of these genotypes with clinical manifestations. Methodology and results: CLO (Campylobacter-Like Organism) test and Hp16s PCR (16S rRNA based H. pylori specific PCR) was performed to confirm H. pylori infection. Among 111 patients, H. pylori infection was found in 60 patients by CLO test, while Hp16s PCR revealed that 54 patients were H. pylori positive. PCR amplification of the H. pylori virulence genes was successful in 35 gastric biopsies amongst the 54 Hp16s PCR positive biopsies. The positive rates for the cagA, vacAs1, vacAs2, vacAm1, vacAm2, iceA1, iceA2 genes were 34.3%, 71.4%, 8.6%, 62.9%, 28.6%, 20% and 11.4%, respectively. The allelic variant vacAs1m1 had a predominant percentage with 51.4%, followed by vacAs1m2, vacAs2m2 and vacAs1m1m2 with 14.3%, 5.7% and 2.9%, respectively. Among the subtypes of vacAs1, only s1a was detected in 54.3% of biopsies while none of the cases showed the s1b and s1c genotypes. However, there was no statistically significant association (P>0.05) observed between the virulent genotypes and clinical conditions. Conclusion, significance and impact of study: We found that cagA, vacAs1m1 and iceA1 were the most frequent H. pylori genotypes in severe clinical outcomes of the infection. The data in this study would provide a basis for understanding the diverse virulence pattern of this bacterium in Bangladeshi dyspeptic patients.

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“…The primers used in this study were Korean specific VASK-F (5'-MTKRTTTCTCT-CGCTTT-3') and VASK-R (5'-GGGATYTGTATAAGTCGT-ATT-3'). 15 All samples stored were reexamined for vacA s1.…”

Section: Dna Identification Of H Pylorimentioning

confidence: 99%

Ghrelin Levels in Gastric Mucosa before and after Eradication of Helicobacter pylori

Choe¹,

Lee²,

Lee³

et al. 2007

Gut Liver

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Genotypes of the Helicobacter pylori vacA Signal Sequence Differ with Age in Korea

Abstract: Strain populations infecting Korean adults and children differ.

Cited by 6 publications

References 29 publications

Prevalence ofHelicobacter pylori vacA,cagA, andiceAGenotypes in Cuban Patients with Upper Gastrointestinal Diseases

Prevalence ofHelicobacter pylori vacA,cagA, andiceAGenotypes in Cuban Patients with Upper Gastrointestinal Diseases

PCR-based analysis of Helicobacter pylori virulent genotypes among dyspeptic patients from Chittagong, Bangladesh

Ghrelin Levels in Gastric Mucosa before and after Eradication of Helicobacter pylori

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