Genotype diversity of group A rotavirus strains in children with acute diarrhea in urban Burkina Faso, 2008–2010

Abstract

In this study, the diversity of G and P genotypes of rotavirus strains in Burkinabe children were examined. Between November 2008 and February 2010, 447 stool samples were collected from children <5 years of age with acute diarrhea visiting hospital in Ouagadougou. Group A rotavirus was previously detected in 151/447 (33.8%) of the samples tested by an immunochromatographic test and these samples were now tested further for rotavirus G and P genotypes by RT‐PCR. Of these, the rotavirus type genes were amplified by RT‐PCR for 140/151 (92.7%) samples and G and P genotypes were successfully determined for 81 (57.9%) and 130 (92.9%) samples, respectively. The most prevalent G genotypes were G1, 34/140 (24.3%), and G9, 21/140 (15%), while the predominant P genotypes were P[6], 56/140 (40%), and P[8], 54/140 (38.6%). Among the single infections, 63/140 (45%), the predominant G/P combinations were: G1P[8] (33%), G9P[8] (29%), and G2P[6] (14%). The unusual strains G1P[9] (3%), G12P[6] (3%), G10P[6] (2%), and G2P[8] (2%) were also detected. In a high number of strains 61/140 (43.6%), the G genotype could not be determined and mixed infections were determined in 17/140 (12.1%) of strains identified. This study highlights the high diversity and presence of unusual rotavirus strains in children in Burkina Faso. J. Med. Virol. 83:1485–1490, 2011. © 2011 Wiley‐Liss, Inc.

Rotavirus in diarrheal children in rural Burkina Faso: High prevalence of genotype G6P[6]

“…The G2 and P[4] types are not present in the monovalent G1P[8] vaccine, and it has been suggested that the vaccine shows a lesser efficiency to this type (Gurgel et al, 2007). During this RVA season however, the G2P[4] RVs were not highly prevalent and a study from Burkina Faso the previous year showed a similar prevalence of G2 strains (Bonkoungou et al, 2011).…”

“…In Burkina Faso, previous studies have observed a high burden of RVA disease in children with high RVA prevalence during the cold dry season December-February (Bonkoungou et al, 2010(Bonkoungou et al, , 2011Nitiema et al, 2011;Steele et al, 2010). Furthermore, in a previous study from the capital Ouagadougou, RVA strains were characterized by their G and P-types (Nordgren et al, 2012).…”

Acute Diarrhea in West African Children: Diverse Enteric Viruses and a Novel Parvovirus Genus

“…A prior study analyzing feces from children Ͻ5 years of age with acute diarrhea reported that 34% of them were positive for rotavirus by an immunochromatographic assay (SD Bioline Rota/Adeno; Standard Diagnostics, Inc., Kyonggi-Do, South Korea) (12,13). Samples were collected between November 2008 and February 2010 from the capital city of Ouagadougou in Burkina Faso (12).…”

“…Here we report on a viral metagenomic analysis of feces of children from Burkina Faso with acute diarrhea (12) allowing the characterization of their enteric viruses, including the genetic characterization of novel parvoviruses from a previously unrecognized genus (12,13).…”

New insights into rotavirus vaccines

“…Moreover, in developing countries, some authors highlight the existence of significant diversity of rotavirus strains with unusual type combinations that could influence RV vaccine effectiveness. 62,63 Recently Gladstone et al showed that early infection and frequent reinfection in a setting with high viral diversity resulted in lower protection than has been reported elsewhere, providing a possible explanation for the observed lower than expected efficacy of rotavirus vaccine in Asia and Africa. 64 …”