Rotavirus in diarrheal children in rural Burkina Faso: High prevalence of genotype G6P[6]

Nordgren, Johan; Bonkoungou, Isidore Juste O.; Nitiema, Léon W.; Sharma, Sumit; Ouermi, Djénèba; Simporé, Jacques; Barro, Nicolas; Svensson, Lennart

doi:10.1016/j.meegid.2012.08.014

Cited by 30 publications

(28 citation statements)

References 36 publications

(66 reference statements)

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“…This 5-year study clearly showed that there is a high rotavirus genotype variation, with 33 G-P combinations identified over the time in Yaoundé, Cameroon. Genotype G1[P8] was the most detected genotype within the overall study period and is known to be the most common strain globally; however, unusual combinations like G9P[6], G2P[6], G3P[6], G8P[6], G6P[6] were detected and this reaffirmed the circulation of such strains in Africa, as previously reported in West Africa (Akran et al, 2010;Armah et al, 2010b;Nordgren et al, 2012). The genotype G12P[8] was also detected and has been circulating in Yaoundé since 2007.…”

Section: Discussionsupporting

confidence: 78%

Molecular surveillance of rotavirus strains circulating in Yaoundé, Cameroon, September 2007–December 2012

Boula

Waku‐Kouomou

Kinkela

et al. 2014

Infection, Genetics and Evolution

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Section: Discussionsupporting

confidence: 78%

Molecular surveillance of rotavirus strains circulating in Yaoundé, Cameroon, September 2007–December 2012

Boula

Waku‐Kouomou

Kinkela

et al. 2014

Infection, Genetics and Evolution

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“…Although P[6] was initially considered of zoonotic origin [18] and assigned to SGI [18, 57], the combination G1P[6] was shown to belong to SGII, as also shown before in the previous Angolan study [22], and in the same subgroup as the well-studied G1P[8]. …”

Section: Discussionmentioning

confidence: 78%

Characterization of rotavirus infection in children with acute gastroenteritis in Bengo province, Northwestern Angola, prior to vaccine introduction

Gasparinho¹,

Piedade

Mirante³

et al. 2017

PLoS ONE

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BackgroundRotavirus group A (RVA) is considered the leading cause of pediatric diarrhea, responsible for the high burden of diarrheal diseases in sub-Saharan Africa. Despite recent studies, the existent data are scarce for some African countries like Angola, a country with one of the highest RVA-related death estimates. The aim of this study was to determine the RVA detection rate and circulating genotypes in children less than five years of age with acute gastroenteritis attended at the Bengo General Hospital in Caxito, Bengo province, Angola, before vaccine introduction.MethodsBetween September 2012 and December 2013, 342 fecal specimens were collected from children enrolled. Positive samples for RVA by immunochromatographic rapid test were G and P-typed by hemi-nested type-specific multiplex PCR, and subgrouped for the VP6 gene. VP4 and VP7 genes from a subset of samples were sequenced for phylogenetic analysis.ResultsDuring the study period, a high RVA detection rate was registered (25.1%, 86/342).The age group most affected by RVA infection includes children under 6 months of age (p<0.01). Vomiting was highly associated with RVA infection (72.1%; p<0.001).From the 86 RVA-positive samples, 72 (83.7%) were genotyped. The most prevalent genotype was G1P[8] (34/72; 47.2%), followed by the uncommon G1P[6] (21/72; 29.2%), and G2P[4] (9/72; 12.5%). Only two G-types were found: G1 (60/72; 83.3%) and G2 (11/72; 15.3%). Among the P-genotypes, P[8] was the most prevalent (34/72; 47.2%), followed by P[6] (22/72; 30.6%) and P[4] (9/72; 12.5%). In the phylogenetic trees, the identified G and P-types clustered tightly together and with reference sequences in specific monophyletic groups, with highly significant bootstrap values (≥92%).ConclusionThis pre-vaccination study revealed, for the first time for Bengo province (Angola), the RVA genotype profile, including phylogenetic relationships, and a high RVA detection rate, supporting the immediate introduction of a RVA vaccine in the national immunization programme.

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“…Specific virus–host interactions have also been demonstrated by resolving the atomic structures of VP8* proteins for select human RVs (P[14] and P[11]) in complex with their HBGA oligosaccharide ligands with X-ray crystallography 28, 38 and STD NMR analyses (P[19]). 39 The associations between RV infection and a child's secretor status and Lewis types have also been observed through epidemiology and literature data studies, 24, 26, 27, 40, 41, 42 strongly suggesting that HBGAs play an important role in RV infection, host range and pathogenesis.…”

Section: Introductionmentioning

confidence: 74%

Histo-blood group antigens as receptors for rotavirus, new understanding on rotavirus epidemiology and vaccine strategy

Jiang

Liu

Tan

2017

Emerging Microbes & Infections

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The success of the two rotavirus (RV) vaccines (Rotarix and RotaTeq) in many countries endorses a live attenuated vaccine approach against RVs. However, the lower efficacies of both vaccines in many low- and middle-income countries indicate a need to improve the current RV vaccines. The recent discovery that RVs recognize histo-blood group antigens (HBGAs) as potential receptors has significantly advanced our understanding of RV diversity, evolution and epidemiology, providing important new insights into the performances of current RV vaccines in different populations and emphasizing a P-type-based vaccine approach. New understanding of RV diversity and evolution also raises a fundamental question about the ‘Jennerian' approach, which needs to be addressed for future development of live attenuated RV vaccines. Alternative approaches to develop safer and more cost-effective subunit vaccines against RVs are also discussed.

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Rotavirus in diarrheal children in rural Burkina Faso: High prevalence of genotype G6P[6]

Cited by 30 publications

References 36 publications

Molecular surveillance of rotavirus strains circulating in Yaoundé, Cameroon, September 2007–December 2012

Molecular surveillance of rotavirus strains circulating in Yaoundé, Cameroon, September 2007–December 2012

Characterization of rotavirus infection in children with acute gastroenteritis in Bengo province, Northwestern Angola, prior to vaccine introduction

Histo-blood group antigens as receptors for rotavirus, new understanding on rotavirus epidemiology and vaccine strategy

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