Genotype Characterization of Newcastle Disease Virus Isolated from Commercial Chicken Flocks in West Java, Indonesia

Putri, Dwi Desmiyeni

doi:10.29261/pakvetj/2018.041

Cited by 11 publications

(17 citation statements)

References 24 publications

(35 reference statements)

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“…Poor biosecurity and inadequate control strategies are the leading causes of NDV transmission in commercial poultry and wild birds [ 15 ]. Putri et al [ 11 ] noted that in recent years, there are two genotypes of NDV that are always present in Indonesia; these are genotype II and genotype VII. The NDV belonging to the sub-genotype VII.2 is known as the fundamental cause of infectious ND at poultry in Indonesia [ 10 , 11 ], and genotype II NDV, in particular, LaSota, is used as a seed in a live vaccine [ 12 ].…”

Section: Discussionmentioning

confidence: 99%

“…NDV isolates from Indonesia have been typed as genotypes 1, II, VI, and VII based on the fusion ( F ) gene phylogeny [ 9 - 12 ]. Isolates belonging to sub-genotype VIIh and VIIi, now namely sub-genotype VII.2, have also been recently reported [ 3 , 11 – 13 ]. The NDV, which is an RNA virus, can mutate rapidly, giving rise to new sub-genotypes.…”

Section: Introductionmentioning

confidence: 99%

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Phylogenetic studies of Newcastle disease virus isolated from poultry flocks in South Sulawesi Province, Indonesia, in 2019

Saputri¹,

Poetri²,

Soejoedono³

2021

J Adv Vet Anim Res

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Objective: Indonesia is one of the Newcastle disease (ND) endemic countries in the world. An outbreak of the ND virus (NDV) was first reported in Indonesia in 1926. This study aimed to detect, isolate, and classify the NDV by molecular approaches from poultry farms in South Sulawesi Province of Indonesia in 2019. Materials and Methods: As many as 36 pooling samples from the cloacal swab, trachea swab, proventriculus, and spleen tissues obtained from ND-suspected chickens were isolated in 11-day-old embryonated chicken eggs type-specific antibody-negative. The viruses were confirmed by reverse transcription-polymerase chain reaction (RT-PCR), followed by sequencing. Results: The results showed that 18 out of 36 pooling samples were NDV-positive based on the isolation result and RT-PCR test. The sequencing results showed that 10 NDV isolates had a motif 112 R-R-Q-K-R-F 117 in the fusion protein cleavage site region, which suggested that the NDV isolates were of virulent pathotype. The phylogenetic studies based on the F gene’s partial nucleotide sequence classified the study isolates into NDV virus genotype/subgenotype VII.2. Conclusion: These findings are expected to help provide the latest characteristic information of NDV in South Sulawesi Province to determine the seed vaccine for control strategies of ND.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Phylogenetic studies of Newcastle disease virus isolated from poultry flocks in South Sulawesi Province, Indonesia, in 2019

Saputri¹,

Poetri²,

Soejoedono³

2021

J Adv Vet Anim Res

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“…Two NDVs were used in this study. First virus was NDV/Ck/BGR/2011 obtained from repository of the Laboratory of Immunology, Faculty of Veterinary Medicine, Bogor Agricultural University, which categorized as virulent NDV and belongs to genotype VII NDV [ 11 , 12 ]. Another virus was Sato NDV which obtained from the National Veterinary Drug and Assay Laboratory, Gunung Sindur, Bogor, Indonesia.…”

Section: Methodsmentioning

confidence: 99%

Production and characterization of Newcastle disease antibody as a reagent to develop a rapid immunodiagnostic test tool

Putri¹,

Handharyani

Soejoedono

et al. 2018

Vet World

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Aim:This research was conducted to produce and characterize ND antibody as reagent candidate to develop a rapid immunodiagnostic test tool.Materials and Methods:Four New Zealand White rabbits were used in this study and divided into two groups. First group was injected by Sato ND antigen, and second group was injected by genotype VII ND antigen. This study is divided into three steps: (a) ND antibody production, (b) ND antibody purification, and (c) ND antibody characterization. First group was rabbit injected by Sato NDV (5×108.25 egg lethal doses (ELD)50/ml) and second group was injected by genotype VII NDV (5×106.5 ELD50/ml). Antigen induction was performed by subcutaneous administrated for first (day 1) and second (day 14) injection and intravenous administrated for third (day 30) injection. Blood was collected on day 8 after third injection.Results:Antibody production increased on second antigen injection and reached a peak on day 9 after second antigen injection. Sato and genotype VII ND antibody can be produced without adjuvant within 38 days with the highest titer 210. Based on antibody titer data, both antigens induced antibody production in a similar trend. The characterization antibody by SDS-PAGE indicated that molecular weight of immunoglobulin G (IgG) is 154.93 kDa (whole IgG), heavy chain 54.39 kDa, and light chain 27.74 kDa. ND antibodies have specificity to homologous and heterologous NDVs in varying virulence.Conclusion:Sato and genotype VII ND antibodies have been successfully produced within 38 days without adjuvant. Specificity of ND antibodies to NDVs in varying virulence and cross-reaction between Sato ND antibody and genotype VII ND antibody indicates that the characterized ND antibodies can be used as a reagent to develop rapid immunodiagnostic test tools.

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“…Interaction between viruses and the environment, including the host immune system, resulted in NDV evolution and continues to produce new genotypes of viruses. Lately, infection of genotype VII NDV has caused high mortality of birds in several poultry farms in Indonesia [ 13 , 14 ]. Recently, producing hyperimmune serum in animals has become an essential activity of many research projects.…”

Section: Introductionmentioning

confidence: 99%

Production of hyperimmune serum against genotype VII Newcastle disease virus in rabbits with several applications

Putri¹,

Poetri²,

Candra³

et al. 2022

J Adv Vet Anim Res

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Objective: This study aimed to produce hyperimmune serum against genotype VII Newcastle disease virus (NDV) with several applications. Materials and Methods: Production of hyperimmune serum against genotype VII NDV was performed on eight New Zealand white rabbits divided into four groups. Rabbits were immunized three times on the 1st day, the 14th day, and the 30th day. Blood sampling was carried out on the 8th day after the third immunization. Results: All groups showed the same pattern of hemagglutination inhibition (HI) titer results. HI titers would peak on the 5th or the 9th day after the second immunization, then decrease until the 3rd day after the third immunization, and increase again on the 5th day after the third immu¬nization. Rabbits immunized intravenously showed higher HI titers than the other groups. These results indicate that the intravenous route for hyperimmune serum production against genotype VII Newcastle disease virus greatly affects the immune response result. Conclusions: The production of hyperimmune serum by intravenous immunization three times was able to produce the highest titer of 210 at 38 days. The agar gel precipitation test and the Western blot assay showed that the hyperimmune serum was specific for the Newcastle disease antigen.

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Genotype Characterization of Newcastle Disease Virus Isolated from Commercial Chicken Flocks in West Java, Indonesia

Cited by 11 publications

References 24 publications

Phylogenetic studies of Newcastle disease virus isolated from poultry flocks in South Sulawesi Province, Indonesia, in 2019

Phylogenetic studies of Newcastle disease virus isolated from poultry flocks in South Sulawesi Province, Indonesia, in 2019

Production and characterization of Newcastle disease antibody as a reagent to develop a rapid immunodiagnostic test tool

Production of hyperimmune serum against genotype VII Newcastle disease virus in rabbits with several applications

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