Genotype and medium affect shoot regeneration of melon

Abstract

The effects of basal media, growth regulators and gelling agents on the morphogenetic response of eleven Cucumis melo L. var. reticulatus and inodorus genotypes were examined. Regeneration was achieved from cultured cotyledons of all genotypes and the morphogenic response was affected by the genetic background. Among the combination of factors tested, MS basal medium supplemented with 2.8 gM 6-benzyladenine (BA) and 1.0 BM abscisic acid (ABA) solidified with agar gave the highest frequency of shoot regeneration.

Agrobacterium-mediated transformation and shoot regeneration in elite breeding lines of western shipper cantaloupe and honeydew melons (Cucumis melo L.)

“…At this writing, there were no transformation studies available for honeydew melon; however, a number of studies on shoot regeneration in culture have been reported (Ficcadenti and Rotino 1995;Keng and Hoong 2005;Kintzios and Taravira 1997;Oridate et al 1992;Orts et al 1987). In western shipper cantaloupe, one microprojectile mediated transformation (Gonsalves et al 1994) and two Agrobacterium-mediated transformations (Clough and Hamm 1995;Fuchs et al 1997) were reported to confer resistance to cucumber mosaic virus (CMV), zucchini yellow mosaic virus (ZYMV), and watermelon mosaic virus (WMV).…”

“…The synthetic auxin 2,4-dichlorophenoxyacetic acid has been widely used to induce somatic embryogenesis (AkasakaKennedy et al 2004;Gray et al 1993;Guis et al 1997;Kintzios et al 2004;Kintzios and Taravira 1997;Oridate and Oosawa 1986). In addition, 6-(c,c-dimethylallylamino)-purine (2iP), gibberellic acid (GA 3 ), kinetin, thidiazuron and a-naphthaleneacetic acid have been used in melon culture (Fang and Grumet 1990;Ficcadenti and Rotino 1995;Guis et al 2000;Souza et al 2006;Yadav et al 1996). The effects of these growth regulators on melon regeneration differed depending on plant genotype, explant type and culture conditions.…”

Cefotaxime and Benzyladenine Improve Melon Regeneration

“…The different concentration of BA that were reported in studies on melon could possibly be due to the variations in the endogenous hormone levels among the different cultivars. Additionally, different plant genotype and varieties exhibit quite different regeneration rates (18). Explant age and gelling agents may also affect the melon regeneration efficiency (19).…”

“…Based on our preliminary investigation even ½ MS medium without auxin has the potential of the root induction (data not shown). This could be due to the fact that different melon cultivars might respond to the different concentration of the plant growth regulators (18).…”

In vitro Regeneration of Sweetgourd (Cucurbita moschata Duch.)

“…The importance of BAP for organogenesis had been studied in cucurbits (Curuk et al 2002, Lee et al 2003) and it was mentioned as an exclusive requirement for shoot formation. BAP was also reported superior to TDZ (Ficcadenti and Rotino 1995) for melon (Cucumis melo). Therefore, four different concentrations of BAP were used in this study to further optimize the regeneration protocol of sweetgourd.…”